National Institute for Oncology

Budapest, Hungary

National Institute for Oncology

Budapest, Hungary
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Chaouki W.,National Institute for Oncology | Meddah B.,Laboratory of Pharmacology and Toxicology | Hmamouchi M.,Mohammed V University
Pharmazie | Year: 2015

Daphne gnidium L. (Thymeleacees) is a famous Moroccan plant with cancer-related ethnobotanical use. Previously, we demonstrated that ethyl acetate extract of D. gnidium had antiproliferative and pro-apoptotic potential on human breast tumor MCF-7 cells. The purpose of this study was to investigate if the antiproliferative effect of this extract was similar for different human cancer cell lines such as A549 lung cancer and SMMC-7721 hepatoma cells. Moreover, this work essentially focused on the intrinsic apoptotic signaling pathway. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide on A549 and SMMC-7721 cells. The characterization of the mechanisms involved in this effect was determined by lactate dehydrogenase test, apoptosis assays and western blot analyses. Our present study has shown that this extract strongly inhibited proliferation of A549 (IC50: 213±15μg/ml) and SMMC-7721 (IC50: 170±13μg/ml) cells. The characterization of antiproliferative effect demonstrated that this extract was an apoptosis inducer in both cell lines tested. The results of western blot analyses have shown in SMMC-7721 cells that this extract activated caspase signaling triggered by the modulation of Bcl-2 family proteins. These findings suggest that this natural extract-induced effects may have novel therapeutic applications for the treatment of different cancer types.


Muller J.,Semmelweis University | Illes A.,Debrecen University | Molnar Z.,National Institute for Oncology | Rosta A.,National Institute for Oncology | And 2 more authors.
Journal of Pediatric Hematology/Oncology | Year: 2011

Background: The aim of our work was to compare the treatment modalities and the survival rates in adolescents (14 to 21 y) with Hodgkin lymphoma (HL) treated with adult (A) or with pediatric (P) regimens. Procedure: From January 1990 to December 2004, 134 (A) and 111 (P) adolescents with HL were treated. Male:female ratio was 1:1.48 (A) and 1:1.36 (P), the mean-age 18.6 (A) and 15.8 years (P), respectively. Results: The patients were treated either with doxorubicin, bleomycin, vinblastine and dacarbazine (A) or with OPPA/OEPA±COPP regimens (P). About 82% (A) and 89% (P) of the patients received radiotherapy. Relapse rates were 13% (A) and 14% (P). Fourteen patients died in group (A) and 9 in group (P). There were no significant differences in the overall survival and event-free survival rates at 5 and 10 years between the 2 patient's groups. For children under age of 18 years old overall survival was 92.8±3% at 5 and 89.6±3% at 10 years in group (P) and 89.4±4% at 5 years and 83.1±6% at 10 years (P=0.2822) in group (A). For children under the age of 18 years event-free survival was 82.4±4% at 5 and 10 years in group (P) and 69.6±7% at 5 years and 59.1±8% at 10 years (P=0.0192) in group (A). Conclusion: In case of the patients younger than 18 years, the survival rates are more favorable by using pediatric regimens, so these patients might have a benefit if they are treated in pediatric institutes. Copyright © 2011 by Lippincott Williams & Wilkins.


Chaouki W.,National Institute for Oncology | Leger D.Y.,Laboratory of Biochemistry | Eljastimi J.,Laboratory of Biochemistry | Beneytout J.-L.,Laboratory of Biochemistry | Hmamouchi M.,National Institute of Medicinal and Aromatic Plants
Pharmaceutical Biology | Year: 2010

Aristolochia baetica L. (Aristolochiaceae) and Origanum compactum Benth. (Lamiaceae) are native plants of Morocco used in traditional medicine. In order to systematically evaluate their potential activity on human breast cancer, four different polarity extracts from each plant were assessed in vitro for their antiproliferative effect on MCF-7 cells. As a result, several extracts of those plants showed potent cell proliferation inhibition on MCF-7 cells. Chloroform extract of A. baetica (IC50: 216.06±15 μg/mL) and ethyl acetate of O. compactum (IC50: 279.51±16 μg/mL) were the most active. Thin layer chromatography examination of the bioactive extracts of A. baetica and O. compactum showed the presence of aristolochic acid and betulinic acid, respectively. These results call for further studies of these extracts. © 2010 Informa UK Ltd.


PubMed | National Institute for Oncology
Type: Comparative Study | Journal: Pharmaceutical biology | Year: 2010

Aristolochia baetica L. (Aristolochiaceae) and Origanum compactum Benth. (Lamiaceae) are native plants of Morocco used in traditional medicine. In order to systematically evaluate their potential activity on human breast cancer, four different polarity extracts from each plant were assessed in vitro for their antiproliferative effect on MCF-7 cells. As a result, several extracts of those plants showed potent cell proliferation inhibition on MCF-7 cells. Chloroform extract of A. baetica (IC50: 216.06 +/- 15 microg/mL) and ethyl acetate of O. compactum (IC50: 279.51 +/- 16 microg/mL) were the most active. Thin layer chromatography examination of the bioactive extracts of A. baetica and O. compactum showed the presence of aristolochic acid and betulinic acid, respectively. These results call for further studies of these extracts.

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