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Reeves A.B.,U.S. Geological Survey | Poulson R.L.,University of Georgia | Muzyka D.,National Scientific Center Institute of Experimental and Clinical Veterinary Medicine | Ogawa H.,Obihiro University of Agriculture and Veterinary Medicine | And 6 more authors.
Infection, Genetics and Evolution | Year: 2016

Avian paramyxovirus serotype 4 (APMV-4) is a single stranded RNA virus that has most often been isolated from waterfowl. Limited information has been reported regarding the prevalence, pathogenicity, and genetic diversity of AMPV-4. To assess the intercontinental dispersal of this viral agent, we sequenced the fusion gene of 58 APMV-4 isolates collected in the United States, Japan and the Ukraine and compared them to all available sequences on GenBank. With only a single exception the phylogenetic clades of APMV-4 sequences were monophyletic with respect to their continents of origin (North America, Asia and Europe). Thus, we detected limited evidence for recent intercontinental dispersal of APMV-4 in this study. © 2015.

Szadziewski R.,University of Gdask | Dominiak P.,University of Gdask | Filatov S.,National Scientific Center Institute of Experimental and Clinical Veterinary Medicine
Zootaxa | Year: 2015

Alluaudomyia canariensis Szadziewski & Dominiak sp. nov. from the Canary Islands and A. wyskokensis Szadziewski & Dominiak sp. nov. from Poland and Ukraine are described and illustrated. The genus Alluaudomyia is reported from the Canary Islands for the first time. The article is supplemented with a checklist and an identification key for the species so far recorded from Europe and the Canary Islands.

Limanskaya O.,Ukrainian Academy of Sciences | Limanskaya O.,National Scientific Center Institute of Experimental and Clinical Veterinary Medicine | Limanskii A.,Ukrainian Academy of Sciences | Limanskii A.,Kyoto University
Biophysics (Russian Federation) | Year: 2012

Complexes of bacteriophage T7 RNA polymerase with a DNA template for transcription elongation were visualized by atomic force microscopy. Images for complexes of T7 RNA polymerase with terminal fragments of DNA template were obtained for single molecules. Complexes of a single DNA template molecule with several T7 RNA polymerase molecules corresponding to stages of initiation, elongation and termination of transcription were visualized under the elimination of unspecific DNA-protein binding. Immobilized on the amino mica RNA transcripts form rod-like condensed structures. Detailes of specific and unspecific complex formation for the T7 RNA polymerase-DNA system during initiation and transcription elongation are discussed. © 2012 Pleiades Publishing, Ltd.

Limanskaya O.Yu.,Ukrainian Academy of Sciences | Limanskaya O.Yu.,National Scientific Center Institute of Experimental and Clinical Veterinary Medicine | Fesenko T.N.,Ukrainian Academy of Sciences | Pokrovskiy V.A.,Ukrainian Academy of Sciences | And 4 more authors.
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2011

Point mutations associated with isoniazid resistance inMycobacterium tuberculosis (MTB) have been analyzed in codon 315 of the katG gene by conventional polymerase chain reaction (PCR) using primers containing locked nucleic acid (LNA) modified nucleotides. Purity and structure of primers containing 5 LNA monomers of 17 nucleotides in length were characterized by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and a 17-mer duplex formed by two complementary oligonucleotides was characterized by the method of thermal denaturation. The duplex containing five LNA monomers per each strand was characterized by a higher melting temperature than it was expected using extrapolation of theoretical calculation for nucleotide modification of one strand of the duplex. Detection of any of six possible mutations in katG codon 315 (i.e. discrimination between sensitive and resistant MTB) requires just one PCR employing a set of two primers with one LNA-modified primer; this is an important advantage of oligonucleotides containing LNA over unmodified nucleotides: employment of multiplex PCR would require up to 12 primers. Problems of control of oligonucleotide modification by LNA monomers are discussed. © Pleiades Publishing, Ltd., 2011.

Rola-Luszczak M.,National Veterinary Research Institute | Pluta A.,National Veterinary Research Institute | Olech M.,National Veterinary Research Institute | Donnik I.,Urals State Scientific Research Institute of Veterinary Medicine | And 5 more authors.
PLoS ONE | Year: 2013

Recent studies have shown that bovine leukemia virus (BLV) sequences can be classified into seven distinct genotypes based on full gp51 sequence. This classification was based on available sequence data that mainly represented the BLV population that is circulating in cattle from the US and South America. In order to aid with a global perspective inclusion of data from Eastern Europe is required. In this study we examined 44 BLV isolates from different geographical regions of Poland, Belarus, Ukraine, and Russia. Phylogenetic analysis based on a 444bp fragment of env gene revealed that most of isolates belonged to genotypes 4 and 7. Furthermore, we confirmed the existence of a new genotype, genotype 8, which was highly supported by phylogenetic computations. A significant number of amino acid substitutions were found in the sequences of the studied Eastern European isolates, of which 71% have not been described previously. The substitutions encompassed mainly the C-part of the CD4+ epitope, zinc binding peptide region, CD8+ T cell epitope, and overlapping linear epitope E. These observations highlight the use of sequence data to both elucidate phylogenetic relationships and the potential effect on serological detection of geographically diverse isolates. © 2013 Rola-Łuszczak et al.

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