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Gadekar Y.P.,Bombay Veterinary College | Kokane R.D.,Bombay Veterinary College | Suradkar U.S.,Bombay Veterinary College | Thomas R.,National Research Center on Pig | And 2 more authors.
International Food Research Journal

Meat is a nutrient dense food. But due to its perishable nature, meat and meat products require considerable input for chilling or freezing during storage and marketing. Meat provides a suitable environment for proliferation of meat spoilage microorganisms and common food-borne pathogens, therefore adequate preservation technologies must be applied in order to preserve its safety and quality. Preservation of meat either in fresh or in processed form requires considerable energy. High perishability of meat and meat products is serious problem in tropical countries. Now days there is need to develop the foods which are shelf-stable so that can be distributed in various locations without aid of refrigeration. Pickling is one of the alternatives to develop such kind of products. © 2008 IFRJ. Source

Naskar S.,National Research Center on Pig | Deb S.M.,Indian Central Arid Zone Research Institute | Niranjan S.K.,National Bureau of Animal Genetic Resources | Kumar S.,Indian Veterinary Research Institute | And 2 more authors.
Genetics and Molecular Biology

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β 1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A. © 2012, Sociedade Brasileira de Genética. Source

Magotra A.,University of Veterinary and Animal Sciences | Naskar S.,National Research Center on Pig | Das B.,Assam Agricultural University | Ahmad T.,NDRI
Molecular Biology Reports

The Follicle stimulating hormone beta polypeptide (FSHβ) gene has been investigated as a candidate gene for litter size because of its role in maturation of small and medium follicles into ovulating large follicles. Genes coding follicle stimulating hormone have been found to have significant effects on litter size in pigs. The aim of the study was to investigate the polymorphism of the first intron of FSH-β gene present in Ghungroo, Niang Megha and Nagaland local pigs of India. PCR based short interspersed nuclear elements (SINE) detection method and PCR–RFLP were carried out to identify the polymorphism in FSHβ gene. Significantly higher frequencies of SINE−/− and SINE+/− genotypes were observed in Ghungroo pigs as compared to Niang Megha and Nagaland local pigs. PCR–RFLP study also showed higher frequency of T allele in all the three breeds even though there existed difference in genotypes among breeds. Association studies clearly demonstrated that SINE−/− genotypes have significantly higher litter size and weight at birth as well as at weaning. SINE −/− genotypes have higher number of live births too. Moderately high D′ value indicates that haplotypic pattern within the two loci can be utilized for marker assisted selection for litter traits in pigs. © 2014, Springer Science+Business Media Dordrecht. Source

Barman J.,National Research Center on Pig | Baruah U.K.,National Research Center on Pig | Goswami U.C.,Gauhati University
Indian Journal of Traditional Knowledge

Fishing techniques for catching the mud eel, Monopterus cuchia (Ham.) in Goalpara district, Assam was investigated during 2007-10 during the course of a base line survey conducted by the KVK Goalpara, Assam. The complexities of its habitat, behavior and life history characteristics, makes it difficult to catch the fish. Documentation on the fishing methods for catching the mud eel is scanty. This paper is an attempt to record the different technique employed traditionally by the ethnic communities of the district to catch the mud eel based on the information collected during the course of survey. The results revealed use of different wounding gears, ichthyotoxic plants, handline and traps. Wounding gears included spear, knives and sickle. Among plants, Derris elliptica (Wall.) Benth. and Milletia pachycarpa Benth. were used. Spindle shaped and cubical traps are commonly used by the common folks. Other method includes bunding and digging, and light fishing. Source

Ranjan Sahoo N.,National Research Center on Pig | Nesa N.,Indian Veterinary Research Institute | Naskar S.,Research Fellow | Banik S.,Manipur University | Pankaj P.K.,ICAR Central Research Institute for Dry Land Agriculture
Indian Journal of Animal Sciences

An attempt was made to measure the genetic variation available within Ghoongroo pigs, a prized germplasm in terms of high growth and multiplication rate among the indigenous varieties, using 22 FAO-ISAG microsatellite markers. The data were analyzed applying different software to estimate the various measures of genetic diversity. The average number of observed allele was 8.18±0.62 and the effective average number of allele was 4.39±0.26. The observed heterozygosity ranged from 0.4 to 0.96 and the expected heterozygosity from 0.55 to 0.85. Average PIC value ranged from 0.55 (S0026) to 0.85 (S0068) with the average value 0.75±0.02. The mean F value was 0.07±0.04. There was no genetic bottleneck observed in the studied population. A total of 45 private alleles were observed which can be used as breed specific markers. The results suggested that all the microsatellite markers were highly polymorphic and suitable for molecular characterization of Ghoongroo pig. There was substantial genetic variation and polymorphism across the studied loci. The low inbreeding observed was a favourable parameter to formulate the appropriate breeding strategies to enhance heterozygosity in the population. Source

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