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Zamani M.J.,Iranian Seed and Plant Improvement Institute | Solouki M.,University of Zabol | Zahravi M.,National Plant Gene Bank of Iran | Imamjomeh A.A.,University of Zabol | And 2 more authors.
Molecular Biology Reports | Year: 2010

Aegilops tauschii Coss. is a diploid (2n = 2x = 14,DD) goat grass species which has contributed the D genome in common wheat. Genetic variations in 28 accessions of Aegilops tauschii belonged to different provinces of Iran, were evaluated using 16 morphological traits and 19 SSR markers. In number of spikelet per spike and plant height, there was a high variation in ssp. tauschii and ssp. strangulata respectively and for days to mature a low variation in both subspecies was found. Discriminant function analysis showed that 67.9% of original grouped cases correctly classified. Factor analysis indicated that three factor explain 66.49% of total variation. The three clusters revealed by the cluster analysis were not consistent with their geographical distributions. We determined 208 alleles using 19 microsatellites. Average of alleles for every locus was 10.94. The total average of PIC was 0.267. 2261 bands produced for total of genotypes and Chinese Spring had the highest bands (95 alleles). The range of similarity coefficients was between 0.23 and 0.73. Genotypes were clustered using UPGMA method. The accessions did not match according to morphological cluster and geographical regions. 51.2% of total variations were related to 9 principle components. © 2009 Springer Science+Business Media B.V. Source

Moslemi M.,Islamic Azad University at Tehran | Zahravi M.,National Plant Gene Bank of Iran | Khaniki G.B.,Payame Noor University
Scientia Horticulturae | Year: 2010

Pomegranate (Punica granatum L.) is one of the oldest known edible fruits. It is native to Iran and spread from Iran to other areas. In this study amplified fragment length polymorphism (AFLP) was used to detect intra- and inter-population genetic diversity of pomegranate. A group of 67 accessions belonged to 4 populations from Iran was studied using eight primer combinations. A total of 221 scorable bands were amplified, of which, 118 (54.13%) were polymorphic. Resolving power (Rp) ranged from 5.70 to 9.21, and the average of polymorphism information content (PIC) per primer pair was 0.40. According to Nei's gene diversity and allelic statistics, Isfahan population had a highest genetic diversity (H=0.3646, I=0.5327, Ne=1.6467). Coefficient of gene differentiation between populations (GST) was 0.124, indicated that mainly proportion of genetic variation (87.6%), was within populations and the remaining (12.4%) of the variation was among populations that, also supported by analysis of molecular variance (AMOVA). The gene flow (Nm) varied from 0.969 to 10.404 between pair-wise populations and was 3.504 among all of the populations. The Jaccard similarity coefficient between individuals ranged from 0.26 to 0.88. The UPGMA dendrogram clustered all 67 accessions into 6 groups. In some cases accessions from same region were grouped together but in most cases, there was gene exchange. To study the genetic relationships among populations, a principal coordinate analysis (PCoA) based on Nei's genetic distances was performed. Results of this study showed that AFLP marker can be a useful tool for investigating the genetic diversity of pomegranate genotypes. © 2010 Elsevier B.V. Source

Beiki A.H.,University of Zanjan | Keifi F.,University of Zanjan | Mozafari J.,National Plant Gene Bank of Iran
Genetic Engineering and Biotechnology Journal | Year: 2010

Saffron has for decades been the world's most expensive spice by weight. It is a spice derived from the flower of the saffron (Crocus sativus), a species of Crocus in the family Iridaceae. Considering the importance of germplasm characterization and evaluation particularly in saffron, the present study was formulated with an objective to access the structure of genetic diversity among the accession of saffron at molecular levels. Twenty four wild type and six cultivated colonies were gathered from seven different states and were evaluated by 26 random amplified polymorphic DNA (RAPD) molecular markers that we were expecting, to assess degree of genetic variability between them. A total of 322 bands were scored corresponding to an average of 16.6 bands per primer with 281 bands showing polymorphism (87.3%) and values of polymorphism information content (PIC) varying from 0.24 to 0.72. Results obtained after use of RAPD markers showed that "EM1-EM5" is more accurate and its application is recommended. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster I comprised four cancellatuse species. Cluster-II comprises of 26 cultivars that were further differentiated into two sub-clusters. All genotypes in subgroup IIa include six cultivated ones. The PCoA revealed a pattern in which the individuals were assigned into five separate groups. Cluster analysis and 2D and 3D principal component analysis demonstrated the effectiveness of RAPD markers in grouping of accessions under study, and analysis of their genetic relationships. Source

Tahernezhad Z.,Seed and Plant Certification and Registration Institute | Musavi Z.,University of Zabol | Zamani M.J.,Iranian Seed and Plant Improvement Institute | Jafar Aghaei M.,National Plant Gene Bank of Iran | Rostam Foroudi B.,Iranian Seed and Plant Improvement Institute
Genetic Resources and Crop Evolution | Year: 2013

Variation of high molecular weight glutenin subunits (HMW-GS) in 28 Iranian Aegilops tauschii (2n = 2x = 14, DD) accessions studied by sodium dodecyl sulphate electrophoresis method (SDS-PAGE). The results showed high variation of HMW-GS in the accessions. The range of frequency in 14 HMW-GS combinations was 3. 57-25 % in the accessions. AMOVA showed the molecular variance between the geographic areas was lower than within the geographic areas. According to Nei's genetic diversity, the highest diversity levels were in Semnan, Golestan and Azarbayjan, on the other hand the lowest levels of diversity were found in Khorasan, Gilan and Mazandaran accessions. Hence, the Caspian Sea South East accessions also Azerbayjan in Iran have more diversity. AMOVA did not show variance between strangulata and tauschii but there was more genetic diversity in ssp. tauschii subspecies in comparison of ssp. strangulata according to Nei's gene diversity and Shannon information index. It showed Iranian Ae. tauschii have a good potential for bread making quality improvement in bread wheat. © 2012 Springer Science+Business Media Dordrecht. Source

alamuti M.K.,Agricultural Biotechnology Research Institute of Iran | Zeinalabedini M.,Agricultural Biotechnology Research Institute of Iran | Derazmahalleh M.M.,Agricultural Biotechnology Research Institute of Iran | RoodbarShojaie T.,Agricultural Biotechnology Research Institute of Iran | And 6 more authors.
Scientia Horticulturae | Year: 2012

A set of twelve simple sequence repeat markers was used to evaluate the genetic background of 738 pomegranate (Punica granatum L.) accessions, which belonged to the Iranian National Pomegranate Collection, from 23 provinces of Iran. Genetic diversity among the accessions was analysed using several variability parameters. Forty-three alleles were detected with a mean of 3.59 alleles per locus. The highest levels of polymorphism were found for the locus ABRII-MP26. The mean values of expected heterozygosity and polymorphic information content were 0.521 and 0.458, respectively. Neighbour-Net analysis based on uncorrected P distances and a model-based method separated the accessions into 8 main groups and several subgroups, with a tendency to cluster by geographical origin. From the simple sequence repeat analysis, it can be concluded that the genetic base of the Iranian National Pomegranate Collection is broad enough to ensure future progress in breeding programmes and these markers are effective tools for conducting genetic diversity studies in pomegranate and developing strategies for germplasm management. © 2012 Elsevier B.V. Source

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