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Oancea F.,National R and D Institute for Chemistry and Petrochemistry ICECHIM | Szabolcs L.,Sapientia University | Oancea A.-O.,Romanian National Institute for Research and Development for Biological Sciences | Lacatusu R.,National Research and Development Institute for Soil Science | And 4 more authors.
Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii | Year: 2014

The low level of selenium in soils from different Romanian areas indicates a need for its supplementation. Biofortification through treatments applied to crops has the advantage of supplementation of food chain with controlled levels of selenium compounds, with high bioavailability. We present a biotechnological approach of biofortification, adapted to the conditions specific for the soils from South-Eastern part of Romania, which is based on the use of selected plant growth promoting rhizobacteria (PGPR). We evaluated, in controlled experiments, the ability of selected PGPR to increase, in a reference South-East Romania soil, the selenium bioavailability for wheat plant. We integrate this selenium biofortification biotechnology into a GIS-based decision support system. We are discussing Se biofortification in relation with its interferences with polyamines metabolism. We are evaluating the potential use of a new emerging biotechnological approach, based on plant tissue nitric oxide inductors, for a more equilibrated selenium compounds accumulation. © 2014 Vasile Goldis University Press. Source

Voicescu M.,Institute of Physical Chemistry Ilie Murgulescu | Nistor C.L.,National R and D Institute for Chemistry and Petrochemistry ICECHIM | Meghea A.,Polytechnic University of Bucharest
Journal of Luminescence | Year: 2015

The work aims to simulate in vitro the effects caused by oxidation of five hydroxyflavones (HF) (some typical models of flavonols), (3-HF, 6-HF, 7-HF, 3,6-diHF and 3,7-diHF) on silver nanoparticles (SNPs) using the chemiluminescent system luminol-hydrogen peroxide, in phosphate buffer, pH 7.4. The contribution of bovine and human serum albumins to the antioxidant activity of the mentioned flavones, and the effect on the SNPs support, in the chemiluminescent system luminol-hydrogen peroxide, has been also investigated. The results are discussed with relevance to the oxidative stress process. © 2014 Elsevier B.V. Source

Zagorodko O.,Lille University of Science and Technology | Bouckaert J.,Lille University of Science and Technology | Dumych T.,Lille University of Science and Technology | Bilyy R.,Ukrainian Academy of Sciences | And 10 more authors.
Biosensors | Year: 2015

The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-D-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 μL·min-1), and reaches a maximum at flow rates of 30 μL·min-1 (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces. © 2015 by the authors. Source

Bunghez I.-R.,National R and D Institute for Chemistry and Petrochemistry ICECHIM | Dumitrescu O.,Polytechnic University of Bucharest | Somoghi R.,National R and D Institute for Chemistry and Petrochemistry ICECHIM | Ionita I.,University Valahia of Targoviste | And 2 more authors.
Revista de Chimie | Year: 2015

The fruits of mulberry (Morus nigra) are used in herbal therapies due to their tonic and depurative properties. They are used to treat lung and throat diseases, thrush, diabetes, gastric and duodenal ulcers. Some research studies have revealed high content of beneficial acids (phenolic, ascorbic - vitamin C), antioxidant and antiinflammatory properties of mulberry fruits. In the present paper, we have developed a simple green and economical method for synthesis of silver nanoparticles (AgNPs) using this type of fruits. The analytical techniques (UV-Vis, SEM, DLS and FTIR) studies suggested that the mulberry fruits have played an important role in the reduction and stabilization of silver nanoparticles. Using UV-Vis method, it was observed an intense peak at 465 nm specific for silver nanoparticles. SEM revealed the formation of spherical nanoparticles with size less than 40 nm, after ultrasonication. DLS method was used to determine the size of particles and FTIR spectra indicate the appearance of peaks in the amide I and II regions (characteristic to proteins), which have been found to be possible responsible for capping and efficient stabilization of the metal nanoparticles synthesized by the mulberry fruit. The eco friendly silver nanoparticles presented strong antioxidant properties, so AgNP-mulberry sample (AA%=93.23) has a higher activity than mulberry extract sample (AA%=88.25). Source

Barbinta-Patrascu M.E.,University of Bucharest | Bunghez I.-R.,National R and D Institute for Chemistry and Petrochemistry ICECHIM | Bunghez I.-R.,University Valahia of Targoviste | Iordache S.M.,University of Bucharest | And 4 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2013

This paper is aimed to describe a simple and rapid eco-friendly bottom-up approach for the preparation of antioxidant silver bionanostructures using a leaf extract from sage (Salvia officinalis L.). The bioreduction property of sage in the synthesis of silver nanoparticles was investigated by UV-VIS and Attenuated Total Reflectance Fourier Transform Infrared spectroscopy. During their preparation, the particle size analysis was performed by using Dynamic Light Scattering technique. Ultrasonic irradiation was used to obtain sage silver nanoparticles. The morphology (size and shape) of the herbal silver nanoparticles was evaluated by Scanning Electron Microscopy that revealed the formation of spherical phytonanoparticles with size less than 80 nm. In order to increase their stability and their biocompatibility, the sage silver nanoparticles were introduced in two types of liposomes: soybean lecithin- and Chla-DPPC-lipid vesicles which were prepared by thin film hydration method. X-Ray Fluorescence analysis confirmed the silver presence in liposomes/sage-AgNPs biohybrids. The stability of liposomes/herbal AgNPs bioconstructs was checked by zeta potential measurements. The most stable biohybrids: Chla-DPPC/sage-AgNPs with zeta potential value of -34.2 mV, were characterized by Atomic Force Microscopy revealing the spherical and quasi-spherical shaped profiles of these nanobiohybrids with size less than 96 nm. The antioxidant activity of the silver bionanostructures was evaluated using chemiluminescence assay. The developed eco-friendly silver phytonanostructures based on lipid membranes, nanosilver and sage extract, manifest strong antioxidant properties (between 86.5% and 98.6%).Copyright © 2013 American Scientific Publishers All rights reserved. Source

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