National Pedagogical University of Ukraine

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Kiev, Ukraine
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Falfushxnska H.I.,National Pedagogical University of Ukraine | Romanchuk L.D.,National Pedagogical University of Ukraine | Stoliar O.B.,National Pedagogical University of Ukraine
Ukrain'skyi Biokhimichnyi Zhurnal | Year: 2010

The metal-buffering and stress proteins metallothioneins (MTs) of frog are characterised by unusually high content of copper as for vertebrate animals and instability that was shown in our previous studies. They easily lost copper and especially zinc under unfavourable conditions. The aim of this study was to examine the reactivity of SH groups in the MTs from the liver of frog Rana ridibunda after the effect of Cu2+ (0.01 mg/1) and Zn2+ (0.1 mg/l) ions on the organism during 14 days. The a-and β-domains of MTs with molecular weights of about 4 kDa were separated by the size-exclusion chromatography on Sephadex G-50. Unlike higher vertebrates, frogs demonstrated higher reactivity of α-domain than β-domain with the Ellman's reagent (DTNB). The signs of partial oxidations in β-domain included the creation of by-products with molecular weight about 12 kDa, low reactivity of SH-groups, and typical of-S-S-bonds peculiarities of UV-spectra. The effect of both metal ions on frog provoked the elevation of SH-groups reactivity in α-domain with the appearance of by-product with molecular weight of 16 kDa and its reduction in β-domain. The incubation of MTs of control animals with 0.5 and 5.0 mM of H2O2 did not affect its chromatographic characteristics. In the frogs loaded by Cu2+ and Zn2+ the effect of 5.0 mM H2O2 on MTs provoked the release of 4 kDa product. So the α-domain is responsible for the increased release of metals from injured MTs in frogs, whereas extremely high oxidizability of β-domain makes its participation in the exchange of metals elusive and provokes the aggregation of MTs.


Falfushynska H.I.,National Pedagogical University of Ukraine | Gnatyshyna L.L.,National Pedagogical University of Ukraine | Stoliar O.B.,National Pedagogical University of Ukraine
Ukrain'skyi Biokhimichnyi Zhurnal | Year: 2012

Tetrazine pesticides are widely used for the treatment of crops in most EU countries and USA. However, data about the effect of environmentally realistic concentrations of biocides on the molecular stress response system in non-target organisms are absent. The aim of our study was the comparison of adaptive capability of bivalve mollusk Anodonta cygnea from two populations under the effects of commercial pesticide Apollo in terms of biochemical parameters of the digestive gland. The differences between parameters of oxidative stress and glutathione transferase activity in specimens of control groups from clean (I group) and polluted (B group) areas have been shown. Under the effect of Apollo, the level of protein carbonyls and microsomal oxidation processes increased, and the level of metallothioneins and oxyradical formation decreased in the specimens from both populations. However, the treatment provoked the activation of antioxidant processes in the I group and their inhibition in B group. Potentially the injury of cellular thiols, glutathione and metallothioneins, seems to be key point of tetrazine pesticides toxicity.


Stolbov D.V.,Hebei Normal University | Olefirenko N.V.,National Pedagogical University of Ukraine
Computer, Intelligent Computing and Education Technology - Selected Peer Reviewed Papers From 2014 International Conference on Computer, Intelligent Computing and Education Technology, CICET 2014 | Year: 2014

In this paper, we focus our attention on the approach of development educational software for teaching students the Internet safety by example of the Social Network Simulator (SNS simulator). We present the features of teaching students security in social network sites based on their activities in the Internet. Then, on the basics of these features we build requirements for the SNS simulator. According to these requirements, we define the design model and select programming tools for the SNS simulator implementation in practice. To conclude, we describe an example of working in the SNS simulator. © 2014 Taylor & Francis Group.


Buketov A.,General Maritime | Maruschak P.,Kharkiv Polytechnic Institute | Sapronov O.,General Maritime | Zinchenko D.,General Maritime | And 2 more authors.
Transport | Year: 2016

Coatings based on epoxy diane oligomer ED-20 are being developed as environmentally benign, foulingresistant marine coatings. Silver oxide and carbonate (mixture) was used as filler, the dispersion of which is 0.5 µm. It is proved that for the formation of composites with the improved physical and mechanical properties it is necessary to add Mechanical Mixture of Silver Carbonate and Oxide (MMSCO) in the amount of q = 0.050 … 0.250 pts. wt. into the epoxy diane oligomer ED-20 (100 pts. wt.). The obtained values of impact toughness of composites during the introduction of MMSCO into the binder increased by 2.5 times compared to the epoxy matrix. Moreover, the maximum capacity to resist impact loads, including fracture toughness, was demonstrated by the Composite Material (CM) with the filler content q = 0.050 pts. wt. Impact toughness of this CM is а = 18.53 kJ/m2. The developed materials and protective coatings based on them were used on the dry cargo ship Oles Honchar. © 2016 Vilnius Gediminas Technical University (VGTU) Press.


PubMed | National Pedagogical University of Ukraine
Type: Journal Article | Journal: Ukrains'kyi biokhimichnyi zhurnal (1999 ) | Year: 2011

The metal-buffering and stress proteins metallothioneins (MTs) of frog are characterised by unusually high content of copper as for vertebrate animals and instability that was shown in our previous studies. They easily lost copper and especially zinc under unfavourable conditions. The aim of this study was to examine the reactivity of SH groups in the MTs from the liver of frog Rana ridibunda after the effect of Cu2+ (0.01 mg/l) and Zn2+ (0.1 mg/l) ions on the organism during 14 days. The alpha- and beta-domains of MTs with molecular weights of about 4 kDa were separated by the size-exclusion chromatography on Sephadex G-50. Unlike higher vertebrates, frogs demonstrated higher reactivity of alpha-domain than beta-domain with the Ellmans reagent (DTNB). The signs of partial oxidations in beta-domain included the creation of by-products with molecular weight about 12 kDa, low reactivity of SH-groups, and typical of -S-S-bonds peculiarities of UV-spectra. The effect of both metal ions on frog provoked the elevation of SH-groups reactivity in a-domain with the appearance of by-product with molecular weight of 16 kDa and its reduction in beta-domain. The incubation of MTs of control animals with 0.5 and 5.0 mM of H2O2 did not affect its chromatographic characteristics. In the frogs loaded by Cu2+ and Zn2+ the effect of 5.0 mM H2O2 on MTs provoked the release of 4 kDa product. So the alpha-domain is responsible for the increased release of metals from injured MTs in frogs, whereas extremely high oxidizability of beta-domain makes its participation in the exchange of metals elusive and provokes the aggregation of MTs.


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