National Research Center for Plant Biotechnology

Delhi, India

National Research Center for Plant Biotechnology

Delhi, India

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Revathi P.,Indian Agricultural Research Institute | Revathi P.,National Research Center for Plant Biotechnology | Tomar S.M.S.,Indian Agricultural Research Institute | Vinod,Indian Agricultural Research Institute
Indian Journal of Genetics and Plant Breeding | Year: 2010

Two highly effective genes for leaf rust resistance viz., Lr24, Lr28 and a stripe rust resistance gene Yr15 were selected for pyramiding in the background of a susceptible but high yielding bread wheat variety HD2877. The screening against most virulent pathotypes of leaf rust 77-5 (121R63-1) and stripe rust, 46S119 and 78S84 indicated that all the three genes confer a high degree of seedling and adult plant resistance. The use of molecular markers, namely, SCS1302 607, SCS421 570 and Xgwm273 validated the presence of resistance genes, Lr24 and Yr15 in Sunstar* 6/C80-1//V763- 2312 and Lr28 in HW2033 both being donors. The application of molecular markers facilitated identification of individual plants in three-way cross (HD2877 × Sunstar* 6/C80-1//V763-2312) × HW2033, BC 1-F 1 and BC 2-F 1 generations possessing the targeted genes. Finally eight plants were selected in BC2-F2 generation carrying the desired resistance genes, Lr24, Lr28 and Yr15 in different combinations in the background of HD2877. The availability of combination of major rust resistance genes in desirable background would facilitate the strategic deployment of wheat varieties to achieve durable resistance.


Manjulatha M.,Sri Krishnadevaraya University | Manjulatha M.,South Korean National Institute of Crop Science | Sreevathsa R.,University of Agricultural Sciences, Bangalore | Sreevathsa R.,National Research Center for Plant Biotechnology | And 5 more authors.
Molecular Biotechnology | Year: 2014

Peanut, a major edible oil seed crop globally is predominantly grown under rainfed conditions and suffers yield losses due to drought. Development of drought-tolerant varieties through transgenic technology is a valid approach. Besides superior water relation traits like water mining, intrinsic cellular level tolerance mechanisms are important to sustain the growth under stress. To achieve this objective, the focus of this study was to pyramid drought adaptive traits by overexpressing a stress responsive helicase, PDH45 in the background of a genotype with superior water relations. PCR, Southern, and RT-PCR analyses confirmed stable integration and expression of the PDH45 gene in peanut transgenics. At the end of T3 generation, eight transgenic events were identified as promising based on stress tolerance and improvement in productivity. Several transgenic lines showed stay-green phenotype and increased chlorophyll stability under stress and reduced chlorophyll retardation under etherel-induced simulated stress conditions. Stress-induced root growth was also substantially higher in the case of transformants. This was reflected in increased WUE (low Δ13C) and improved growth rates and productivity. The transgenics showed 17.2 and 26.75% increase in yield under non-stress and stress conditions over wild type ascertaining the feasibility of trait pyramiding strategy for the development of drought-tolerant peanut. © Springer Science+Business Media 2013.


Pandey A.K.,Indian Agricultural Research Institute | Chaudhary P.,Indian Agricultural Research Institute | Singh S.B.,Indian Agricultural Research Institute | Arora A.,Indian Agricultural Research Institute | And 3 more authors.
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering | Year: 2012

Polycyclic aromatic hydrocarbons (PAHs) are wide spread industrial pollutants that are released into the environment from burning of coal, distillation of wood, operation of gas works, oil refineries, vehicular emission, and combustion process. In this study a lipolytic bacterium was isolated from mixed stover compost of Saccharum munja and Brassica campestris. This strain was identified by both classical and 16S ribosomal DNA sequencing method and designated as Serratia marcesencs L-11. HPLC-based quantitation revealed 39- 100% degradation of PAH compounds within seven days. Further its ability to produce catechol 1, 2-dioxygenase (1.118 μM mL -1 h -1) and biosurfactants (0.88 g L -1) during growth in PAH containing medium may be responsible for its PAH-degradation potential. This novel bacterium with an ability to produce lipases, biosurfactant and ring cleavage enzyme can prove to be useful for in-situ degradation of PAH compounds. © 2012 Copyright Taylor and Francis Group, LLC.


Das S.,Indian Council of Agricultural Research | Krishnan P.,Indian Council of Agricultural Research | Mishra V.,National Research Center for Plant Biotechnology | Kumar R.,Indian Council of Agricultural Research | And 2 more authors.
Molecular Biology Reports | Year: 2015

The interactive effect of temperature with other climatic and soil factors has profound influences on the growth and development of rice. The responses of rice to high temperatures under field conditions are more important than those under the controlled conditions. To understand the genes associated with high temperature stress response in general and tolerance in particular, the expression of all those genes associated with adaptation and tolerance in rice requires proteomic analysis. High temperature stress-tolerant cv. N22 was subjected to 28/18 °C (control) and 42/32 °C (high temperature stress) at flowering stage. The plants were grown in the field under the free air temperature increment condition. The proteomic changes in rice leaves due to high temperature stress were discussed. The proteomes of leaves had about 3000 protein spots, reproducibly detected on 2-dimensional electrophoretic gels with 573 proteins differentially expressed between the control and the high temperature treatments. Putative physiological functions suggested five categories such as growth (15.4 %), heat shock proteins (7.7 %), regulatory proteins (26.9 %), redox homeostasis proteins (11.5 %) and energy and metabolism (38.5 %) related proteins. The results of the present study suggest that cv. N22, an agronomically recognized temperature tolerant rice cultivar copes with high temperature stress in a complex manner. Several functional proteins play important roles in its responses. The predicted climate change events necessitate more studies using this cultivar under different simulated ecological conditions to identify proteomic changes and the associated genes to be used as biomarkers and to gain a better understanding on the biochemical pathways involved in tolerance. © 2015, Springer Science+Business Media Dordrecht.


Singh S.,Indian Agricultural Research Institute | Singh S.,Central Agricultural Research Institute | Sharma S.R.,Indian Agricultural Research Institute | Kalia P.,Indian Agricultural Research Institute | And 4 more authors.
Journal of Horticultural Science and Biotechnology | Year: 2012

The present study aimed to construct a linkage map for the downy mildew resistance gene, Ppa3, in cauliflower. Ppa3 is a dominant, single locus gene in cauliflower which confers downy mildew resistance. Out of 190 markers screened, 13 were found to be polymorphic between the two parental lines, 'BR-2' and 'Pusa Himjyoti'. Among these 13 polymorphic markers, six were random amplified polymorphic DNA (RAPD) markers (OPB18 1276, OPB20 1174, OPC14 1186, OPC1 850, OPF1 960, and OPE14 1881) and the remaining seven were inter-simple sequence repeat (ISSR) markers (ISSR5 525, ISSR8 1125, ISSR11 550, ISSR12 1050, ISSR23 1103, ISSR30 575, and ISSR35 620). Furthermore, bulked segregant analysis identified only three markers (OPC14 1186, OPE14 1881, and ISSR23 1103) as being polymorphic. An F 2 mapping population consisting of 120 plants was analysed using the latter three polymorphic markers and a linkage map was constructed for Ppa3. OPC14 1186 was located 22.3 cM from the Ppa3 gene on the top-arm end, with OPE14 1881 and ISSR23 1103 at 10.6 cM and 26.4 cM, respectively on the other side of the Ppa3 gene on the linkage map. This information may be useful for further studies to identify more closely-linked markers for the Ppa3 gene.


Namita,Indian Agricultural Research Institute | Panwar S.,Indian Agricultural Research Institute | Sonah H.,Indian Agricultural Research Institute | Singh K.P.,Indian Agricultural Research Institute | And 2 more authors.
Indian Journal of Agricultural Sciences | Year: 2013

The genetic diversity of 15 genotypes of Tagetes erecta L. (African marigold; Af/ws-1, Af/ws-2, Af/ws-3, Af/ws-4, Af/ws-5, Af/ws-6 and Af/ws-7) and Tagetes patula L. (French marigold; Fr/ws-1, Fr/ws-2, Fr/ws-3, Fr/ws-4, Fr/ws-5, Fr/ ws-6, Fr/ws-7 and Fr/ws-8) were assessed using PCR based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat) markers. Twenty three RAPD primers were used to generate polymorphism and mean per cent polymorphism was found as 57.72% ranging from 33.33% (OPF-2) to 83.33% (RAPD-07). Five most informative primers (OPA-02, OPA-04, OPO-10, RAPD-07 and RAPD-18) were identified on the basis of level of polymorphism detected by individual primer. Twelve ISSR primers were also used to generate polymorphism and mean per cent polymorphism was 60.48% ranging from 40.00% (ISSR-02, ISSR-17) to 90.00% (ISSR-03). The five most informative primers (ISSR-03, ISSR-06, ISSR-12, ISSR-31 and ISSR-18) were also identified on the basis of polymorphism. The dendrogram obtained from UPGMA cluster analysis of Jaccard's similarity values based on 23 RAPD and 12 ISSR markers grouped 15 genotypes into two clusters species wise (Tagetes erecta and Tagetes patula) which confirm the reliability of PCR based molecular markers. PCA analysis also confirmed the grouping of genotypes as they were present in same group as revealed by RAPD and ISSR markers.


PubMed | Indian Council of Agricultural Research, National Research Center for Plant Biotechnology and Indian Agricultural Research Institute
Type: Journal Article | Journal: Molecular biology reports | Year: 2015

The interactive effect of temperature with other climatic and soil factors has profound influences on the growth and development of rice. The responses of rice to high temperatures under field conditions are more important than those under the controlled conditions. To understand the genes associated with high temperature stress response in general and tolerance in particular, the expression of all those genes associated with adaptation and tolerance in rice requires proteomic analysis. High temperature stress-tolerant cv. N22 was subjected to 28/18 C (control) and 42/32 C (high temperature stress) at flowering stage. The plants were grown in the field under the free air temperature increment condition. The proteomic changes in rice leaves due to high temperature stress were discussed. The proteomes of leaves had about 3000 protein spots, reproducibly detected on 2-dimensional electrophoretic gels with 573 proteins differentially expressed between the control and the high temperature treatments. Putative physiological functions suggested five categories such as growth (15.4%), heat shock proteins (7.7%), regulatory proteins (26.9%), redox homeostasis proteins (11.5%) and energy and metabolism (38.5%) related proteins. The results of the present study suggest that cv. N22, an agronomically recognized temperature tolerant rice cultivar copes with high temperature stress in a complex manner. Several functional proteins play important roles in its responses. The predicted climate change events necessitate more studies using this cultivar under different simulated ecological conditions to identify proteomic changes and the associated genes to be used as biomarkers and to gain a better understanding on the biochemical pathways involved in tolerance.


Krishnamurthy S.L.,Indian Central Soil Salinity Research Institute | Sharma S.K.,Indian Central Soil Salinity Research Institute | Kumar V.,Indian Central Soil Salinity Research Institute | Tiwari S.,National Research Center for Plant Biotechnology | Singh N.K.,National Research Center for Plant Biotechnology
Journal of Plant Biochemistry and Biotechnology | Year: 2016

Micro satellite markers located in the Saltol QTL of 5 Mb region (10.4–15.6 Mb) in chromosome 1 confering seedling stage salt tolerance were used to evaluate 94 rice genotypes. Out of 21, eight SSR markers at Saltol region of Chromosome were found polymorphic. Based on the phenotypic screening, 94 genotypes were grouped as highly tolerant (20), tolerant (18) moderately tolerant (32), sensitive (19) and highly sensitive (5). The marker RM3412 appears to be diagnostic of salinity tolerance and associate to salinity tolerance at seedling stage as it is closely linked to SKC gene. Based on Saltol markers study, CSR 31, CSR 38, CSR 41, CSR 32, Wild 11, CSR 18, Azgo, Pant Dhan 4, Trichi 1, CSR 10 and IR64426-4B-11-1 could not be identified as tolerant genotypes though had expressed tolerant to highly tolerant phenotype to salinity stress at seedling stage, suggesting that QTLs other than Saltol might be controlling their salinity tolerance. It is suggested that these genotypes could serve as potentially novel germplasm and could be exploited for the development of new breeding lines with high level of salinity tolerance by pyramiding of the Saltol and other QTLs. © 2015, Society for Plant Biochemistry and Biotechnology.


Padaria J.C.,National Research Center for Plant Biotechnology | Thuy N.T.,National Research Center for Plant Biotechnology | Tarafdar A.,National Research Center for Plant Biotechnology | Yadav R.,National Research Center for Plant Biotechnology
Journal of Horticultural Science and Biotechnology | Year: 2015

High temperature adversely affects the growth, yield, and quality of crops. Prosopis cineraria, an indigenous plant in India, is highly tolerant of environmental stresses. In the present study, an expressed sequence tag (EST) library was constructed using pooled RNAs from 1-month-old P. cineraria seedlings subjected to heat stress at 48ºC for between 15 min to 48 h. A total of 84 unigenes (20 contigs and 64 singletons) were generated from the cluster assembly of 132 good ESTs. Of these, nine unigenes did not show any homology to sequences in the NCBI database. A BLASTX comparison of the remaining 75 unigenes revealed significant similarities to known genes. The 84 ESTs were classified into 14 functional categories, of which 13.75% were in the stress-response category, including approx. 10% that were for heat-shock proteins. The heat stress-induced ESTs reported here are the first for P. cineraria. A phylogenetic analysis based on the complete coding sequence of the Pchsp17.9 gene showed a close relationship to hsp17.9 of Acacia mangium. Levels of expression of genes related to hsp chaperones were analysed by reverse transcriptionquantitative PCR (RT-qPCR) in 1-month-old heat-stressed seedlings of P. cineraria and were found to be highly upregulated. © 2015, Headley Brothers Ltd. All rights reserved.


Nadella K.D.,National Research Center for Plant Biotechnology | Marla S.S.,National Bureau of Plant Genetic Resources | Kumar P.A.,National Research Center for Plant Biotechnology
OMICS A Journal of Integrative Biology | Year: 2012

Metabolome refers to the complete set of metabolites synthesized through a series of multiple enzymatic steps from various biochemical pathways processing the information encrypted in the plant genome. Knowledge about synthesis and regulation of various plant metabolic substances has improved substantially with availability of Omics data originating from sequencing of plant genomes. Metabolic profiling of crops is increasingly becoming popular in assessing plant phenotypes and genetic diversity. Metabolic compositional changes vividly reflect the changes occurring during plant growth, development, and in response to stress. Hence, study of plant metabolic pathways, the interconnections between them in context of systems biology is increasingly becoming popular in identification of candidate genes. The present article reviews recent developments in analysis of plant metabolomics, available bioinformatics techniques and databases employed for comparative pathway analysis, metabolic QTLs, and their application in plants. © Copyright 2012, Mary Ann Liebert, Inc.

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