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Fedenko E.S.,National Research Institute of Immunology | Elisyutina O.G.,National Research Institute of Immunology | Filimonova T.M.,National Research Institute of Immunology | Boldyreva M.N.,National Research Institute of Immunology | And 4 more authors.
Self/Nonself - Immune Recognition and Signaling | Year: 2011

objectives: Atopic dermatitis (AD) is an increasingly common, chronic, relapsing, infammatory skin disease characterized by impaired epidermal barrier function and cutaneous infammation. the prevalence of AD has steadily increased during the past few decades. the aim of this study was to comparatively investigate cytokine gene expression in the skin and peripheral blood of atopic dermatitis patients and healthy individuals.Results: In the skin of patients with AD, a signifcant increase of the level of gene expression was observed for inter-leukin (IL)-2r (p < 0.0023), IL-5 (p = 0.002), IL-6 (p < 0.0023), IL-8 (p = 0.01), IL-12B (p < 0.0023), IL-10 (p < 0.0023), IL-23 (p = 0.002), IL-29 (p < 0.0023), and transforming growth factor beta (TGFbeta) (p < 0.0023) as compared to healthy individuals. In contrast, no diference between AD patients and healthy donors was detected with respect to cytokine gene expression in the peripheral blood.Methods: Samples of skin and peripheral blood from 48 severe AD patients (SCoRAD = 78.5 [57;89], IGA = 4.2 [3,9;4,7]) at the age of 17 to 45 years and 20 healthy donors aged from 19 to 32 years were analyzed for gene expression of cyto-kines using real-time reverse transcription polymerase chain reaction (Rt-pCR).Conclusions: Activity of markers of chronic infammation and th1 immune response in severe AD, namely IL-2r, IL-8, IL-12B, IL-23, IL-29 and TGFbeta, as well as activity of anti-infammatory IL-5 were predominant in the skin but not in the blood of AD patients. © 2011 Landes Bioscience.

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