Entity

Time filter

Source Type


Angelova S.,National Specialized Hospital for Active Therapy of Hematological Diseases | Spassov B.,National Specialized Hospital for Active Therapy of Hematological Diseases | Nikolova V.,National Specialized Hospital for Active Therapy of Hematological Diseases | Christov I.,Medical University-Pleven | And 2 more authors.
Cytology and Genetics | Year: 2015

The aim of our study was (1) to define if the amplification of c-MYC, MLL and RUNX1 genes is related to the progressive changes of the karyotype in patients with AML and MDS with trisomy 8, 11, and 21 (+8, +11, and +21) in bone marrow and (2) can that amplification be accepted as part of the clonal evolution (CE). Karyotype analysis was performed in 179 patients aged 16–81 with AML or MDS with different chromosomal aberrations (CA). The findings were distributed as follows: initiating balanced CA (n = 60), aneuploidia (n = 55), unbalanced CA (n = 64). Amplification of c-MYC, MLL and RUNX1 genes by means of fluorescence in situ hybridization (FISH) was found in 35% (7 out of 20) of AML and MDS patients with +8, +11, and +21 as a single CA in their karyotype; in 63.6% of the pts (7 out of 11)—with additional numerical or structural CA and in 75% (9 out of 12)—with complex karyotype. We assume that the amplification of the respective chromosomal regions in patients with +8, +11, and +21 is related to CE. Considering the amplification as a factor of CE, we established 3 patterns of karyotype development depending on the type of the initiating CA in it. Significant statistical differences were found between the three patterns regarding the karyotype distribution in the different stages of progression (p < 0.001). © 2015, Allerton Press, Inc.


Angelova S.,National Specialized Hospital for Active Therapy of Hematological Diseases | Spassov B.,National Specialized Hospital for Active Therapy of Hematological Diseases | Nikolova V.,National Specialized Hospital for Active Therapy of Hematological Diseases | Christov I.,Medical University-Pleven | And 2 more authors.
Cytology and Genetics | Year: 2015

The aim of our study was to assess if the type of primary chromosomal aberrations (CA) of the karyotype of patients with Acute myeloid leukemia (AML) and Myelodysplastic syndromes (MDS) determines the way and the rate of karyotype development. Conventional cytogenetic analysis was carried out on 248 AML and 105 MDS patients at diagnosis. Clonal evolution (CE) was found in 40% (51 of 128) of AML patients and in 47.5% (19 of 40) of MDS patients having CA in their karyotype. The first pattern we established was for the most frequent CA which initiate CE in 28 patients with a complex karyotype. These CA were non-balanced rearrangements in the following regions: 5q, 7q, 11q, 3q, monosomy 5, monosomy 7. The second pattern of CE was regarding the most frequent aneuploidias (+8, +11, +21, −Y), and the third pattern concerned balanced CA. We found significant difference in the distribution of karyotypes in different stages of progression between the first and the other two groups (p < 0.001). No statistical difference was found between the patterns in the second and the third group CA (p > 0.5). © 2015, Allerton Press, Inc.

Discover hidden collaborations