Scherbinina S.P.,National Scientific Center for Hematology |
Levina A.A.,National Scientific Center for Hematology |
Lisovskaya I.L.,National Scientific Center for Hematology |
Ataullakhanov F.I.,National Scientific Center for Hematology
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2012
In many diseases associated with impairments in iron metabolism, erythrocytes exhibit an increased sensitivity to oxidative stress induced in vitro. In this study we have examined the antioxidant status of erythrocytes from healthy donors and from 12 patients with disorders of iron homeostasis by measuring the extent of hemolysis induced in vitro by tert-butyl hydroperoxide (t-BHP). The extent of hemolysis observed with patient erythrocytes was significantly higher than that observed in experiments with erythrocytes from healthy donors. After therapeutic infusions of the antioxidants mexidol or emoxypin, oxidative hemolysis in patients was restored to normal values and blood hepcidin increased significantly as compared with its initial level. A significant correlation was observed between hepcidin concentration after treatment and t-BHP-induced hemolysis before treatment. These data suggest that antioxidants may exert a favorable effect on those at risk for iron overload disease. © 2012 Pleiades Publishing, Ltd.