Rajakumaran P.,Alagappa University |
Vaseeharan B.,Alagappa University |
Jayakumar R.,Central Marine Fisheries Research Institute CMFRI |
Chidambara R.,National Prawn Co
Cytology and Genetics | Year: 2014
Understanding of accurate phylogenetic relationship among Penaeidae shrimp is important for academic and fisheries industry. The Morphometric and Randomly Amplified Polymorphic DNA (RAPD) analysis was used to make the phylogenetic relationsip among 13 Penaeidae shrimp. For morphometric analysis forty variables and total lengths of shrimp were measured for each species, and removed the effect of size variation. The size normalized values obtained was subjected to UPGMA (Unweighted Pair-Group Method with Arithmetic Mean) cluster analysis. For RAPD analysis, the four primers showed reliable differentiation between species, and used correlation coefficient between the DNA banding patterns of 13 Penaeidae species to construct UPGMA dendrogram. Phylogenetic relationship from morphometric and molecular analysis for Penaeidae species found to be congruent. We concluded that as the results from morphometry investigations concur with molecular one, phylogenetic relationship obtained for the studied Penaeidae are considered to be reliable. © 2014, Allerton Press, Inc.
Al-Adali K.,National Prawn Company |
Ahmed E.,National Prawn Company |
Kumar P.,National Prawn Company |
Ayaril N.,National Prawn Company
Journal of King Abdulaziz University, Marine Science | Year: 2012
Experiments were conducted to determine optimal salinity, temperature, nutrient level and CO2 supply required for achieving maximal growth in culture of two commercially important microalgae Nannochloropsis gaditana L.M. Lubián and Dunaliella salina (Dunal) Teodoresco. Four different salinities, temperatures and nutrient levels (NaNO3) and two different CO2 levels were employed. The results indicate that maximum cell density was obtained in N. gaditana with cultures in 25 ppt salinity, 25°C temperature, 150 g/lit nutrient level and 0.5 scfm CO2 supply. In the case of D. salina the highest cell density was with 150 ppt salinity, 30°C temperature, 150 g/lit nutrient level and 2 scfm CO2 supply. Second set of experiments with a combination of optimal parameters resulted in significant increase in cell density with both algae compared to routine culture conditions.