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Bhat K.V.,National Research Center for Fingerprinting | Singh S.K.,Indian Agricultural Research Institute
Indian Journal of Genetics and Plant Breeding | Year: 2010

The present investigation was undertaken for characterizing tuberose genotypes using DNA marker technology. Twenty tuberose genotypes comprising of both single- and double-petal types collected from different parts of India were selected for analysis. The DNAv extraction and RAPD conditions were standardized. For RAPD analysis 20 ng DNA template, 2.5 mM MgCl2 and 1U TaqDNA polymerase was found effective. Out of 80 random decamer primers tested, 17 were selected based on high level of polymorphism. On the basis of primer resolving power and marker index, RAPD primers OPC-13 and OPD- 12 were identified as efficient primers for diversity analysis of tuberose. A total of 157 RAPD bands were generated by the 17 random decamer primers. The selected primers proved effective for DNA profiling in addition to diversity analysis. Genotypes Guwahati Double and Swarnrekha showed good morphological similarity revealing high similarity coefficient (0.90) suggesting them to be very closely related.The RAPD analysis also confirmed their relatedness as they grouped in the same cluster. The suitability of this technique for genotyping and diversity analysis was also established. Genotypes, Vaibhav and Pune Single were found to have least pair-wise similarity although they had a greater morphological similarity with each other.


Bantawa P.,North Bengal Agricultural University | da Silva J.A.T.,Kagawa University | Ghosh S.K.,North Bengal Agricultural University | Mondal T.K.,North Bengal Agricultural University | Mondal T.K.,National Research Center for Fingerprinting
Journal of Horticultural Science and Biotechnology | Year: 2011

Leaves of mature of Gaultheria fragrantissima Wall. plants (Indian wintergreen) were collected from various locations in the Eastern Himalayan region on the Indo-China border and were analysed by steam distillation and gas chromatography to identify an elite line that contained 1.79% (v/v) essential oils, 98% of which was methyl salicylate. Subsequently, a highly reproducible micropropagation protocol using adult shoot tips from this elite genotype was developed in order to conserve this highly-valued, endangered, woody oil-bearing aromatic shrub in India. Among several plant growth regulator (PGR) combinations tested for in vitro multiplication, up to 35 shoots per explant could be induced within 14 weeks of culture on woody plant medium (WPM) fortified only with 0.22 mg l -1 thidiazuron (TDZ). These shoots could elongate on WPM containing 1.0 mg l -1 kinetin. Rooted plantlets were acclimatised ex vivo, with 70% success. Random amplified polymorphic DNA (RAPD) analysis indicated the genetic uniformity of both the micropropagated plantlets and the donor plants. This is the first report on in vitro micropropagation of G. fragrantissima.


Sud S.,Bhabha Atomic Research Center | Bains N.S.,Punjab Agricultural University | Nanda G.S.,Punjab Agricultural University | Singh K.,Punjab Agricultural University | Arya L.,National Research Center for fingerprinting
Sabrao Journal of Breeding and Genetics | Year: 2010

This study was conducted to find the relationship between heterosis and genetic diversity in wheat by using molecular, morphological and pedigree data. Twenty elite parental lines were crossed in all possible combinations. The F1 hybrids and parents were evaluated for agronomic performance in replicated yield trial. The parental lines were assayed for DNA polymorphism using 60 wheat microsatellite primers and 12 AFLP (EcoR1: Mse1) primers. Pedigree information at expansion level of 5 was collected and co-efficient of parentage (COP) values were calculated. Eighteen germplasm descriptors were used to estimate morphological diversity. The range of genetic diversity was highest for morphological (0.22 to 0.83) followed by pedigree (0.47 to 1.00), simple sequence repeat markers (0.09 to 0.53) and amplified fragment polymorphic markers (0.13 to 0.46). Genetic diversity estimates using SSRs (GDSSRs) was positively correlated (r = 0.283) with pedigree-based diversity. The correlation among other diversity measures was found to be low and non significant. GD SSRs and GD COPs were the only diversity measure to show association with yield heterosis as well as heterosis for yield components (thousand grain weight). Significant moderate correlations were also observed between GDAFLP and plant height and GDMOR with grain yield per spike.


Singh R.K.,National Research Center for Soybean | Singh R.K.,Indian Agricultural Research Institute | Bhatia V.S.,National Research Center for Soybean | Bhat K.V.,National Research Center for Fingerprinting | And 4 more authors.
Genetics and Molecular Biology | Year: 2010

Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly moderate bootstrap support. These major clusters corresponded with the photoperiod response and place of origin. The results indicate that the photoperiod insensitive genotypes, 11/2/1939 (EC 325097) and MACS 330 would be better choice for broadening the genetic base of soybean for this trait. © 2010, Sociedade Brasileira de Genética.


Dutta S.K.,Indian Agricultural Research Institute | Srivastav M.,Indian Agricultural Research Institute | Chaudhary R.,Tissue Culture and Cryopreservation Unit | Lal K.,Indian Agricultural Research Institute | And 3 more authors.
Scientia Horticulturae | Year: 2013

A study was conducted to investigate pollen viability of three polleniser mango cultivars, viz. 'Sensation', 'Tommy Atkins' and 'Janardan Pasand' up to 24 weeks under four storage conditions (room temperature, -4. °C, -20. °C and -196. °C). Three methods of pollen viability testing, viz. in vitro germination, fluorescein diacetate (FDA) and acetocarmine staining were used. Storage methods and interaction between storage methods and days of storage had highly significant effect on pollen viability (p≤. 0.0001). Room temperature storage of pollen in the three mango cultivars showed very low pollen viability after 4 weeks of storage, after which pollens were not viable. Irrespective of mango genotypes, cryo-stored (-196. °C) pollens showed significantly higher viability as compared to all the other storage conditions. The differential results obtained by using different pollen viability assay confirmed that in vitro germination test was more reliable compared to FDA or acetocarmine tests, where germination was often overestimated. From the present study, we suggest storage of pollen at -20. °C for pollination among cultivars having non-synchronized flowering in a season. However, for long term storage cryo method proved to be the best for pollen storage in mango. © 2013 Elsevier B.V.


Singh R.K.,Sugarcane Research Institute | Singh S.P.,Sugarcane Research Institute | Tiwari D.K.,Sugarcane Research Institute | Srivastava S.,Sugarcane Research Institute | And 5 more authors.
Euphytica | Year: 2013

Genetic improvement of sugar content in sugarcane would benefit from the availability of sufficient DNA markers and a genetic map. Genetic linkage maps were constructed to identify quantitative trait loci (QTLs) for seedling brix (SB), brix (B), sucrose percent in juice (SUC), stalk number (SN), stalk length (SL), stalk diameter (SD), internodes (INT), number of green leaves (NGL), at three crop cycles across seven environments in a segregating population with 207 individuals derived from a bi-parental cross of sugarcane elite cultivars. Linkage analysis led to the construction of eight linkage groups (LGs) for Co86011 and sixteen LGs for CoH70. The combined length of the two linkage maps was 2606.77 cM distributed over 24 LGs. 31 QTLs were identified: 2 for SB, 7 for B, 6 for SUC, 4 for SN, 1 for SL, 3 for SD, 6 for INT and 2 for NGL at LOD scores ranging from 2.69 to 4.75. 7 QTLs (22 %) had stable effect across crop year and locations. Markers from parents were found to be associated with both positive and negative effect on all of the traits analyzed. The most important QTLs intervals identified in this study using single-dose marker, were qB2, qSUC2, qINT2 and qB2, qSUC2, qSL2, qINT2 located between SSR markers UGSM31548 and UGSM31649. These QTLs could be put into use in marker assisted breeding. © 2012 Springer Science+Business Media Dordrecht.


Ganie S.A.,Visva Bharati | Ganie S.A.,National Research Center for Fingerprinting | Karmakar J.,Visva Bharati | Roychowdhury R.,Visva Bharati | And 2 more authors.
Plant Systematics and Evolution | Year: 2014

A heterogeneous collection of rice genotypes which included seven salt-tolerant rice lines, one salt-sensitive improved line, one wild rice (Oryza rufipogon) and one salt-tolerant wild rice relative (Porteresia coarctata) was screened with ten salt-tolerance-linked simple sequence repeat markers, of which nine were from the Saltol QTL mapped on rice 1st chromosome and the rest one from 8th chromosome, having high phenotypic variance for salt tolerance. Variation in molecular weight (in the form of base pairs) of the different amplified products using RM primers was used to find out the genetic relationship among the studied rice genotypes. Genomic DNA of the studied genotypes was also amplified with a reported allele mining primer for a salt-inducible gene (salT). The amplified products were sequenced and aligned to find out the closeness among the rice lines for the studied gene. Dendrogram derived from marker profiles showed partial similarity with salT gene-derived tree. Commonly, all the salt-tolerant lines were grouped into a single cluster, including IR36 (a salt-sensitive line) to which O. rufipogon (the wild rice) and P. coarctata (the wild rice relative) joined separately. The taxonomic identity and evolutionary relationship among the three groups (rice, wild rice and wild rice relative) were bioinformatically analysed using the nucleotide sequence of the studied salT gene. © 2014 Springer-Verlag Wien.

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