National University of Pharmacy
Kharkiv, Ukraine
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Techen N.,National University of Pharmacy | Parveen I.,National University of Pharmacy | Khan I.A.,National University of Pharmacy | Khan I.A.,King Saud University
Current Opinion in Biotechnology | Year: 2014

Because of the increasing demand for herbal remedies and for authentication of the source material, it is vital to provide a single database containing information about authentic plant materials and their potential adulterants. The database should provide DNA barcodes for data retrieval and similarity search. In order to obtain such barcodes, several molecular methods have been applied to develop markers that aid with the authentication and identification of medicinal plant materials. In this review, we discuss the genomic regions and molecular methods selected to provide barcodes, available databases and the potential future of barcoding using next generation sequencing. © 2013 Elsevier Ltd.

Chaban V.V.,University of Rochester | Savchenko T.I.,National University of Pharmacy | Kovalenko S.M.,National University of Pharmacy | Prezhdo O.V.,University of Rochester
Journal of Physical Chemistry B | Year: 2010

Hydrophobicity and the ability to absorb light that penetrates through living tissues make carbon nanotubes (CNTs) promising intracellular drug delivery agents. Following insertion of a drug molecule into a CNT, the latter is delivered into a tissue, is heated by near-infrared radiation, and releases the drug. To assess the feasibility of this scheme, we investigate the rates of energy transfer between CNT, water, and the drug molecule and study the temperature and concentration dependence of the diffusion coefficient of the drug molecule inside CNTs. We use ciprofloxacin (CIP) as a sample drug: direct penetration of CIP through cell membranes is problematic due to its high polarity. The simulations show that a heated CNT rapidly deposits its energy to CIP and water. All estimated time scales for the vibrational energy exchange between CNT, CIP, and water are less than 10 ps at 298 K. As the system temperature grows from 278 to 363 K, the diffusion coefficient of the confined CIP increases 5-7 times, depending on CIP concentration. The diffusion coefficient slightly drops with increasing CIP concentration. This effect is more pronounced at higher temperatures. The simulations support the idea that optical heating of CNTs can assist in releasing encapsulated drugs. © 2010 American Chemical Society.

BACKGROUND: Several antibiotics have shown promising anti-malarial effects and have been useful for malarial chemotherapy, particularly in combination with standard anti-malarial drugs. Tigecycline, a semi-synthetic derivative of minocycline with a unique and novel mechanism of action, is the first clinically available drug in a new class of glycylcycline antibiotics.METHODS: Tigecycline was tested in vitro against chloroquine (CQ)-sensitive (D6) and resistant strains (W2) of Plasmodium falciparum alone and in combination with CQ. Tigecycline was also tested in vivo in combination with CQ in Plasmodium berghei-mouse malaria model for parasitaemia suppression, survival and cure of the malaria infection.RESULTS: Tigecycline was significantly more active against CQ-resistant (W2) than CQ-susceptible (D6) strain of P. falciparum. Tigecycline potentiated the anti-malarial action of CQ against the CQ-resistant strain of P. falciparum by more than seven-fold. Further, treatment of mice infected with P. berghei with tigecycline (ip) produced significant suppression in parasitaemia development and also prolonged the mean survival time. Treatment with as low as 3.7 mg/kg dose of tigecycline, once daily for four days, produced 77-91% suppression in parasitaemia. In vivo treatment with tigecycline in combination with subcurative doses of CQ produced complete cure in P. berghei-infected mice.CONCLUSION: Results indicate prominent anti-malarial action of tigecycline in vitro and in vivo in combination with CQ and support further evaluation of tigecycline as a potential combination candidate for treatment of drug-resistant cases of malaria.

Prada G.I.,National University of Pharmacy
Acta Endocrinologica | Year: 2014

Researches on ageing phenomenon offer significant information regarding the consequences of stressors on immune system that affects longevity in the elderly. Immunosenescence has become the most common immunodeficiency state in humans, occurring in over 30% of community - dwelling elderly, and greater than 90% of elderly who are ill, taking medication, or residing in longterm care facilities. Immunosenescence may reflect tandem changes in neuroendocrine responses. There are several agingrelated changes in cortisol, DHEA and catecholamines, which are considered to set up a “vicious cycle of endocrinosenescence and immuno-senescence”. The low-level, chronic increase in innate, inflammatory response observed in older adults ultimately results in tissue damage and disease; the key inflammatory mediators in this process are CRP, nuclear factor (NF)-kB, IL-1-beta, IL-6, TNF-alpha, cyclooxygenase-2 (COX-2), and inducible nitric oxide (NO) synthase. Further, glucocorticoid inhibition of IL-6 production was observed to be lower in older compared to younger men following psychological stress. There are individual differences that protect aged people from stressors and strains, and it will be important to identify biological mechanisms of protection and those at risk who might benefit from early behavioral interventions. © 2014, Acta Endocrinologica Foundation. All rights reserved.

Ntirenganya C.,University of Kigali | Manzi O.,University of Kigali | Muvunyi C.M.,National University of Pharmacy | Ogbuagu O.,Yale University
American Journal of Tropical Medicine and Hygiene | Year: 2015

Antimicrobial resistance (AMR) is a serious public health threat in both developed and developing countries. Many developing countries, including Rwanda, lack adequate surveillance systems, and therefore, the prevalence of AMR is not well-known. We conducted a prospective observational study to assess the prevalence of AMR among common bacterial isolates from clinical specimens obtained from patients on the medical wards of Kigali University Teaching Hospital (KUTH). We evaluated the antibiotic sensitivity patterns of bacterial pathogens cultured from urine, blood, sputum, and wound swab specimens obtained over a 6-month period (July 1 toDecember 30, 2013). There were 154 positive cultures from specimens obtained from 141 unique patients over the study period. Urine, blood, wound swab, and sputum cultures comprised 55.2%, 25.3%, 16.2%, and 3.3% of the total specimens evaluated; 31.4% and 58.7% of Escherichia coli and Klebsiella isolates, respectively, were resistant to at least one of the third generation cephalosporins. Eight percent of E. coli isolates were resistant to imipenem; 82% and 6% of Staphylococcus aureus strains were oxacillin-and vancomycin-resistant respectively. Antimicrobial resistance rates are high in Rwanda and pose a serious therapeutic challenge to the management of common infections. Copyright © 2015 by The American Society of Tropical Medicine and Hygiene.

Sheykina N.V.,National University of Pharmacy
9th International Kharkiv Symposium on Physics and Engineering of Microwaves, Millimeter and Submillimeter Waves, MSMW 2016 | Year: 2016

It was revealed experimentally that at small level of magnetic field's noise (less than 4μT/Hz0.5) the dependence of gravitropic reaction of cress roots on frequency had a fine structure. The peak that corresponded to the cyclotron frequency of Ca2+ ions for the static component of combined magnetic field that was equal to 40μT became split up into three peaks (f1 = 31/3Hz, f2 = 32.5Hz H f3 = 34 Hz). The frequency f1 corresponds to Ca2+ ion (the theoretical value is 31.6 Hz), the frequency f2 corresponds to hydronium ion H3O+ (the theoretical value is 32.9 Hz), and frequency f3 relates OH- ion (the theoretical value is 35 Hz), Taking into account the influence of combined magnetic field on the hydronium ions and the Del Giudice hypothesis the main doubts about the existence of ion cyclotron resonance may be broken. Hydronium ions are unusual because they have a long free path. It was revealed that the combined magnetic field tuned for the cyclotron frequency of hydronium ion changed pH of pure water. Such changes have to lead to biological effects. © 2016 IEEE.

Park H.Y.,Korea University | Park H.Y.,National University of Pharmacy | Kim G.-Y.,Jeju National University | Choi Y.H.,Korea University | Choi Y.H.,University Graduate Center
International Journal of Molecular Medicine | Year: 2012

Naringenin, one of the most abundant flavonoids in citrus fruits and grapefruits, has been reported to exhibit anti-inflammatory and antitumor activities. However, the cellular and molecular mechanisms underlying the naringenin anti-inflammatory activity are poorly understood. In this study, we conducted an investigation of the inhibitory effects of naringenin on the production of lipopolysaccharide (LPS)-induced pro-inflammatory mediators in BV2 microglial cells. We found that pre-treatment with naringenin prior to treatment with LPS significantly inhibited excessive production of nitric oxide (NO) and prostaglandin E 2 (PGE 2) in a dose-dependent manner. The inhibition was associated with downregulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Naringenin also attenuated the production of pro-inflammatory cytokines and chemokines, including interleukin-1β (IL-1β), tumor necrosis factor-α(TNF- α) and monocyte chemoattractant protein-1 (MCP-1) by suppressing expression of mRNAs for these proteins. In addition, the molecular mechanism underlying naringenin-mediated attenuation in BV2 cells has a close relationship to suppressing translocation of the nuclear factor-κB (NF-κB) p65 subunit into the nucleus and the phosphorylation of Akt and mitogen-activated protein kinases (MAPKs). These findings suggest that naringenin may provide neuroprotection through suppression of pro-inflammatory pathways in activated BV2 microglial cells.

Pogorelov S.V.,National University of Pharmacy
Springer Series in Optical Sciences | Year: 2015

Measurement methods of parameters and characteristics of intense (up to several tens of kilowatts in continuous mode and up to several tens of kilojoules in pulse mode) and wide-aperture laser radiation with bolometric gauges have been considered. The bolometric gauges are able towork in wide spectral range, withstand high levels of laser power or pulse energy. The considered methods use bolometric grids, which are loop-through type gauges and consume a low part of laser radiation. The maximal size of input aperture of bolometric grids has no limitations. Nonlinearities of bolometer transformation characteristics have been considered. © Springer Science+Business Media Dordrecht 2015.

Zdoryk O.A.,National University of Pharmacy
International journal of pharmaceutical compounding | Year: 2013

Pharmaceutical compounding in modern Ukraine has a rich history and goes back to ancient times. Today in the Ukraine, there is a revival of compounding practice, the opening of private compounding pharmacies, updating of legislative framework and requirements of the State Pharmacopeia of Ukraine for compounding preparations, and the introduction of Good Pharmaceutical Practice.

Tovchiga O.V.,National University of Pharmacy
BMC Complementary and Alternative Medicine | Year: 2016

Background: Diabetes mellitus and metabolic syndrome are the common problems of the modern society. The interest in herbal medicines increases, and often they are used in combination with conventional drugs. Aegopodium podagraria L. (goutweed) is a plant widely used in traditional medicine. Hypoglycemic effect of goutweed aerial part tincture has been previously shown in alloxan-induced diabetic mice and in rats receiving excess of fructose and hydrochlorothiazide. The effects of co-administration of the tincture with widely used antihyperglycemic drugs have not been verified. The objective of this study is to determine the efficacy of goutweed tincture and its combination with metformin using the model reproducing the pathogenetic mechanisms of the metabolic syndrome and type 2 diabetes. Methods: The animals were divided into 5 groups, as follows: intact control, dexamethasone (untreated), dexamethasone + metformin, 50 mg/kg; dexamethasone + A. podagraria tincture, 1 ml/kg intragastrically; dexamethasone + metformin, 50 mg/kg intragastrically + A. podagraria tincture, 1 ml/kg intragastrically. Dexamethasone was used at a dose of 5 mg/kg subcutaneously for 5 days. Insulin tolerance test and oral glucose tolerance test were performed, triglycerides, total lipids, total and HDL cholesterol content in plasma were determined, LDL cholesterol content was calculated, glycogen content in the liver was measured. Results: Goutweed tincture combined with metformin increased its effect on the basal glycemia and on the results of the short insulin test. In the oral glucose tolerance test the lowest area under glucose curve and average glycemia value were seen in animals receiving this combination. Only metformin tended toward the reduction of liver glycogen. The decrease in triglycerides and increment of HDL cholesterol content (caused by the tincture), as well as tendency towards the decrease in total lipids level (caused by metformin) were observed against a background of the investigated combination, though the ability of GW tincture to reduce LDL cholesterol content and the same tendency seen against a background of metformin were eliminated when these preparations were administered together. Conclusion: It has been shown that goutweed tincture combined with the respectively low dose of metformin partially increases the efficacy of the latter in dexamethasone-treated rats. Graphical abstract: Goutweed tincture combined with the respectively low dose of metformin partially increases the efficacy of the latter in dexamethasone-treated rats. © 2016 The Author(s).

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