Time filter

Source Type

Sun C.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Xu C.,Henan Agricultural University | Zhao M.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Huang Y.,Henan Provincial Tobacco Corporation | And 2 more authors.
Acta Tabacaria Sinica | Year: 2015

C4-AgNPs were synthesized by using new chitosan Schiff base derivatives with the structure of quatemary ammonium salt as reducing agent and stabilizer to develop new anti-TMV agent which was efficient, low toxic and environmental friendly. Inhibitory effect of s C4-AgNPs on TMV and its biochemical mechanism were also studied. Results showed that 17 μg/mL C4-AgNPs had good prevention and treatment effects on TMV, and it could effectively reduce the number of lesion with prevention rate up to 86.95%. C4-AgNPs could improve activity of defense enzymes catalase (CAT) and phenylalanine ammonialyase (PAL). Meanwhile, the content of proline was enhanced and that of MDA reduced by tracing the changes of physiological and biochemical indexes in a certain period of time on Nicotiana tabacum var. K326. © 2015, Acta Tabacaria Sinica Editorial Department. All right reserved.


Sun W.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Wang J.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Zhou J.,Beijing Cigarette Factory | Ma Y.,Beijing Cigarette Factory | And 5 more authors.
Acta Tabacaria Sinica | Year: 2015

Field experiment was conducted to determine the effect of nitrate nitrogen content on TSNAs formation during high temperature storage. Different concentration of nitrate was applied in tobacco growing season. Leaf samples from lamina and midrib of both burley and flue-cured tobacco were collected. Lab experiment was conducted in a temperature-controlled chamber at 45℃ and 10℃ for 15d. Nitrate nitrogen and TSNA contents of all samples were determined. Results indicated that nitrate nitrogen content increased with the increment of nitrogen fertilizer. Content of nitrate nitrogen and TNSA had a low correlation in newly cured tobacco. However, after high temperature storage for 15d, the correlation significantly increased. Nitrate nitrogen content and TSNAs increment during high temperature storage showed a significant positive correlation. Nitrate nitrogen content in midrib was much higher than that in lamina in both burley and fluecured tobacco. After high temperature storage for 15d, TSNAs increment in midrib was significantly higher than that in lamina. With the increase of nitrate applied to flue-cured tobacco leaves, nitrate nitrogen and TSNA content after high temperature storage increased significantly. These results indicated that nitrate nitrogen content was closely related to the formation of TSNAs during high temperature storage. Therefore, reduction of leaf nitrate content through agronomic measures is effective in reducing TSNA formation during storage. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


Sun W.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Yang J.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | Zhou J.,Beijing Cigarette Factory | Xu D.,National Tobacco Cultivation & Physiology & Biochemistry Research Center | And 4 more authors.
Acta Tabacaria Sinica | Year: 2015

A pot experiment was conducted to study the effect of different ratio of nitrate-N to ammonium-N on nitrate nitrogen contents, chemical composition and TSNA formation during high temperature storage. Results showed that nitrate nitrogen contents of burley tobacco increased with the increment of the ratio of nitrate-N to ammonium-N. Contents of 4 types of TSNA and total contents of TSNAs after curing also significantly increased with the increase of the ratio of nitrate-N to ammonium-N. In the process of air-curing, nitrate nitrogen contents significantly increased. After 45℃ storage for 15d, the contents of 4 types of TSNAs and total contents of TSNAs were all significantly higher than that stored at low temperature. The net increase of TSNA contents also increased with the ratio of nitrate-N to ammonium-N. In the process of maturing and curing of flue-cured tobacco, nitrate nitrogen contents decreased. Flue-cured tobacco leaves contained low contents of nitrate nitrogen after curing. Nitrate nitrogen contents and TSNA contents before and after high temperature storage in flue-cured tobacco had no relationship with the ratio of nitrate-N to ammonium-N, while the TSNA level was the lowest under the treatment with the nitrate-N to ammonium-N ratio of 50 to 50 for middle leaves and under the treatment of 100% nitrate-N for upper leaves. With the increase of ammonium-N to nitrate-N ratio, the contents of nicotine, total nitrogen and protein of burley tobacco increased significantly. Chemical composition of flue-cured tobacco showed no relationship with nitrogen source, but the flue-cured leaves under treatment of NO3 --N to NH4 +-N ratio of 50: 50 had the highest sugar content and well-balanced chemical composition. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

Loading National Tobacco Cultivation & Physiology & Biochemistry Research Center collaborators
Loading National Tobacco Cultivation & Physiology & Biochemistry Research Center collaborators