Chirvase A.A.,National Research and Development Institute for Chemistry and Petroleum Chemistry |
Ungureanu C.,Polytechnic University of Bucharest |
Tcacenco L.,Romanian National Institute for Research and Development for Biological Sciences |
Radu N.,National Research and Development Institute for Chemistry and Petroleum Chemistry
Revista de Chimie | Year: 2010
Recently, enzymatic transesterification using lipase has become more attractive for biodiesel fuel production, since the glycerol produced as a by-product can be easily recovered and the purification of the fatty methyl esters is simple to accomplish. Preliminary research work done on testing several yeasts strains as lipase producers demonstrated the most promising were the yeasts Yarrowia lipolytica ATCC 8661 and Candida rugosa DSM 70761. The paper presents the results of their complete testing to investigate the conditions of lipase formation and the obtained enzymes capacity to catalyze the vegetable oils transesterification to biodiesel The research methodology comprises: design of cultivation medium and bioprocessing conditions; determination of yeast specific growth rate and lipase activity; isolation of extracellular lipase by centrifugation and ammonium sulfate precipitation; crude enzyme testing in transesterification process.