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Matsushita K.,Japan National Agricultural Research Center | Yasuda N.,Japan National Agricultural Research Center | Thinlay,National Plant Protection Center | Koizumi S.,Japan National Agricultural Research Center | And 6 more authors.
Euphytica | Year: 2011

The rice cultivar 'Chumroo' is commonly cultivated in the mid- and high-altitude areas of Bhutan. This cultivar has shown durable blast resistance in that area, without evidence of breakdown, for over 20 years. Chumroo was inoculated with 22 blast isolates selected from the race differential standard set of Japan. The cultivar showed resistance to all the isolates. To identify the resistance gene(s), Chumroo was crossed with a susceptible rice cultivar, Koshihikari. The F1 plants of the cross showed resistance. Segregation analyses of 300 F3 family lines fitted the segregation ratio of 1:2:1 and indicated that a single dominant gene controls the resistance to a blast isolate Ao 92-06-2 (race 337. 1). The Chumroo resistance locus (termed Pi46(t)) was mapped between two SSR markers, RM6748 and RM5473, on the terminal region of the long arm of chromosome 4, using linkage analysis with SSR markers. The nearest marker, RM5473, was linked to the putative resistance locus at a map distance of 3. 2 cM. At the chromosomal region, no true resistance genes were identified, whereas two field resistance genes were present. Therefore, we designated Pi46(t) as a novel blast resistance locus. © 2011 Springer Science+Business Media B.V.

Holford P.,University of Western Sydney | Donovan N.J.,Elizabeth Macarthur Agricultural Institute | Thinlay,National Plant Protection Center | Kabanoff E.,University of Western Sydney | And 6 more authors.
Australasian Plant Disease Notes | Year: 2010

Powdery mildew is one of the most important diseases of citrus in Bhutan where it infects new flush growth causing leaf and shoot distortion and twig and branch dieback. It also attacks young fruitlets. This causes premature fruit drop. These symptoms, together with DNA sequence data and the production of single conidia and lobed appressoria, suggest that the disease is caused by Oidium citri (JM Yen) U. Braun. This is the first formal report of this pathogen in Bhutan. © Australasian Plant Pathology ociety 2010.

Donovan N.J.,Australian Department of Primary Industries and Fisheries | Beattie G.A.C.,University of Western Sydney | Chambers G.A.,Australian Department of Primary Industries and Fisheries | Holford P.,University of Western Sydney | And 4 more authors.
Australasian Plant Disease Notes | Year: 2012

Huanglongbing (HLB) or citrus greening is one of the most destructive diseases of citrus in the world and one of the major factors limiting citrus production in south east Asia including Bhutan. The presence of 'Candidatus Liberibacter asiaticus', associated with the Asiatic form of HLB, was confirmed by conventional and real-time PCR in adults of the black psyllid, Diaphorina communis Mathur. This is the first formal detection of 'Ca. L. asiaticus' in D. communis, and the first detection of the pathogen in a psyllid other than D. citri Kuwayama in Asia, excluding Arabia. This study is also the first to report the presence of D. communis in Bhutan. © 2011 Australasian Plant Pathology Society Inc.

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