Entity

Time filter

Source Type


Giovannozzi A.M.,INRIM - Istituto Nazionale di Ricerca Metrologica | Rolle F.,INRIM - Istituto Nazionale di Ricerca Metrologica | Sega M.,INRIM - Istituto Nazionale di Ricerca Metrologica | Abete M.C.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | And 2 more authors.
Food Chemistry | Year: 2014

A rapid and sensitive method to detect melamine in liquid milk based on Surface Enhanced Raman Scattering (SERS) spectroscopy is presented, exploiting the selective binding of gold nanoparticles (AuNPs) with this analyte. This interaction promotes the aggregation of the AuNPs inducing a huge enhancement of the melamine signals in the Raman spectrum due to the formation of SERS "hot spots". An external standard calibration method was employed for quantitative analysis and the method was validated for linearity, sensitivity, repeatability and recovery. A good linearity (R2 = 0.99) was found in the concentration range of 0.31-5.0 mg l-1 in milk with a limit of detection of 0.17 mg l-1. This method does not require a long extraction procedure (total analysis time can be lower than 30 min) and can be reliably used for melamine detection in milk matrix in accordance with the European law limits. © 2014 Elsevier Ltd. All rights reserved. Source


Benedetto A.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Squadrone S.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Prearo M.,Fish Diseases Laboratory | Elia A.C.,University of Perugia | And 2 more authors.
Chemosphere | Year: 2011

Gene expression experiments were targeted in order to monitor the ABC efflux transporters, which is potentially involved in cellular detoxification/defense. Changes in expression levels of different ABC genes in kidney of Oncorhynchus mykiss fed with melamine and melamine+cyanuric acid enriched diets were recorded in both treated groups by mRNA ΔΔCT relative quantification method. Expression profiles of eight different ABC genes basically showed low alterations in melamine group and more consistent changes in melamine+cyanuric acid treated fish, compared with own control. In the last group ABCC2 gene over expression was the more evident alteration. These results suggest that ABC efflux system could be involved in mobilization of hydrophilic molecules in the forcing condition of chronic exposure. © 2011 Elsevier Ltd. Source


Benedetto A.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Abete M.C.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Squadrone S.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed
Food Chemistry | Year: 2011

A real-time PCR method to detect fish DNA in feedstuffs was developed and optimised. A combination of primers and a Taqman-MGB probe was used to selectively amplify the fish mitochondrial 12S ribosomal RNA gene. Qualitative and also quantitative assessments were performed with different protocols: a relative quantification by a standard curve, and a ΔC T method, by total plant DNA as endogenous controls. Method specificity was evaluated analysing 40 different tissues (mammalians, avian, fish) and flour samples. Sensitivity was evaluated by LOD (limit of detection) estimation. The designed probe-primers set showed an increased sensitivity compared to previously published PCR end point method, reaching a limit of detection of 0.2 pg of fish DNA, and showing to be a robust assay for fish DNA detection. The quantification results, based on ΔC T method and the relative standard curve, are well reproducible in our experimental condition but, in lacking of separate pure raw materials of a tested feed, they cannot be applied for reliable and precise quantification on field samples but for now as a semi-quantitative PCR method only. © 2010 Elsevier Ltd. All rights reserved. Source


Squadrone S.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Marchis D.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Loria A.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | Amato G.,Creaa National Reference Center For The Surveillance And Monitoring Of Animal Feed | And 2 more authors.
Food Control | Year: 2015

An enzyme-linked immunosorbent assay (ELISA) test has been applied for the qualitative screening analysis of virginiamycin and bacitracin in feedingstuffs at level of 1mg/kg. The ELISA validation study was performed according to the Commission Decision 2002/657/EC criteria established for qualitative screening methods in food. In this regard, the following parameters were determined: detection capability (CCβ), specificity and ruggedness. The resulted CCβ values were <1ppm for both molecules and no interferences from matrix effects were observed. Slight variations of some critical factors in the sample pre-treatment and clean up were deliberately introduced for ruggedness evaluation and they did not result in any negative effect on the detection of virginiamycin, while ruggedness evaluation on the detection of bacitracin showed some critical factors. The proposed method is suitable for qualitative screening analysis of virginiamycin and bacitracin in conformity with the current EC performance requirements. © 2014 Elsevier Ltd. Source

Discover hidden collaborations