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PubMed | Ukrainian Academy of Sciences and National Pirogov Memorial Medical University
Type: | Journal: Problemy radiatsiinoi medytsyny ta radiobiolohii | Year: 2016

The definition of a contribution of the carriage of the G1691A allele of thecoagulation factor V gene and the G20210A allele of the coagulation factor II gene in the development of thrombosis in Ph negative myeloprolifer ative neoplasms (MPN) patients, who were irradiated in the dose range 0,001 0,99 Gy and who were not.The clinical and molecular genetic characteristics of patients with radiation associated and spontaneous polycythemia vera (PV), essential trombotsytemiya (ET) and primary myelofibrosis (PMF) were ana lyzed. The group of radiation associated PV, ET and PMF represented by 35, 10 and 22 patients respectively, and the cohort of spontaneous PV, ET and PMF - 149, 111 and 78 patients respectively.The carriage of any of the two molecular genetic markers of hereditary thrombophilia at spontaneous PMF increases the frequency (3 of 6 vs 8 of 72; p = 0.033) and risk (RR = 6.09; 95 % CI = 1.40-26.43) of thrombosis. The presence of the G1691A allele of the proaccelerin gene in patients with PMF, who were not exposed to ionizing radiation, causes increase the likelihood of venous thrombosis at 10.14 times (95 % CI = 1.67-61.33). At spontaneous and radiation associated Ph negative MPN (in individuals exposed to doses in the range 0,001-0,99 Gy), the higher rate of the occurrence of venous, arterial and any thrombosis was observed in carriers of the G1691A allele the coagulation factor V gene, than in those, whose have the wild type allele. In particular, the G1691A allele of the proaccelerin gene carriers, that are belonged to the group of patients with radiation associated PV, have at 33.33 person years bigger rate of any thrombosis (95 % CI = 0.22-100.00, p = 0.048) and venous vascular events (95 % CI = 12.50-50.00; p = 0.003).In PMF patients with a radiation anamnesis were found the difference (20.00 person years; 95 % CI = 1.51-50.00, p = 0.035) between the ratio of any thrombosis and arterial vascular events, which was calculated for the G1691A allele of the proaccelerin gene and for those, who have the wild type allele. The carriers of the G20210A nucleotide variant of the coagulation factor II gene with spontaneous ET and PMF, compared with patients with the wild type allele, have a higher rate of venous thrombosis per 100 patient years.


Dubok V.,Frantsevich Institute for Problems of Materials Science | Shynkaruk A.,Frantsevich Institute for Problems of Materials Science | Atamanenko O.,Ukrainian Academy of Sciences | Protcenko V.,Ukrainian Academy of Sciences | And 4 more authors.
Materials Science and Engineering B: Solid-State Materials for Advanced Technology | Year: 2010

The objective of the article is to study the synthesis, animal tests and results of clinical applications of the newly proposed kind of bioactive ceramics for full bone tissue restoration-inorganic composites consisting of modified phosphate ceramics, bioactive glasses and bioactive glass-ceramic-so-called SYNTHETBONE materials. Regulation of the composition and structure of the composite can serve to adjust physicochemical and biological properties of the implant with demands of a particular surgery operation and provide the best results of the operations. The presented results demonstrate ample evidence of regulations of resorption rate and mechanism of the composite, favorable biological reaction on its implantation and prove its advantages and high efficiency in numerous surgery operations. © 2009 Elsevier B.V. All rights reserved.


Bothamley G.H.,University of London | Ehlers C.,Center for Research Borstel | Salonka I.,Republican Research and Practical Center for Pulmonology and TB | Skrahina A.,Republican Research and Practical Center for Pulmonology and TB | And 11 more authors.
BMC Pregnancy and Childbirth | Year: 2016

Background: Objectives: To determine whether the incidence of tuberculosis with pregnancy is more common than would be expected from the crude birth rate; to see whether there is significant delay in the diagnosis of tuberculosis during pregnancy. Method: Design: A cross-sectional survey. Setting: 13 tuberculosis clinics within different European countries and the USA. Population/sample: All patients with tuberculosis seen at these clinics for a period > 1 year. Instrument: Questionnaire survey based on continuous data collection. Main outcome measures: number and proportion of women with tuberculosis who were pregnant; timing of diagnosis in relation to pregnancy, including those who were pregnant or delivered in the 3 months prior to the diagnosis of TB and those who developed TB within 3 months after delivery. Results: Pregnancy occurred in 224 (1.5 %) of 15,217 TB patients and followed the expected rate predicted from the crude birth rate for the clinic populations. TB was diagnosed more commonly in the 3 months after delivery (n = 103) than during pregnancy (n = 68; χ 2 = 25.1, P < 0.001). Conclusions: TB is diagnosed more frequently after delivery, despite variations in local TB incidence and healthcare systems. © 2016 The Author(s).


PubMed | National Institute for Tuberculosis, Center for Research Borstel, Agencia de Salut Publica de Barcelona, Hospitalario Universitario Of Pontevedra and 9 more.
Type: Journal Article | Journal: BMC pregnancy and childbirth | Year: 2016

Objectives: To determine whether the incidence of tuberculosis with pregnancy is more common than would be expected from the crude birth rate; to see whether there is significant delay in the diagnosis of tuberculosis during pregnancy.Design: A cross-sectional survey.13 tuberculosis clinics within different European countries and the USA.All patients with tuberculosis seen at these clinics for a period>1year.Questionnaire survey based on continuous data collection.number and proportion of women with tuberculosis who were pregnant; timing of diagnosis in relation to pregnancy, including those who were pregnant or delivered in the 3months prior to the diagnosis of TB and those who developed TB within 3months after delivery.Pregnancy occurred in 224 (1.5%) of 15,217TB patients and followed the expected rate predicted from the crude birth rate for the clinic populations. TB was diagnosed more commonly in the 3months after delivery (n=103) than during pregnancy (n=68; TB is diagnosed more frequently after delivery, despite variations in local TB incidence and healthcare systems.


Kucherenko A.,NASU Institute of Molecular Biology and Genetics | Kucherenko A.,Taras Shevchenko National University | Pampukha V.,NASU Institute of Molecular Biology and Genetics | Bobrova I.,Ukrainian Treatment and Diagnostic Center | And 2 more authors.
Cytology and Genetics | Year: 2015

The aim of this study was to clarify the association between the inosine triphosphate pyrophosphatase (ITPA) gene variants and PEG-IFNα/RBV combination treatment induced anemia in chronic hepatitis C (CHC) Ukrainian patients. The data were collected from 80 CHC patients with HCV genotype 1 infection. All study participants received standard doses of PEG-IFNα and RBV. According to the Hb level changes patients were distributed into: case group—42 patients with combination treatment induced anemia, and control group—38 patients with no signs of anemia. Genotyping for ITPA gene rs1127354 and rs7270101 variants was performed using PCR followed by RFLP assay. Fisher’s exact test was used to estimate the difference in genotype and allelic distribution. Distribution of rs7270101 genotypes was not significantly different between groups of CHC patients with RBV-induced anemia and without it. The frequency of rs1127354 A allele carriers was significantly higher (P < 0.05) in group of CHC patients without anemia (23.7%) comparing to the group of patients with anemia (7.3%). The respective allele frequency in control group (13.2%) was almost 3-fold higher (P < 0.05) comparing to the case group (4.9%). Significant association of ITPA gene rs1127354 with protection against RBV-induced hemolytic anemia was found in Ukrainian patients with CHC infection. Rs1127354 variant may assist as a pharmacogenetic marker in HCV antiviral therapy correction for side effect avoidance. © 2015, Allerton Press, Inc.


Kucherenko A.M.,NASU Institute of Molecular Biology and Genetics | Pampukha V.M.,NASU Institute of Molecular Biology and Genetics | Romanchuk K.Y.,National Pirogov Memorial Medical University | Chernushyn S.Y.,NASU Institute of Molecular Biology and Genetics | And 3 more authors.
Cytology and Genetics | Year: 2016

The aim of this study was to examine association between IFNL4 gene ss469415590 and treatment efficiency in group of Ukrainian PEG-interferon/ribavirin-treated chronic hepatitis C patients. Study group consisted of 92 unrelated hepatitis C virus genotype 1 mono-infected patients: case group–29 patients with late or absent virological response; control group–63 patients with sustained virological response. Study material was genomic DNA. Genotyping was performed using amplification-refractory mutation system PCR. Statistical analysis was performed using GenePop and OpenEpi statistical packages. Obtained results show that ss469415590 ΔG/ΔG genotype is associated with poor virological response (OR = 3.62; CI 95%: 1.12–11.67) in PEG-interferon/ribavirin-treated chronic hepatitis C patients from Ukraine. © 2016, Allerton Press, Inc.


Vernygorodskyi S.V.,National Pirogov Memorial Medical University | Degtiariova L.V.,Bogomolets National Medical University | Iatsyna O.I.,National Cancer Institute | Blume Y.B.,Ukrainian Academy of Sciences | Yemets A.I.,Ukrainian Academy of Sciences
Cytology and Genetics | Year: 2015

The analysis of intestinal differentiation transcription factor CDX2 in the gastric mucosa biopsies has been carried out. It was established that CDX2 by its own promoter activation pathway can obtain intestinal phenotype for gastric mucosa cells. The loss of CDX2 expression in the nuclei of metaplastic epithelium may serve as a predictor of gastric mucosa malignization. © 2015, Allerton Press, Inc.


PubMed | Ukrainian Academy of Sciences and National Pirogov Memorial Medical University
Type: | Journal: Problemy radiatsiinoi medytsyny ta radiobiolohii | Year: 2016

Cytogenetic analysis of the effects of sodium humate in induced irradiation mutagenesis in Allium test.Analysis of the root meristem cells of Allium cepa L. seeds carried by ana telophase. Inves tigated the effect of sodium humate (100 mg/l) on cytogenetic effects irradiation (137Cs) at doses of 5, 10 and 20 Gy.Antimutagenic effect of sodium humate was revealed, what allows to consider it as potential therapeutic modifier of radiation damage. Antimutagenic effect of sodium humate increased with increasing irradiation dose. Spotted differential activity of the drug with respect to various types of aberrations are most effectively reducing the frequency of radiation markers mutagenesis - chromosomal aberrations manifested in lower efficiency of long term survivors against potential changes of chromosomes compared with short lived.Revealed different mechanisms for implementing antimutagenic properties of sodium humate in con dition of irradiation induced mutagenesis in Allium test.


Butov D.O.,Kharkiv National Medical University | Kuzhko M.M.,National Institute Of Phthisiology And Pulmonology Named After F G Yanovskyi Nams Of Ukraine | Makeeva N.I.,Kharkiv National Medical University | Butova T.S.,Kharkiv National Medical University | And 2 more authors.
Pneumonologia i Alergologia Polska | Year: 2016

Introduction: Multi-drug resistant tuberculosis (MDR TB) is a significant health problem in some parts of the world. Three major cytokines involved in TB immunopathogenesis include IL-2, IL-4 and IL-10. The susceptibility to MDR TB may be genetically determined. The aim of the study was to assess the association of IL-2, IL-4, IL-10 gene polymorphisms with multi-drug resistant tuberculosis (MDR TB) in Ukrainian population. Material and methods: We observed 140 patients suffering from infiltrative pulmonary tuberculosis (PT) and 30 apparently healthy subjects. The patients were assigned to two groups whether they suffer or do not suffer from pulmonary MDR TB. Interleukin gene (IL) polymorphisms, particularly T330G polymorphism in the IL-2 gene, C589T polymorphism in the IL-4 gene and G1082A polymorphism in the IL-10 gene were studied through polymerase chain reaction. Circulating levels of IL-2, IL-4 and IL-10 in venous blood were estimated using ELISA. Results: Prior to treatment, patients with PT showed significant increase of IL-2 levels and decrease of IL-4 and IL-10 levels compared to apparently healthy subjects. Circulating IL-4 and IL-10 levels were significantly decreased whilst serum IL-2 level was significantly increased in patients with MDR TB compared to non-MDR TB. Low IL-4 and IL-10 secretion and considerable IL-2 alterations were shown to be significantly associated with mutations of homozygous and heterozygous genotypes affecting C589T polymorphism in the IL-4 gene, G1082A polymorphism in the IL-10 gene and T330G polymorphism in the IL-2 gene in patients with PT. Conclusions: Heterozygous genotype and mutations homozygous genotypes gene in polymorphisms determining specified cytokines’ production is a PT risk factor and may lead to disease progression into chronic phase. Heterozygous genotype of aforementioned cytokine genetic polymorphisms was significantly the most frequent in patients with MDR TB. © 2016 PTChP.


Pampukha V.M.,NASU Institute of Molecular Biology and Genetics | Kravchenko S.A.,NASU Institute of Molecular Biology and Genetics | Moroz L.V.,NASU Institute of Molecular Biology and Genetics | Moroz L.V.,National Pirogov Memorial Medical University | Livshits L.A.,NASU Institute of Molecular Biology and Genetics
Biopolymers and Cell | Year: 2011

Aim. The goal of our study was to develop an accurate detection of the SNP rs12979860 by RFLP-based method and to evaluate the polymorphic genotype distribution for this SNP among individuals with unknown HCV status from Ukraine. Methods.The SNP rs12979860 was tested by PCR RFLP-based method in 99 individuals from Ukraine. Results. The method of accurate detection of the SNP rs12979860 was developed. The genotypes distributions were: CC - 56 %, CT - 34 %, TT - 10 %. Conclusions. Due to the high incidence of CC genotype, found in our study, the SNP rs12979860 analysis may be useful for Ukrainian patients to predict responses to the treatment considering the HCV genotype and viral load. © Institute of Molecular Biology and Genetics, NAS of Ukraine, 2011.

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