Development of a UFLC-MS/MS method for simultaneous determination of six lignans of Schisandra chinensis (Turcz.) Baill. in rat plasma and its application to a comparative pharmacokinetic study in normal and insomnic rats
Wei B.,Shenyang Pharmaceutical University |
Li Q.,Shenyang Pharmaceutical University |
Su D.,The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine |
Su D.,Jiangxi University of Traditional Chinese Medicine |
And 7 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013
Schisandra chinensis (Turcz.) Baill., a traditional Chinese medicine, has been used for treating insomnia for centuries. This paper was designed to study on the plasma pharmacokinetic for its absorption process, and to compare the pharmacokinetics of its active ingredients in normal and insomnic rats orally administrated with the prescription. Therefore, an efficient, sensitive and selective ultra fast liquid chromatography/tandem mass spectrometry (UFLC-MS/MS) method for the simultaneous determination of six sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyshisandrin, γ-schisandrin and gomisin N) of Schisandra chinensis (Turcz.) Baill. in rat plasma has been developed and validated. The analysis was performed on a Shim-pack XR-ODS column (75mm×3.0mm, 2.2μm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid waterat a flow rate of 0.4ml/min. The detection of the analytes was performed on 4000Q UFLC-MS/MS system with turbo ion spray source in the positive ion and multiple reaction-monitoring mode. The method was validated in plasma samples, which showed good linearity over a wide concentration range (r2>0.99), and obtained lower limits of quantification were 10, 1.2, 1.2, 1.2, 1.0 and 1.2ngmL-1 for the analytes. The intra- and inter-day assay variability was less than 15% for all analytes. The mean extraction recoveries of analytes and IS from rats plasma were all more than 85.0%. The validated method has been successfully applied to comparing pharmacokinetic profiles of analytes in rat plasma. The results indicated that significant difference in pharmacokinetic parameters of the analytes was observed between two groups, while absorptions of these analytes in insomnic group were all significantly higher than those in normal group. © 2013.
Yin W.-J.,Jiangxi University of Traditional Chinese Medicine |
Wei H.-Z.,Jiangxi University of Traditional Chinese Medicine |
Liu S.-N.,Jiangxi University of Traditional Chinese Medicine |
Jin H.-X.,The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine |
And 5 more authors.
Chinese Traditional and Herbal Drugs | Year: 2016
Objective: To develop a method for controlling the quality consistency of Qianlie Shule Capsule (QSC) by blending raw marerials. Methods: Blending raw materials were blended by medicine calculator V2. The content differences of epimedin C and icariine in QSC made from raw materials and their mixtures were compared. Results: The RSD of index component epimedin C from blended and raw materials were 11.2% and 82.5%, the RSD of icariine were 8.70% and 35.5%, the difference between index components decreased obviously, and it showed that the quality consistency in QSC was greatly increased. Conclusion: Using the method to control the quality coherence of Chinese patent medicine has the obvious results, it is easy to operate and suitable for actual production. © 2016, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.
Wei H.-Z.,Jiangxi University of Traditional Chinese Medicine |
Guo Q.,Jiangxi University of Traditional Chinese Medicine |
Feng Y.-L.,Jiangxi University of Traditional Chinese Medicine |
Zhang W.-G.,Jiangxi University of Traditional Chinese Medicine |
And 3 more authors.
Chinese Traditional and Herbal Drugs | Year: 2014
Objective: To research the separation, extraction, and quality control of tiliroside from Loropetalum chinense. Methods: The herbs were extracted using ethanol reflux extraction, and then through macroporous resin, depressure silica gel column chromatography, and elution, tiliroside monomer was obtained. Content was determined by the C18 column, mixture of methanol-0.1% phosphoric acid (55:45) as mobile phase at 1.0 mL/min, and UV detection wavelength at 320 nm were used for the determination. Results: Tiliroside can be a good separation of the monomer, with the content of 98%; Tiliroside was better in linear relationship in the range of 0.041-0.513 μg (r = 0.9997). The recovery rate was 100.05%, RSD = 1.50%. Conclusion: Using this method, tiliroside can be better extracted and separated, Meanwhile the determination method is very stable and accurate, and has good repeatability, which can be used for the quality control of tiliroside from L. chinense.
Qualitative and quantitative analysis of amygdalin and its metabolite prunasin in plasma by ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry
Gao G.,Jiangxi University of Traditional Chinese Medicine |
Wang Y.,Chinese Institute of Materia Medica |
Wei H.,Jiangxi University of Traditional Chinese Medicine |
Ouyang H.,The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine |
And 5 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2014
A method was developed for the determination of amygdalin and its metabolite prunasin in rat plasma after intragastric administration of Maxing shigan decoction. The analytes were identified by ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and quantitatively determined by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry. After purified by liquid-liquid extraction, the qualitative analysis of amygdalin and prunasin in the plasma sample was performed on a Shim-pack XR-ODS III HPLC column (75 mm × 2. 0 mm, 1.6 μm), using acetonitrile-0.1% (v/v) formic acid aqueous solution. The detection was performed on a Triple TOF 5600 quadru-pole time of flight mass spectrometer. The quantitative analysis of amygdalin and prunasin in the plasma sample was performed by separation on an Agilent C18 HPLC column (50 mm×2. 1 mm, 1. 7 μm), using acetonitrile-0. 1% (v/v) formic acid aqueous solution. The detection was performed on an AB Q-TRAP 4500 triple quadrupole mass spectrometer utilizing electrospray ionization (ESI) interface operated in negative ion mode and multiple-reaction monitoring (MRM) mode. The qualitative analysis results showed that amygdalin and its metabolite pruna-sin were detected in the plasma sample. The quantitative analysis results showed that the linear range of amygdalin was 1. 05-4 200 ng/mL with the correlation coefficient of 0. 999 0 and the linear range of prunasin was 1. 25-2 490 ng/mL with the correlation coefficient of 0. 997 0. The method had a good precision with the relative standard deviations (RSDs) lower than 9. 20% and the overall recoveries varied from 82. 33% to 95. 25%. The limits of detection (LODs) of amygdalin and prunasin were 0. 50 ng/mL. With good reproducibility, the method is simple, fast and effective for the qualitative and quantitative analysis of the amygdalin and prunasin in plasma sample of rats which were administered by Maxing shigan decoction.
Wei H.-Z.,The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine |
Zhang W.-P.,Jiangxi University of Traditional Chinese Medicine |
Mao H.-M.,The Third Peoples Hospital of Jiujiang |
Rao Y.,The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine |
And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2013
Objective: To establish a fast detection method during the purifying process of the extracts from Paeoniae Alba Radix based on near infrared optical fiber transmission spectroscopy. Methods: The near infrared spectroscopy (NIRS) of the eluates by water and 70% ethanol which flowed through the macroporous resin D-101 was measured by NIR transmission optical fiber probe, and the method of pattern recognition was used to study the two processes. Aiming at the process of 70% ethanol elution, the contents of constituents were determined by HPLC as the reference values, and partial least square regression (PLSR) was applied to build the calibration models of paeoniflorm and albiflorin. Results: The processes of water elution and 70% ethanol elution could be accurately monitored with pattern recognition, and the changes of the solvent system could be accurately indicated. The quantitative models were applied to successfully predict the elution curves of Paeoniae Alba Radix extracts from the same batch and different batches on macroporous resin. The root mean square errors of prediction (RMSEP) calculated by PLSR models for paeoniflorm and albiflorin were 0.124, 0.172, and 0.120, 0.133, respectively, and the correlation coefficient between the values calculated by NIRS and HPLC was all greater than 0.992. Conclusion: The method is real time, efficient, and fast, and could be applied to the fast analysis for the purifying process of Chinese materia medica.