National Research Institute for Forestry

Celaya, Mexico

National Research Institute for Forestry

Celaya, Mexico
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De La Mora-Orozco C.,National Research Institute for Forestry | Flores-Lopez H.,National Research Institute for Forestry | Rubio-Arias H.,Autonomous University of Chihuahua | Chavez-Duran A.,National Research Institute for Forestry | Ochoa-Rivero J.,National Research Institute on Forestry
International Journal of Environmental Research and Public Health | Year: 2017

Pollution levels have been increasing in water ecosystems worldwide. A water quality index (WQI) is an available tool to approximate the quality of water and facilitate the work of decision-makers by grouping and analyzing numerous parameters with a single numerical classification system. The objective of this study was to develop a WQI for a dam used for irrigation of about 5000 ha of agricultural land. The dam, La Vega, is located in Teuchitlan, Jalisco, Mexico. Seven sites were selected for water sampling and samples were collected in March, June, July, September, and December 2014 in an initial effort to develop a WQI for the dam. The WQI methodology, which was recommended by the Mexican National Water Commission (CNA), was used. The parameters employed to calculate the WQI were pH, electrical conductivity (EC), dissolved oxygen (DO), total dissolved solids (TDS), total hardness (TH), alkalinity (Alk), total phosphorous (TP), Cl–, NO3, SO4, Ca, Mg, K, B, As, Cu, and Zn. No significant differences in WQI values were found among the seven sampling sites along the dam. However, seasonal differences in WQI were noted. In March and June, water quality was categorized as poor. By July and September, water quality was classified as medium to good. Quality then decreased, and by December water quality was classified as medium to poor. In conclusion, water treatment must be applied before waters from La Vega dam reservoir can be used for irrigation or other purposes. It is recommended that the water quality at La Vega dam is continually monitored for several years in order to confirm the findings of this short-term study. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

Feregrino-Perez A.A.,Autonomous University of Queretaro | Pinol-Felis C.,Hospital Arnau Of Vilanova | Gomez-Arbones X.,Hospital Arnau Of Vilanova | Guevara-Gonzalez R.G.,Autonomous University of Queretaro | And 3 more authors.
Plant Foods for Human Nutrition | Year: 2014

We have previously demonstrated that the non-digestible fraction (NDF) from common cooked beans (P. vulgaris L., cv Negro 8025) inhibits azoxymethane (AOM)-induced colon cancer and influences the expression of genes involved in the induction of apoptosis and cell cycle arrest through the action of butyrate. The objective of this study was to identify cell cycle alterations and morphological changes induced by treatment with AOM and to examine the formation of colonic aberrant crypt foci (ACF) in male Sprague Dawley rats fed with these beans. Rats were fed control diets upon arrival and were randomly placed into four groups after one week of acclimatization: control, NDF (intragastric administration), NDF + AOM and AOM. Rats treated with NDF + AOM exhibited a significantly lower number of total colonic ACF with a notable increase in the number of cells present in the G1 phase (83.14 %); a decreased proliferation index was observed in the NDF + AOM group when compared to AOM group. NDF + AOM also displayed a higher number of apoptotic cells compared to AOM group. NDF of cooked common beans inhibited colon carcinogenesis at an early stage by inducing cell cycle arrest of colon cells and morphological changes linked to apoptosis, thus confirming previous results obtained with gene expression studies. © 2014 Springer Science+Business Media New York.

Vergara-Castaneda H.A.,Autonomous University of Queretaro | Guevara-Gonzalez R.G.,Autonomous University of Queretaro | Ramos-Gomez M.,Autonomous University of Queretaro | Reynoso-Camacho R.,Autonomous University of Queretaro | And 4 more authors.
Food and Function | Year: 2010

The non-digestible fraction (NDF) of common bean (Phaseolus vulgaris L.) cultivar Bayo Madero was evaluated for its chemopreventive effect on azoxymethane (AOM) induced aberrant crypt foci (ACF) in rats. Diets containing cooked beans (CB) or its non-digestible fraction (NDF) were fed to 72 male rats after 2 azoxymethane injections (15 mg kg-1 of body weight once a week for 2 weeks). ACF number, short chain fatty acids (SCFA) and β-glucuronidase activity were measured in colon sections from rats sacrificed 7 weeks after the last AOM injection. Food intake and weight gain of rats were unaffected by CB and NDF. CB and NDF suppressed the AOM-induced formation of ACF (0.8 and 1.5 ACF/distal zone, respectively vs. 6.6 ACF/distal zone based on methylene blue stain) and lowered β-glucuronidase activity in cecal, colonic and fecal content compared to AOM group. SCFA production was not significantly different among fecal, cecal and colonic content. These results indicate that CB and NDF from Bayo Madero provide direct chemoprotection against early stage of azoxymethane (AOM)-induced colon cancer in rats. © The Royal Society of Chemistry.

Rubio-Arias H.,Autonomous University of Chihuahua | Contreras-Caraveo M.,Autonomous University of Chihuahua | Quintana R.M.,Autonomous University of Chihuahua | Saucedo-Teran R.A.,National Research Institute for Forestry | Pinales-Munguia A.,Autonomous University of Chihuahua
International Journal of Environmental Research and Public Health | Year: 2012

A Water Quality Index (WQI) is a useful statistical tool for simplifying, reporting and interpreting complex information obtained from any body of water. A simple number given by any WQI model explains the level of water contamination. The objective was to develop a WQI for the water of the Luis L. Leon dam located in the state of Chihuahua, Mexico. Monthly water samples were obtained in 2009; January 10, February 12, March 8, May 20, June 10, July 9, August 12, September 10, October 11, November 15 and December 13. Ten sampling sites were randomly selected after dividing the study area using a geographic package. In each site, two samples at the top depth of 0.20 m and 1.0 m were obtained to quantify physical-chemical parameters. The following 11 parameters were considered to calculate the WQI; pH, Electrical Conductivity (EC), Dissolved Oxygen (DO), color, turbidity, ammonia nitrogen, fluorides, chlorides, sulfates, Total Solids (TS) and phosphorous (P). The data analysis involved two steps; a single analysis for each parameter and the WQI calculation. The resulted WQI value classified the water quality according to the following ranges: <2.3 poor water; from 2.3 to 2.8 good water; and >2.8 excellent water. The results showed that the WQI values changed from low levels (WQI < 2.3) in some points during autumn time to high levels (WQI > 2.8) most of the year and the variation was due to time of sampling generally rainy season. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Flores-Castro R.,National Research Institute for Forestry | Loza-Rubio E.,National Research Institute for Forestry
Avian Diseases | Year: 2010

Specific-pathogen-free chickens immunized at 14 days of age with either an inactivated recombinant Newcastle disease virusLaSota/avian influenza H5 (K-rNDV-LS/AI-H5) vaccine or a killed Newcastle disease/avian influenza whole-virus vaccine (K-ND/AI) were protected from disease when challenged with either A/chicken/Queretaro/14588-19/95 (H5N2), a high pathogenicity avian influenza virus (HPAIV) strain isolated in Mexico in 1995, or with a Mexican velogenic viscerotropic Newcastle disease virus (VVNDV) strain 21 days postvaccination. All nonvaccinated chickens challenged with HPAIV or VVNDV succumbed to disease, while those vaccinated with K-rNDV-LS/AI-H5 or K-ND/AI were protected from severe clinical signs and death. Both vaccines induced hemagglutination-inhibition (HI) antibody responses against NDV and AIV. Antibodies against AIV nucleoprotein were not detected by enzyme-linked immunosorbent assay (ELISA) in birds vaccinated with the inactivated rNDV-LS/AI-H5 vaccine. These chickens became positive for AIV antibodies by ELISA only after challenge with HPAIV. The data clearly indicate that the inactivated rNDV-LS/AI-H5 vaccine confers protection comparable to that of the conventional killed whole-virus vaccine against both NDV and AIV, while still allowing differentiation of infected from vaccinated animals by HI and ELISA tests. © 2010 American Association of Avian Pathologists.

The protective dose of a live recombinant LaSota Newcastle disease virus (NDV)-avian influenza H5 vaccine (rNDV-LS/AI-H5) was determined in broiler chickens with high levels of maternal antibodies against NDV and avian influenza virus (AIV). At hatch the geometric mean titers (GMT) of the chickens' maternal antibodies were 25.1 and 210.3 for NDV and AIV, respectively. At the time of vaccination the GMT was 23.1 for NDV and 27.9 for AIV. The chickens were vaccinated with one drop (0.03 ml) in the eye at 10 days of age as is typical under field conditions. The test chickens received 104.8, 105.8, 106.8, or 107.8 mean chicken embryo infective doses (CEID50) of the rNDV-LS/AI-H5 vaccine. Control chickens were either nonvaccinated, or vaccinated with 105.8 or 106.8 CEID50 of a commercial live LaSota NDV vaccine. Birds were challenged with either the Mexican highly pathogenic avian influenza virus (HPAIV) strain A/Chicken/Queretaro/14588-19/95 (H5N2) or a Mexican velogenic viscerotropic (VV) NDV strain. One hundred percent of the chickens vaccinated with the rNDV-LS/AI-H5 vaccine were protected against HPAIV and VVNDV when a challenge dose of 106.8 EID 50 or higher was administered by eye drop. Birds vaccinated with the LaSota NDV vaccine were protected against VVNDV, but not against HPAIV. © 2010 American Association of Avian Pathologists.

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