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Bratislava, Slovakia

Pecivova J.,Slovak Academy of Sciences | Macickova T.,Slovak Academy of Sciences | Svitekova K.,National Transfusion Service | Nosal R.,Slovak Academy of Sciences
Interdisciplinary Toxicology | Year: 2012

Activated neutrophils represent the main source of myeloperoxidase (MPO), superoxide (SO) and subsequently derived oxygen metabolites. They have important microbicidal activities, however in inflammatory conditions they may secondarily attack surrounding tissues. Overproduction of reactive oxygen species, prolonged or excessive liberation of MPO and other effective yet also toxic substances from neutrophils may participate in disturbed apoptosis, intensify the inflammatory processes and result in serious human diseases. The inhibitory effect of quercetin on PMA stimulated SO generation in isolated human neutrophils was found to be dosedependent, without affecting the activity of intact isolated neutrophils. At comparable conditions, quercetin was more potent in inhibiting MPO release than SO generation. Our results indicate that quercetin could support resolution of inflammation through decreased activity of neutrophils, i.e. respiratory burst and degranulation. Copyright © 2012 SETOX & IEPT, SASc.

Macickova T.,Slovak Academy of Sciences | Pecivova J.,Slovak Academy of Sciences | Harmatha J.,Czech Institute of Organic Chemistry And Biochemistry | Svitekova K.,National Transfusion Service | Nosal R.,Slovak Academy of Sciences
Interdisciplinary Toxicology | Year: 2012

Neutrophils represent the body's primary line of defense against invading pathogens. They most rapidly reach the site of injury or infection, liberate antimicrobial proteins, proteases and produce reactive oxygen species. Prolonged or excessive liberation of these very effective and toxic substances could intensify the inflammatory process and enhance tissue damage in many diseases, such as allergies, infections and rheumatoid arthritis. Pterostilbene belongs to stilbenoids, structural analogues of resveratrol, which act as natural protective agents in defending the plant against viral and microbial attack. It possesses anticancerous, antidiabetic and anti-inflammatory properties. The study provides new information on the effect of pterostilbene [0.01-100 μmol/l] on superoxide generation in and myeloperoxidase (MPO) release from azurophil granules of isolated human neutrophils. PMA [1 μmol/l], which activates NADPH-oxidase via protein kinase C, was used for stimulation of neutrophils Unstimulated cells showed neither superoxide generation nor myelopereoxidase release after preincubation with the drug studied. Pterostilbene dose dependently decreased superoxide generation in and MPO release from stimulated human neutrophils, however a significant decrease was recorded only in the concentration 100 μmol/l. The effect of pterostilbene was more pronounced on superoxide generation in comparison to MPO release. Our results suggest that the effect of pterostilbene may prove beneficial in controlling inflammation. Copyright © 2012 SETOX & IEPT, SASc.

Nosal R.,Slovak Academy of Sciences | Perecko T.,Slovak Academy of Sciences | Jancinova V.,Slovak Academy of Sciences | Drabikova K.,Slovak Academy of Sciences | And 2 more authors.
Neuroendocrinology Letters | Year: 2010

OBJECTIVE: Neutrophil leukocytes and macrophages represent professional phagocytic cells. When appropriately stimulated, they undergo dramatic physiological and biochemical changes resulting in phagocytosis, chemotaxis and degranulation with the activation of reactive oxygen species (ROS) production known as the respiratory burst. DESIGN: In this study we analysed the effect of a crystalline complex fraction of four N-feruloyl-serotonin isomers isolated from the seeds of Leuzea carthamoides on the mechanism of oxidative burst of human neutrophils in vitro. RESULTS: N-feruloyl-serotonin (N-f-5HT) inhibited dose-dependently oxidative burst of human whole blood and isolated neutrophils in vitro stimulated with phorbol-myristate-acetate (PMA) as measured by luminol/isoluminol enhanced chemiluminescence. In isolated neutrophils stimulated with PMA, N-f-5HT was effective against extracellular as well as intracellular reactive oxygen species. Western blot analysis documented that N-f-5HT in concentrations of 10 and 100 μMe; significantly decreased PMA-induced phosphorylation of protein kinase C alpha/beta II. CONCLUSION: The results suggest that N-f-5HT represents an effective naturally occurring substance with potent effect on the oxidative burst of human neutrophils and could be further investigated for its pharmacological activity against oxidative stress in ischaemia-reperfusion, inflammation and other pathological conditions. © 2010 Neuroendocrinology Letters.

Ferianec V.,Comenius University | Krizko Jr. M.,Comenius University | Papcun P.,Comenius University | Svitekova K.,National Transfusion Service | And 3 more authors.
Neuroendocrinology Letters | Year: 2011

OBJECTIVE: To outline possibility of successful treatment of spontaneous previable rupture of membranes in the second trimester of pregnancy. Spontaneous previable rupture of membranes (SPROM) in the second trimester of pregnancy is one of the most alarming problems in current obstetrics. Perinatal mortality is about 60%, one third of which represents intrauterine fetal demise. Surviving neonates suffer from various complications. There are different clinical approaches regarding treatment of SPROM. MATERIAL & METHODS: We present a case of a 30 year old secundigravida with a history of SPROM at 19+1 weeks gestation. Ultrasonographic examination revealed anhydramnios. Genital cultures and laboratory studies ruled out infectious etiology of SPROM. Due to expected poor neonatal outcome, decision to attempt amniopatch as an experimental therapeutic alternative was made at 21+1 weeks gestation (two weeks after SPROM had occurred). Autologous concentrated platelets followed by autologous cryoprecipitate were administered into the amniotic cavity transabdominally under ultrasound guidance. After 3 days sonographic examination showed normal volume of amniotic fluid. On 22 postoperative day, patient notice some leaking of fluid vaginally. Fetal growth was appropriate, amniotic fluid volume was decreased, however, oligohydramnios never progressed to anhydramnios. Pregnancy ended with primary cesarean delivery at 33+1 weeks gestation. Live born male infant with 1750 g birth weight was delivered. Postnatal development was within normal limits. CONCLUSION: Intraamniotic application of "amniopatch" may represent a possibly successful treatment of spontaneous previable rupture of membranes. This case reports the longest stop of the leaking of amniotic fluid and total prolongation of pregnancy with favorable perinatal outcome after "amniopatch" treatment of spontaneous previable rupture of membranes in the second trimester so far published in available literature. © 2011 Neuroendocrinology Letters.

Jancinova V.,Slovak Academy of Sciences | Perecko T.,Slovak Academy of Sciences | Nosal R.,Slovak Academy of Sciences | Svitekova K.,National Transfusion Service | Drabikova K.,Slovak Academy of Sciences
Oxidative Medicine and Cellular Longevity | Year: 2013

Neutrophils are able to release cytotoxic substances and inflammatory mediators, which, along with their delayed apoptosis, have a potential to maintain permanent inflammation. Therefore, treatment of diseases associated with chronic inflammation should be focused on neutrophils; formation of reactive oxygen species and apoptosis of these cells represent two promising targets for pharmacological intervention. Piceatannol, a naturally occurring stilbenoid, has the ability to reduce the toxic action of neutrophils. This substance decreased the amount of oxidants produced by neutrophils both extra- and intracellularly. Radicals formed within neutrophils (fulfilling a regulatory role) were reduced to a lesser extent than extracellular oxidants, potentially dangerous for host tissues. Moreover, piceatannol did not affect the phosphorylation of p40phox - a component of NADPH oxidase, responsible for the assembly of functional oxidase in intracellular (granular) membranes. The stilbenoid tested elevated the percentage of early apoptotic neutrophils, inhibited the activity of protein kinase C (PKC) - the main regulatory enzyme in neutrophils, and reduced phosphorylation of PKC isoforms α, βII, and δ on their catalytic region. The results indicated that piceatannol may be useful as a complementary medicine in states associated with persisting neutrophil activation and with oxidative damage of tissues. © 2013 Viera Jancinova et al.

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