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Teixeira da Silva J.A.,Kagawa University | Rana T.S.,CSIR - Central Electrochemical Research Institute | Narzary D.,Gauhati University | Verma N.,National Bureau of Plant Genetic Resources NBPGR | And 2 more authors.
Scientia Horticulturae | Year: 2013

Pomegranate (Punica granatum L.) is one of the oldest known edible fruit tree species, originating in Central Asia, but with a wide geographical global distribution, reflecting its adaptation to a wide range of climatic conditions. It is important for its nutritional, medicinal and ornamental properties and its high consumption and industrial value. In a bid to better utilize and improve the current genetic resources, there is a need to understand and appreciate studies related to the use, centre of origin and diversity, as well as the characterization, evaluation and conservation, taxonomy and systematics of the genus Punica. In addition to understanding the basic biology of the plant, how biotechnological tools, including cell and tissue culture and micropropagation (i.e. somatic embryogenesis, organogenesis, synthetic seeds, somaclonal variation, mutagenesis, haploidy, and in vitro conservation), genetic transformation and marker technology, have been used to improve pomegranate germplasm are all topics that have been covered in this review. © 2013 Elsevier B.V. Source

Singh N.V.,National Research Center on Pomegranate | Singh S.K.,Indian Agricultural Research Institute | Singh A.K.,Indian Agricultural Research Institute
Vitis - Journal of Grapevine Research | Year: 2011

In-ovulo embryo rescue in grape breeding programme assures breeding efficiency by curtailing 6 to 8 years in the development of seedless grape cultivars. Effect of different growth regulators, culture media, ovule age, cultivars and mycorrhizal strains on successful in-ovulo embryo rescue were studied in this experiment. With respect to mean ovule age (days after pollination), maximum ovule-embryo growth (2.13 mm 2) were obtained when ovules were cultured at 24 days after pollination (DAP) but maximum germination (12.67%) was obtained when ovules were cultured at 28 DAP. The concentration of IAA (4 mg) + GA3 (0.5 mg) proved to be most effective for germination (13.84%). Among various media used for culturing ovules on modified MS medium (1/2 macro +1/1 micro) required minimum days to germinate (96.67) and registered highest germination (13.75%). Chilling treatment was proved to be one of the important factors for embryo maturation and 60 d of chilling treatment at 4 °C improved embryo germination. With respect to the effect of different growth regulators on various rooting and shooting parameters IBA (1.0 mg L -1) and NAA (1.5 mg-L -1) were found superior. While comparing different hardening strategies, a glass jar with polypropylene cap (GPP) was found to be most effective as far as hardening success (66.67%) was concerned. One of the serious impediments in the success of in vitro rescued plantlets is very high field mortality, field survival of rescued plantlets can be effectively increased by using different mycorrhizal strains (AMF) as bio-hardening agents. Among the various strains used for hardening of rescued plantlets T3 inoculated plantlets registered highest survival percent (88.00). Source

Marathe R.A.,National Research Center on Pomegranate | Dhinesh Babu K.,National Research Center on Pomegranate
Indian Journal of Horticulture | Year: 2015

For precise diagnosis of leaf nutrient content, it is essential to determine the season of sampling and position of leaves. A field trial was conducted to standardize the leaf sampling technique for nutrient diagnosis in pomegranate cv. Bhagwa grown under semi-arid tropical climate of Solapur, Maharashtra. The results revealed that the seasonal variation in leaf nutrient content was higher and most unstable during rainy season followed by winter and summer season. Monthly analysis of leaf samples showed that leaf N (0.70 to 0.99%), K (1.08 to 1.14%) and Ca (1.41 to 1.58%) content stabilized during November to June, April to June and February to June month respectively. Leaf P content remained more or less constant while Fe and Cu showed maximum fluctuation throughout the year. There was gradual reduction in P (0.195 to 0.121%), Zn (30.6 to 21.4 ppm) and increase in Ca (1.40 to 1.79%) content up to 10th leaf pair from growing tip. It is ideal to collect 10th leaf pair from growing tip during the month of February to March for nutrient diagnosis in the plant. © 2015, Horticulture Society of India. All rights reserved. Source

Chandra R.,National Research Center on Pomegranate | Jadhav V.T.,National Research Center on Pomegranate
Indian Journal of Agricultural Sciences | Year: 2012

Pomegranate (Punica granatum L.) is one of the important fruit crops grown on commercial scale in Deccan Plateau of India and is gaining a lot of popularity worldwide in recent years owing to its high economic, nutraceutical and therapeutic values (Marathe et al. 2010). It is mainly propagated by air layering in Maharashtra, Karnataka and Andhra Pradesh. Unlike other perennial fruit crops, multistem training system is very common in pomegranate (Chandra et al. 2008). Recently, wilt has emerged as an important threat in major pomegranate growing belts of India and to combat this problem neither any standard grafting technique nor suitable rootstock is available. In fruit crops, wedge graft specifically offers very high graft success with greater stability than the other grafting techniques tried due to the instant full balance between the stock and the scion (Tabora and Atienza 2006, Selvi et al. 2008, Somkumar et al. 2009). But, this method was not tested in pomegranate especially for commercial cultivars. Therefore, an attempt was made to standardize grafting method and time in Bhagawa cultivar of pomegranate. Source

Jadhav V.T.,National Research Center on Pomegranate | Sharma K.K.,National Research Center on Pomegranate
Acta Horticulturae | Year: 2011

Bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae (Hingorani and Singh) Dye has become an increasingly serious threat to pomegranate growers in the states of Karnataka, Maharashtra and Andhra Pradesh in India. The present investigation was carried out during 2005 and 2006 respectively and revealed that sprays with streptocycline (500 ppm) + copper oxychloride (2000 ppm) were found very effective in reducing the mean disease incidence (25.5%) followed by Bromopal (500 ppm) + copper oxychloride (2000 ppm) (33.3%), when compared with control (78.5%) after the 8 th spray. The maximum mean yield of 9.3 tons/ha was recorded in streptocycline (500 ppm) + copper oxychloride (2000 ppm) followed by 8.50 tons/ha in Bromopal (500 ppm) + copper oxychloride (2000 ppm). The untreated check yielded 2.95 tons/ha. Source

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