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Wageningen, Netherlands

Roenhorst J.W.,National Plant Protection Organization | Boonham N.,UK Environment Agency | Winter S.,Julius Kuhn Institute | Menzel W.,Leibniz Institute DSMZ | van der Vlugt R.A.A.,Plant Research International
EPPO Bulletin

The availability of characterised reference isolates of plant pathogens is of crucial importance for research and diagnostic laboratories. The Q-bank Plant Viruses and Viroids database contains data and information on plant viruses and viroids, with the unique feature that it is linked to specimens present in publicly available physical collections. The Q-bank database aims to share data and information on the virus and viroid species and their availability between research and diagnostic laboratories. Currently the database focuses on regulated virus species. In future more plant viruses and viroids will be included to provide a comprehensive data and information system. The curators invite virologists worldwide to participate in this international initiative by making their data and isolates available via Q-bank (http://www.q-bank.eu). © 2013 The Authors. Journal compilation © 2013 OEPP/EPPO. Source

Bakker D.,Naktuinbouw | Bruinsma M.,Naktuinbouw | Dekter R.W.,Naktuinbouw | Toonen M.A.J.,Naktuinbouw | And 2 more authors.
EPPO Bulletin

Worldwide outbreaks of pospiviroids in potato and tomato have increased the need for a reliable test for the detection of pospiviroids in seeds. This study describes the development and validation of a sensitive and fast test for the detection of Potato spindle tuber viroid (PSTVd) and Tomato chlorotic dwarf viroid (TCDVd) in tomato seeds. The test is based on RNA isolation using a commercial kit and is suitable for routine application. The test is able to detect one PSTVd or TCDVd contaminated seed in sub samples of 1000 seeds and results were both repeatable and reproducible. © 2015 OEPP/EPPO. Source

Alvani S.,Ferdowsi University of Mashhad | Mahdikhani-Moghadam E.,Ferdowsi University of Mashhad | Rouhani H.,Ferdowsi University of Mashhad | Mohammadi A.,Birjand University | Karssen G.,National Plant Protection Organization

In order to identify plant-parasitic nematodes (family Tylenchidae Örley 1880) associated with Ziziphus zizyphus in Iran, 360 soil and root samples were collected from South Khorasan province during 2012-2014. Herein, a new species of Basiria and several known members of the family Tylenchidae are reported. B. birjandiensis n. sp. is characterized by short body length (584-748 μm [660.6±72.3]), lip region with flat apex, stylet 11-12 μm (11.3±0.5), excretory pore position varying from isthmus level to the middle of the basal bulb (78-91 μm from the anterior end of the body), post-vulval uterine sac 8-14 μm (10.7±1.9) long, filiform tail (151-181 μm, c= 3.7-4.2, c= 14.3-17.2) and body annuli 0.5-1 μm (0.6±0.1) wide. A checklist of Tylenchidae species from Iran is also presented. © Copyright 2016 Magnolia Press. Source

Dullemans A.M.,Wageningen University | Verhoeven J.T.J.,National Plant Protection Organization | Kormelink R.,Wageningen University | van der Vlugt R.A.A.,Wageningen University
Archives of Virology

The complete genome sequence of chrysanthemum stem necrosis virus (CSNV) was determined using Roche 454 next-generation sequencing. CSNV is a tentative member of the genus Tospovirus within the family Bunyaviridae, whose members are arthropod-borne. This is the first report of the entire RNA genome sequence of a CSNV isolate. The large RNA of CSNV is 8955 nucleotides (nt) in size and contains a single open reading frame of 8625 nt in the antisense arrangement, coding for the putative RNA-dependent RNA polymerase (L protein) of 2874 aa with a predicted Mr of 331 kDa. Two untranslated regions of 397 and 33 nt are present at the 5’ and 3’ termini, respectively. The medium (M) and small (S) RNAs are 4830 and 2947 nt in size, respectively, and show 99 % identity to the corresponding genomic segments of previously partially characterized CSNV genomes. Protein sequences for the precursor of the Gn/Gc proteins, N and NSs, are identical in length in all of the analysed CSNV isolates. © 2014, Springer-Verlag Wien. Source

News Article | March 7, 2016
Site: http://phys.org/biology-news/

The polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. Scientists of Ghent University (Belgium), the National Plant Protection Organization (the Netherlands) en het International Institute of Tropical Agriculture (Nigeria) refined the identification of the nematodes.

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