Vasile E.,Science METAV CD S.A. |
Popescu L.M.,Petru Poni Institute of Macromolecular Chemistry |
Popescu L.M.,National Rand D Institute for Non Ferrous and Rare Metals |
Piticescu R.M.,National Rand D Institute for Non Ferrous and Rare Metals |
And 2 more authors.
The present paper aims to evaluate the physico-chemical and biocompatible properties of some new nanostructured hydroxyapatite-polyurethane (HAP-PU) composite scaffolds in direct contact with MSCs by in vitro tests. Several HAP based nanostructures were obtained by hydrothermal synthesis in various pressure conditions, i.e. pressure varying between 20-80 atm and temperature less than 100 0 C. For the first time, a polyurethane with 100% hard segment content with carboxyl functional groups was used to prepare ceramic-polymer composites (80% HAP) with potential applications in bone tissue engineering. HAP crystallites with 15-35 nm in length were obtained. The results of MSCs viability indicated a slightly higher viability of HAP coatings (72% vs. control) compared to HAP-PU composites (69% vs. control) synthesized at high pressure (60 atm). This could be due to the effect of particle shape on cellular viability. © 2012 Elsevier B.V. All rights reserved. Source