National Research Institute of Ayurvedic Drug Development

Bhubaneshwar, India

National Research Institute of Ayurvedic Drug Development

Bhubaneshwar, India
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Das D.,National Research Institute of Ayurvedic Drug Development | Mitra A.,National Research Institute of Ayurvedic Drug Development | Datta D.,P.A. College | Saha A.,University of Calcutta | Hazra J.,National Research Institute of Ayurvedic Drug Development
International Journal of Research in Ayurveda and Pharmacy | Year: 2012

In the modern medical system, pyrexia is not only considered as a disease, but Jwara or pyrexia has been depicted as a cardinal disease from the period of Brihatrayee (2000B.C - 7th Century A.D.) due to its severeity and worsening prognosis. The vivic classification of the disease, Jwara has been acertained in different traditional Ayurvedic texts and the treatment are being elaborately described in these texts, but most are the compond formulation pertaining to endangered species. Considering this, Parusaka (Grewia asiatica Linn.) has been selected as a drug having the Jwaraghna (antipyretic) action and Vedananasaka (analgesic). The basis for the pharmacological action is based on panachabhautika composition. This study was carried out to evaluate its analgesic and antipyretic activities in experimental animal model. The aqueous extract of Parusaka was administered in different doses to swiss albino mice / rats and compared for analgesic - antipyretic activity by administering aspirin. The extract (doses of 200-300mg / kg body weight) showed good analgesic effect due to its inhibitory effects on pain induced by acetic acid in writhing test (Chemical stimulation) and tail immersion test (Thermal stimulation). The trial drug (300mg /kg body weight) also produced significant increase in the hot plate reaction time (when compared to aspirin 400mg/kg body weight in mice) indicating its analgesic effects. The extract containing 400mg /kg body weight dose had significant effect than aspirin (100mg / kg body weight) on reducing pyrexia, induced by administering lipopolysaccharide extract from E. Coli. This study therefore support the use of Parusaka as indigenous analgesic and antipyretic agent in India and further investigations is required to elucidate its pharmacodynamic action.

Nanda G.C.,National Research Institute of Ayurvedic Drug Development
International Journal of Research in Ayurveda and Pharmacy | Year: 2013

Guggulu a very known and common name in the field of Ayurvedic practices which is used as an important medicine for treating a lot of diseases. The drug having a lot of potency described in Ayurveda with pharmacological action tested with its present Research and Development study. It should be wiser to establish a platform for the next generation to understand the modules of Guggulu preparations and administrations. Though guggulu is an established drug but the phytochemical and Pharmacological R and D study have found the merits and demerits of the guggulusterone, the active principle of the drug Guggulu. However, the relationship between the effect of Guggulu both in Ayurveda and modern science has been found similar. Efforts have been made in this paper to study the relationship of Guggulu starting from Veda to present Lab. facts.

Dhar B.L.,National Research Institute of Ayurvedic Drug Development | Kumar R.K.,National Research Institute of Ayurvedic Drug Development | Manosi D.,National Research Institute of Ayurvedic Drug Development | Jayram H.,National Research Institute of Ayurvedic Drug Development
International Journal of Pharmacognosy and Phytochemical Research | Year: 2016

A sensitive and reliable high performance thin layer chromatographic method has been developed for quantification of quercetin in a polyherbal compound for urolithiasis. Methanolic extract of Crataeva nurvala and Bryophyllum pinnatum was chromatographed on silica gel 60F254 Aluminum plates plates with Chloroform: Methanol: Formic acid in the ratio (7.5:1.5:1; v/v/v) as mobile phase. The Quercitin quantification was 1.82% (w/w) and 3.2 % (w/w) for Crataeva nurvala Buch. -Ham and Bryophyllum pinnatum Lam. respectively. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.

Nag G.,University of Calcutta | Das S.,University of Calcutta | Mandal S.,National Research Institute of Ayurvedic Drug Development | De B.,University of Calcutta
Pharmacognosy Journal | Year: 2015

Aim: The aim of the study was to analyze the antioxidant, anti-amylase, anti-glucosidase and antiacetylcholinesterase (anti-AChE) properties of the leafy shoots of three Indian species of Swertia e.g. Swertia chirata and its substitutes Swertia bimaculata, and Swertia decussata, their xanthones and amarogentin. Methods: Antioxidant activity of the methanolic extracts of leafy shoots was measured in terms of DPPH, superoxide and nitric oxide radical scavenging activities as well as metal chelating properties. Enzyme inhibitory properties were measured using AChE, α-amylase and α-glucosidase respectively. Five xanthones bellidifolin (1), swerchirin (2), decussatin (3), mangiferin (4) and 1-hydroxy-3,5,8-trimethoxy xanthone (6) and one iridoid, amarogentin (5) were isolated from Swertia chirata. The activities of the isolated components were compared. Results: Swertia chirata exhibited best antioxidant and anti-AChE properties than the other two species. The plants also possessed α-glucosidase inhibitory properties but weak α-amylase inhibitory activity. Highest activities were observed in Swertia chirata. We report here, for the first time, the antioxidant, anti-AChE and anti-glycosidase activity of 1-hydroxy-3,5,8-trimethoxy xanthone. This xanthone had strongest DPPH radical scavenging activity and anti-AChE property. Conclusion: The results suggest the beneficial effects of the xanthones of Swertia chirata. But further study should be carried out to prove the efficacy in vivo.

Kumar R.K.,National Research Institute of Ayurvedic Drug Development | Dhar B.L.,National Research Institute of Ayurvedic Drug Development | Jayram H.,National Research Institute of Ayurvedic Drug Development
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Ayurveda intervention is gaining popularity for chronic and degenerative disorders. Lumbar stenosis is narrowing of spaces of lumbar spine cause morbidity in old age. Surgical laminectomy is the only answer in conventional therapy. However, the disease can be better managed in Ayurveda through panchakarma and rasayana treatment. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.

Kumar P.,Hamdard University | Bhandari U.,Hamdard University | Jamadagni S.,National Research Institute of Ayurvedic Drug Development
BioMed Research International | Year: 2014

This study investigated the inhibitory effect of aqueous extract of Trigonella foenum-graecum seeds (AqE-TFG) on fat accumulation and dyslipidemia in high fat diet- (HFD-) induced obese rats. Female Wistar rats were fed with HFD ad libitum, and the rats on HFD were treated orally with AqE-TFG or orlistat ((HFD for 28 days + AqE-TFG (0.5 and 1.0 g/kg) or orlistat (10 mg/kg) from day 8 to 28), respectively. Treatment with AqE-TFG produced significant reduction in body weight gain, body mass index (BMI), white adipose tissue (WAT) weights, blood glucose, serum insulin, lipids, leptin, lipase, and apolipoprotein-B levels and elevation in adiponectin levels. AqE-TFG improved serum aspartate amino transferase (AST), alanine amino transferase (ALT), and lactate dehydrogenase (LDH) levels. AqE-TFG treatment reduced the hepatic and cardiac thiobarbituric acid reactive substances (TBARS) and elevated the antioxidant enzyme (glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)) levels. In addition, liver and uterine WAT lipogenic enzyme (fatty acid synthetase (FAS) and glucose-6-phosphate dehydrogenase (G6PD)) activities were restored towards normal levels. These findings demonstrated the preventive effect of AqE-TFG on fat accumulation and dyslipidemia, due to inhibition of impaired lipid digestion and absorption, in addition to improvement in glucose and lipid metabolism, enhancement of insulin sensitivity, increased antioxidant defense, and downregulation of lipogenic enzymes. © 2014 Parveen Kumar et al.

Harwansh R.K.,Jadavpur University | Mukherjee K.,Jadavpur University | Bhadra S.,Jadavpur University | Kar A.,Jadavpur University | And 3 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Trikatu is a very well known 'Rasayana' in Ayurveda and widely used as a polyherbal ayurvedic formulation in India. It consists of three well known plants, viz.; Piper longum (PL), Piper nigrum (PN) and Zingiber officinale (ZO) in equal ratio. Trikatu has been prescribed for cough, cold, fever, asthma, respiratory problems and improvement of digestive disorders. The aim of the present study was to investigate the effect of individual ingredients of trikatu namely PL, PN, and ZO and formulations [Marketed formulation (MF) and laboratory formulation (LF)] on drug metabolizing enzymes (CYP3A4 and CYP2D6), to assess its herb-drug interaction potential through cytochrome P450 inhibition assays. Further this work was aimed to develop an RP-HPLC method for the identification and quantification of piperine and 6-gingerol in the crude drug trikatu. Materials and methods Enzyme inhibition effect of LF, MF, PL, PN and ZO was explored through CYP450-CO complex assay using rat liver microsomes (RLM) and a fluorescence screening method using individual isoenzymes (CYP3A4 and CYP2D6). The RP-HPLC method was developed for the identification and quantification of piperine and 6-gingerol in LF, MF and individual plant materials at the concentration of 1 mg/mL. Results RP-HPLC analysis confirmed the presence of piperine and 6-gingerol in LF and MF [Piperine: 7.89±2.12% (w/w) (MF), 6.70±2.13% (w/w) (LF)]; [6-gingerol: 5.3±1.21% (w/w) (MF), 4.95±2.34% (w/w) (LF)]. Inhibitory potential of MF and LF in CYP450-CO complex assay was found to be 37.54±3.12% (MF) and 35.12±2.31% (LF) and against CYP2D6 and CYP3A4 was estimated to be IC50 251.30±3.98 and 245.23±1.92 μg/mL and IC50 225.50±1.02 and 223.254±0.92 μg/mL respectively. Conclusions Different concentrations of the trikatu formulation and its individual components showed significantly (p<0.001) less inhibitory activity on individual isoenzymes as compared to the positive control. The crude drug exhibited inhibitory potential against the CYP450 enzymes in a concentration dependent manner. Outcome of the present study demonstrated that trikatu has less interaction potential with drug metabolizing enzymes. © 2014 Elsevier Ireland Ltd.

Jamadagni S.B.,National Research Institute of Ayurvedic Drug Development | Jamadagni P.S.,National Research Institute of Ayurvedic Drug Development | Lacy S.H.,Joint Pathology Center | Williams B.,Joint Pathology Center | And 3 more authors.
Toxicologic Pathology | Year: 2013

A 12-week-old Swiss Albino mouse was presented with unilateral (left) testicular enlargement of approximately 1.5 cm in diameter and the right testicle mildly reduced in size and weight. Histopathology evaluation revealed three distinct neoplasms in the left testicle: choriocarcinoma, yolk sac carcinoma, and embryonal carcinoma. Teratoma was diagnosed in the right testicle. The histomorphological and immunohistochemical characteristics of the tumor are presented here. To the best of the authors' knowledge, this is the first report of spontaneous nonmetastasizing choriocarcinoma, yolk sac carcinoma, embryonal carcinoma, and teratoma in testes of a Swiss albino mouse. © 2013 by The Author(s).

PubMed | National Research Institute of Ayurvedic Drug Development and Hospital Division
Type: | Journal: Journal of diabetes and metabolic disorders | Year: 2014

Dyslipidemia is one of the major risk factors for cardiovascular disease in diabetes mellitus. Early detection and treatment of dyslipidemia in type-2 diabetes mellitus can prevent risk for atherogenic cardiovascular disorder. The rationale of this study was to detect the lipid abnormality in diabetic patients.Necessary data was collected from the medical archives of 150 patients (73 female and 77 male) with diabetes mellitus registered in Department of pathology and biochemistry of a Ayurveda hospital established at Kolkata, India.The mean ages of female and male subjects were 51.810.8 and 53.211.3years respectively. The range and mean value of FBS in females were 113-342mg/dl and 157.76.3mg/dl, while the range and mean value of PPBS in females were 135-560mg/dl and 275.512.3mg/dl respectively. Results showed that range and mean value of FBS in males were 111-462mg/dl and 160.87.4mg/dl, while the range and mean value of PPBS in males were 136-598mg/dl and 302.112.6mg/dl respectively. Results of serum lipids showed that the mean values for total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) in female patients were 202.25.9mg/dl, 168.38.2mg/dl, 44.91.3mg/dl, 123.65.2mg/dl and 33.71.7mg/dl respectively. The mean values for TC, TG, HDL-C, LDL-C and VLDL-C in male patients were 182.54.8mg/dl, 128.110.8mg/dl, 40.81.2mg/dl, 105.44.8mg/dl and 36.22.2 respectively. FBS showed significant positive correlation with PPBS, cholesterol, TG, and VLDL-C. PPBS also demonstrated direct and significant correlations with TG and VLDL-C.The study showed common lipid abnormalities during diabetes induced dyslipidemia i.e., hypercholesterolemia, hypertriglyceridemia and elevated LDL-C. This study suggests the dominance of hyperlipidemia over increased prevalence of dyslipidemia.

PubMed | National Research Institute of Ayurvedic Drug Development
Type: Journal Article | Journal: Ayu | Year: 2014

Haritaki (Terminalia chebula Retz., Family: Combretaceae) possesses a great therapeutic value and is widely distributed in India, up to an altitude of 1500 m. Though Terminalia chebula Retz is the only botanical source of Haritaki, the uses of its varieties along with their sources, identifying features and therapeutic uses are described in Ayurvedic classics and other medical literature. In the present study, a detailed review has been carried out on different varieties of Haritaki.

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