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Buciumeanu E.-C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Guta I.C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Bejan C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Topala C.M.,University of Pitesti
Revista de Chimie | Year: 2014

In vitro cultures of V. vinifera L, cv. Feteascə neagrə 7 Od. infected with grapevine fanleaf virus (GFLV) have been investigated on the influence of chemotherapeutics ribavirin and oseltamivir, added in medium, in order to block viral replication and virus-free plants regeneration. The concentrations of nitrogen, carbon, sulphur and hydrogen in grapevine plantlets were dosed, as nutrients having clear role in plant growth and development. The viricides determined a significant increasing of nitrogen in plantlets as compared to the control, after 60 and 90 days of treatment, while the carbon concentration decreased. The sulphur and hydrogen concentrations were not significantly influenced by the mixture of chemotherapeutics. Despite these modifications, the micropropagation processes continued until new grapevine plants regeneration. Therefore, ribavirin and oseltamivir could be used with the aim of grapevine healthy plant obtaining. Source


Guta I.C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Buciumeanu E.-C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Gheorghe R.N.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Teodorescu A.,University of Pitesti
Romanian Biotechnological Letters | Year: 2010

The use of electrotherapy in continuous electric field and also in vitro chemotherapy with ribavirin and oseltamivir were investigated as solutions to eliminate grapevine leafroll associated virus serotype 1+3 from V. vinifera L., cv. Ranâi Magaraci. The efficacy of virus elimination process was assessed by enzyme-linked immunosorbent assay testing during the in vitro stages of experiments and at their end, on regenerated acclimated grapevine plants. The results showed no virus eradication in ribavirin (120 μmol L-1) treated apices. Oseltamivir (50 μmol L-1) administration led to obtain virus-free plants in the percentage of 66.66-71.42%. The electrotherapy followed by in vitro culture of shoot apices produced 33.33- 66.66% virus -free plants, regardless explant position of exposed plant in continuous electric field. In order to test the genetic stability, fidelity and uniformity of the plants obtained after electrotherapy followed by in vitro culture the random amplified polymorphic technique was applied. The patterns generated by all primers proved to be monomorphic across all the tested plants. © 2010 University of Bucharest. Source


Popescu C.F.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Dejeu L.C.,University of Agronomical Sciences and Veterinary Medicine | Bejan C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges
Vitis - Journal of Grapevine Research | Year: 2015

The paper presents an up-to-date description of some autochthonous, old and valuable grapevine varieties maintained within the germplasm collection belonging to the University of Agronomical Science and Veterinary Medicine Bucharest, respectively 3 varieties for red wines ('FeteascǍ neagrǍ', 'BǍbeascǍ neagrǍ', 'BusuioacǍ de Bohotin') and 6 varieties for white wines ('FeteascǍ albǍ', 'FeteascǍ regalǍ', 'GalbenǍ de Odobeşti', 'GrasǍ de Cotnari', 'TǍmâioasǍ româneascǍ' and 'ZghiharǍ de Huşi'). The results obtained by applying standardized methodology for grape quantitative and qualitative parameters for three consecutive years, reveal the main characteristics for each variety and also the specific genotype response to particular pedoclimatic conditions. In comparison to 'Cabernet Sauvignon' as reference cultivar, 'BǍbeascǍ neagrǍ' and 'BusuioacǍ de Bohotin' varieties were characterized by higher values for the sizes and weights of the berries, and 'FeteascǍ neagrǍ' by heavier bunches and higher sugar juice content. The white grape varieties in comparison to 'Chardonnay' as reference cultivar, were remarked either by bigger bunches with high to very high sugar content ('FeteascǍ regalǍ', 'GrasǍ de Cotnari', 'TǍmâioasǍ româneascǍ'), or by constancy of bunch weight and titratable acidity of must ('FeteascǍ albǍ', 'GalbenǍ de Odobeşti' and 'ZghiharǍ de Huşi'). Source


Buciumeanu E.-C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Visoiu E.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Guta I.C.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges | Popescu C.F.,National Research and Development Institute for Biotechnology in Horticulture Stefanesti Arges
Romanian Biotechnological Letters | Year: 2010

One of the most important mission of the National Research and Development Institute for Biotechnology in Horticulture Ştefǎneşti-Argeş is to establish the initial grapevine plant-material collection and to preserve it in proper conditions. The infected cultivars and clones detected as positive for virus infection were subjected to virus elimination through heat treatment and/or in vitro culture by adapting the working protocols, especially the duration of the treatment, to the particularities of each virus. Starting from 1993, ELISA is the most used method both for diagnosis and studies regarding the sampling strategy for different viruses (detection of the most reliable source of antigen and period of the year in which the analyze is performed). In order to use the virus infected biologic material as reference (positive controls) in our studies, a grapevine virus infected collection was established. The behaviour of the infected grapevine material in comparison to the healthy one, both in vivo and in vitro conditions, have been investigated. The studies of cellular modifications in the presence of virus infection emphasized the usefulness of ultrastructural changes both for understanding the biological answer of the plants under stress conditions and as a valuable aid in virus diagnosis purposes. © 2010 University of Bucharest. Source

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