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Luo X.-J.,Jiangxi University of Traditional Chinese Medicine | Luo X.-J.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | Liu H.,Jiangxi University of Traditional Chinese Medicine | Liang H.-B.,Nanchang Hangkong University | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2017

Glass transition theory is an important theory in polymer science, which is used to characterize the physical properties. It refers to the transition of amorphous polymer from the glassy state to the rubber state due to heating or the transition from rubber state to glassy state due to cooling. In this paper, the glassy state and glass transition of food and the similar relationship between the composition of Chinese medicine extract powder and food ingredients were described; the determination method for glass transition temperature (Tϵ) of Chinese medicine extract powder was established and its main influencing factors were analyzed. Meanwhile, the problems in drying process, granulation process and Chinese medicine extract powder and solid preparation storage were analyzed and investigated based on Tϵ, and then the control strategy was put forward to provide guidance for the research and production of Chinese medicine solid preparation.


PubMed | Jiangxi University of Science and Technology, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine and Chengdu University of Technology
Type: | Journal: Journal of ethnopharmacology | Year: 2016

Gardenia jasminoides Ellis is a traditional Chinese medicine(TCM) that containing a variety of effective active ingredients and exhibits diverse pharmacological functions, such as anti-inflammatory, antioxidant and nerve protection.This study investigated the effect of Gardenia jasminoides extract (GJE) and Geniposide on learning and memory improvement and neuroprotection in a rat model with chronic cerebral ischemia, as well as explore the underlying mechanisms.The crude GJE was prepared using the methods of water extraction and alcohol precipitation, and refined by macroporous adsorption resin. The chronic cerebral ischemia model was simulated by permanent occlusion of bilateral common carotid arteries in rats. GJE was taken at three doses groups (150mg/kg, 100mg/kg, 50mg/kg), Geniposide group (50mg/kg), and oral administration for 30 days. Memory function was assessed using Morris water maze test. The morphological changes of hippocampus and related parts of brain in rats by Hematoxylin and Eosin (HE) staining were observed. Moreover, the levels of Acetylcholin Esterase (AchE), Nitric Oxide Synthase (NOS), Malondialdehyde (MDA), Superoxide Dismutase (SOD) in the brain tissue were quantified.GJE contained 27% gardenoside and 72% total iridoid glycoside. The chronic cerebral ischemia rat model has been proved successfully. The memory function of the rats assessed using Morris water maze test showed that GJE significantly shortened the escape latency of rats, but had no significant improvement on the number of times crossing the platform and the percentage of time spent in the target quadrant. HE staining showed that the apoptosis and necrosis of the cortex and hippocampus in the GJE group were significantly reduced. In addition, it was found that GJE could significantly improved the content of SOD, inhibited NOS and AchE activity in brain tissue, but did not show a significant reduction in the content of MDA. The effect of medium dosage of GJE was the best among these three dose groups and also better than Geniposide according to the results of all the detection index.GJE had the functions of learning and memory improvement and the neuroprotection on chronic cerebral ischemia model rats. The mechanisms were found to be strongly correlated with antioxygen free radical, reduction of NO toxicity and AChE activity, and brain neuron protective effect. GJE could be able to play a better effect on improving chronic cerebral ischemia than Geniposide.


Su D.,Jiangxi University of Traditional Chinese Medicine | Su D.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | Huang J.,Jiangxi University of Traditional Chinese Medicine | Song Y.,Jiangxi University of Traditional Chinese Medicine | And 3 more authors.
Fitoterapia | Year: 2014

Ainsliaea fragrans Champ, as a well-known herb in Traditional Chinese Medicine, was often used in the treatment of gynecological diseases. Caffeoylquinic acids (CQAs) were the bioactive constituents of this plant medicine which primarily contains mono-CQAs (MCQA) and di-CQAs (DCQA). The biosynthesis showed that MCQAs were the precursor of DCQAs. Recent literatures manifested some particular features of DCQAs, different from MCQAs. Therefore it is apparent that a complete and scientific assessment of DCQAs and MCQAs should include not only the DCQAs' pharmacokinetics and distribution but also its degradation products. So an efficient, sensitive rapid resolution liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of the active ingredients in rat plasma and different tissues had been developed and validated. Mass spectrometric detection was performed by selected reaction monitoring mode (MRM) via an electrospray ionization source operating in negative ionization mode. The method was validated in plasma and tissue samples, which showed good linearity over a wide concentration range (r2 > 0.99), and obtained lower limit of quantification (LLOQ) was 2.34 ng·mL- 1 for the analytes in biological samples. The intra- and inter-day assay variability was less than 15%, and the accuracy was between - 8.8% and 5.7%. This study provided the pharmacokinetic profiles and the tissue regional distribution of MCQAs, DCQAs and caffeic acid. The results indicated that the DCQAs isomers were absorbed quickly after oral administration and degradation products MCQAs were mostly found in tissues, not in plasma. Besides, 1,5-DCQA was the prior configuration for the isomerization phenomenon. The small intestine was the main absorption site for DCQAs. Interestingly, the content of the DCQA and MCQA isomers was all high in the ovary and uterus, and some could pass through the barrier between the blood and brain obviously. © 2014 Elsevier B.V. All rights reserved.


Wei B.,Shenyang Pharmaceutical University | Li Q.,Shenyang Pharmaceutical University | Fan R.,Shenyang Pharmaceutical University | Su D.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | And 6 more authors.
Journal of Mass Spectrometry | Year: 2013

Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, has been clinically used for the treatment of insomnia for centuries. The insomnia mechanism and the possible active ingredients of S. chinensis remain largely unknown. The objective of this study was to develop a method to detect its components which could pass through the blood brain barrier (BBB) by determining the brain microdialysate and brain tissue homogenate samples and then obtain the pharmacokinetic profile in brain for comprehensive understanding of its hypnotic clinical efficacy. Therefore, an efficient, sensitive and selective ultra fast liquid chromatography/tandem mass spectrometry method for the simultaneous determination of six sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyshisandrin, γ-schisandrin and gomisin N) of Schisandra chinensis (Turcz.) Baill. in rat brain tissue homogenate and brain microdialysates has been developed and validated. The analysis was performed on a Shim-pack XR-ODS column (75 mm × 3.0 mm, 2.2 μm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The method was validated in brain homogenate and microdialysate samples, which all showed good linearity over a wide concentration range (r2 > 0.99), and the obtained lower limit of quantification was 0.1 ng · ml-1 for the analytes in brain microdialysate samples. The intra- and inter-day assay variability was less than 15% for all analytes. The study proved the six lignans, as sedative and hypnotic ingredients, could pass through the BBB with brain targeting, distributed mainly in the hypothalamus and possessed complete pharmacokinetics process in brain. The results also indicated that significant difference in pharmacokinetic parameters of the analytes was observed between two groups, while absorptions of these analytes in insomniac group were significantly better than those in normal group. Copyright © 2013 John Wiley & Sons, Ltd. Copyright © 2013 John Wiley & Sons, Ltd.


Nie B.,Jiangxi University of Traditional Chinese Medicine | Du J.,Jiangxi University of Traditional Chinese Medicine | Yu R.,Jiangxi University of Traditional Chinese Medicine | Liu Y.,Jiangxi University of Traditional Chinese Medicine | And 3 more authors.
Communications in Computer and Information Science | Year: 2011

Traditional Chinese Medicine have the secret is dosage, the Traditional Chinese Medical clinical therapeutic effect key is prescription' dosage. The paper aim to explore and analyze the relationship between TCM prescription' dosage and pharmacodynamic effect. The methods: the rats were done into diabetic model,and taken Gegen Qinlian Decoction(Radix Puerariae, Radix Scutellariae, Rhizoma Coptidis, Glycyrrhiza uralensis) which have been design using Uniform Design(UD) method, extract pharmacodynamic effect indexs from the rats, analyze the relationship between TCM prescription' dosage and pharmacodynamic based on Orthogonal Partial Least Square Analysis(OPLS) /O2PLS.The result indicate the order of Variable importance is Radix Puerariae, Rhizoma Coptidis, Glycyrrhiza uralensis, Radix Scutellariae,and the Coefficients of TCM relative to the pharmacodynamic effect indexs. © 2011 Springer-Verlag.


Rao X.-Y.,Beijing University of Chinese Medicine | Rao X.-Y.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | Gong M.,Jiangxi University of Traditional Chinese Medicine | Yin S.,Jiangxi University of Traditional Chinese Medicine | And 8 more authors.
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To investigate the intestinal absorption characteristics of Pulsatilla saponin D in rats. Methods: In situ single-pass intestinal perfusion model was used to inspect the absorption of Pulsatilla saponin D in the intestinal tract of rats. HPLC was used to determine the concentration of Pulsatilla saponin D in intestinal perfusion fluid samples. Absorption rate constant (Ka) and apparent permeability coefficient (Papp) were used as indexes to investigate the effects of absorption sites, drug concentration, different pH values, and P-glycoprotein (P-gp) inhibitor on Pulsatilla saponin D absorption. Results: There was the significant difference (P<0.05) of intestinal perfusion fluid samples in each intestinal segment and the order of the Ka and Papp values of Pulsatilla saponin D in each intestinal segment was colon>ileum>jejunum>duodenum. With the pH value increasing, the Ka and Papp values also increased and both of them had significant differences (P<0.05). The colon absorption of perfusion fluid at different concentration (0.30, 0.15, and 0.08 mg/mL) had no significant difference (P>0.05); There was significant difference (P<0.05) in Ka and Papp values with and without P-gp inhibitor. Conclusion: Pulsatilla saponin D could be well absorbed in whole intestinal segments of rats, and the best intestinal absorption site is colon; The drug concentration in a certain range has no effect on Ka and Papp values, which preliminarily comfirms that the obsorption mechanism of Pulsatilla saponin D could be passive diffusion; Pulsatilla saponin D may be a substrate of P-gp and possess the saturation phenomenon of transporters.


Wang Y.-W.,Jiangxi University of Traditional Chinese Medicine | Li Z.-F.,Jiangxi University of Traditional Chinese Medicine | Li Z.-F.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | He M.-Z.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | And 6 more authors.
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To study the chemical constituents of Gastrodia elata. Methods: The constituents were isolated by various chromatographies, including preparative TLC, silica gel, Sephadex LH-20, and ODS columns, and their structures were elucidated on the basis of their physicochemical properties and spectral data. Results: Eleven compounds were isolated from 70% EtOH fraction eluted from resin column and their structures were identified as gastrodin (1), 4-hydroxybenzyl alcohol (2), 4-hydroxybenzoic acid (3), 4-hydroxybenzaldehyde (4), 4-hydroxybenz-yl methyl ether (5), parishin (6), 4-(methylsulfinylmethyl) phenol (7), dibutyl phthalate (8), dioctyl phthalate (9), 4, 4'-dihydroxydiphenyl methane (10), and grossamide (11). Conclusion: Compound 7 is a new natural product, and compounds 9 and 11 are isolated from the plants of Orchidacee for the first time.


Zhou C.,Shenyang Pharmaceutical University | Zhou C.,Liaoning Provincial Institute for Control of Food and Drugs | Liu Y.,Liaoning Provincial Institute for Control of Food and Drugs | Su D.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | And 7 more authors.
Chromatographia | Year: 2011

We report the development and validation of a rapid, specific, and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method for analysis and pharmacokinetic study, in rats, of hyperoside and isoquercitrin, two bioactive structural isomers present in the leaves of Apocynum venetum L. After simple deproteinization by addition of acetonitrile, the analytes were separated on a C18 column. Detection was by tandem mass spectrometry in multiple reaction monitoring mode. The method was linear over the concentration range 3.9-195 ng mL-1 for both hyperoside and isoquercitrin. Intra-day and inter-day precision for both hyperoside and isoquercitrin were <13.1%, and relative errors were all within 7.1% at all QC levels. The method was used to study the pharmacokinetic performance of the compounds after oral administration of an extract of Apocynum venetum L. leaves to rats. © 2011 Springer-Verlag.


PubMed | Jiangxi University of Traditional Chinese Medicine and National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2016

In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in Flos Scabiosae (FS). First, Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was developed for the identification of the multi-constituents in FS. A total of 48 compounds (9 phenolic acids, 24 flavonoids, 8 iridoids and 7 others) were unambiguously or tentatively identified, including 25 compounds (flavonoids, phenolic acids) identified in FS for the first time. Second, ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometry (UHPLC-MS-MS) was developed for the quantitative analysis of 10 phenolic compounds. Ten compounds, either with high contents or strong bioactivities, were chosen as markers. This analytical method was validated through intra- and inter-day precision, repeatability and stability, with respective relative standard deviations <4.43, <8.64, <4.60 and <3.65%, respectively. The limits of detection and quantification were <1.09 and <16.96 ng/mL, respectively. The overall recoveries ranged from 96.47 to 103.94% .Then the validated method was applied to determine 10 batches of FS. The results indicated that the new method can be applied for the qualitative and quantitative analysis of FS.


Gu B.,Jiangxi Science and Technology Normal University | You J.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine | Li Y.,Jiangxi Science and Technology Normal University | Duan C.,Chinese Institute of Materia Medica | Fang M.,National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine
European Food Research and Technology | Year: 2010

Garlic (Allium sativum L.) has been used as a medicinal agent for thousands of years. Its various pharmacological properties in vivo and in vitro have been widely verified, specifically effective against bacterial, viral, fungal and parasitic infection. Currently, garlic oils extracted by steam distillation technique were complexed with 2-hydroxypropyl-beta-cyclodextrin using high-energy ball milling method, and then inclusion complex was incorporated with enteric-coated powder to reduce its gastrointestinal irritant. In accordance with the maximum tolerance dose test, mice given enteric-coated garlic tablet by gavage at 3 g/kg BW dosage observed no symptoms of acute toxicity. In this study, experiment animals were randomly assigned to orally expose to different doses of garlic tablet for 30 consecutive days, and eventually their immune functions were entirely analyzed. Dietary supplementation with garlic tablet did not affect the growth of normal mice and relative weight to immune organs (thymus and spleen) during the experiment. As compared with the control, garlic tablet intensively enhanced ConA-induced splenetic lymphocyte proliferation, serum hemolysin production, the number of hemolytic plague forming cells, phagocytic activity of peritoneal macrophages, and carbon clearance rate at the dose of 400, 600 mg/kg BW. Furthermore, it also significantly facilitated DNFB-induced delayed-type hypersensitivity reaction and NK cells cytoxicity at the dose of 600 mg/kg BW. Additionally, garlic tablet markedly augmented the secretion of serum cytokines (TNF-α, IL-2) in a dose-dependent manner. The present findings suggest that dietary supplementation with garlic tablet could potentiate mononuclear phagocytic system function, cell-mediated immunity, and humoral immunity of normal mice in varying degrees. Taken together, enhancement of immune functions in vivo implied that enteric-coated garlic tablet deserved to be developed as an economic and potential functional food. © Springer-Verlag 2009.

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