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Hyderabad, India

Sinha S.N.,Indian Institute of Toxicology Research | Vishnu Vardhana Rao M.,National Institute Nutrition | Vasudev K.,Indian Institute of Toxicology Research | Odetokun M.,Pesticide Laboratory
Food Control | Year: 2012

The LC-MS/MS with Quick, Easy, Cheap, Effective, Rugged and safe method was used for analysis of eighteen pesticides in fruit samples. This method was found to be accurate (≥99%), as it possessed limits of detection in the 0.002-0.087 ranges respectively. The coefficients of variations (≥0.9999) were less than 2% at the low ngg -1 concentration. Mean recoveries ranged between 97 and 101%, and % RSD were below 5%. The imidacloprid mean concentrations of red grapes (125.124ngg -1) and green grapes (702.030ngg -1) differed significantly (p<0.05) between the grapes. Similarly, the fenitrothion mean concentration of red grapes (143.66ngg -1) and green grapes (51.554ngg -1) differed significantly (p<0.001) between the fruits. The average concentration of quinalphos was 4.317 and 1.389ngg -1 differed significantly (p<0.01) between the grapes. In apples imidacloprid, quinalphos, triazophos, ethion and acephate were also present. This study may be helpful in developing a regional exposure database and in the facilitation of health risk assessment due to pesticide exposure. © 2011 Elsevier Ltd. Source


Sinha S.N.,National Institute of Nutrition Hyderabad Indian Council of Medical Research | Rao M.V.V.,National Institute Nutrition | Vasudev K.,National Institute of Nutrition Hyderabad Indian Council of Medical Research
Food Research International | Year: 2012

In this study, we assessed the exposure of urban populations to different classes of organophosphate pesticides due to the consumption of different types of vegetables. Liquid chromatography-mass spectrometry was used for quantification, while the quick, easy, cheap, effective, rugged and safe extraction method was used to isolate eighteen organophosphate pesticides found in vegetable samples (eggplant, ladyfinger, cauliflower, cabbage, tomato and chili) at concentration of μg/kg. This method was accurate (≥99.5%) and possessed a limit of detection and quantification in the range of 0.002-0.099 and 0.009-0.337μg/kg respectively. The coefficients of variation (≥0.9999) were less than 2% at the low end of the linear range of the method. The mean recovery ranged between 94 and 103%, and the % relative standard deviation (RSD) was generally below 10%. These results demonstrate that the methodology is both highly efficient and robust. The proposed method was successfully applied to the analysis of vegetable samples collected from different government farmer markets and street shops in urban areas. The mean concentration of chlorpyrifos in eggplant (24.02μg/kg), cabbage (10.55μg/kg), cauliflower (2.85μg/kg), tomato (178.87μg/kg) and ladyfinger (2.49μg/kg) differed significantly (p < 0.002). Similarly, the mean concentration of triazophos in eggplant (0.863μg/kg), cabbage (2.21μg/kg), cauliflower (0.491μg/kg), tomato (3.01μg/kg) and ladyfinger (2.49μg/kg) differed significantly (p < 0.007). A similar trend was observed for acephate, fenitrothion and phosalone. This study may be helpful in developing a regional exposure database and in facilitating assessment of health risks from pesticide exposure in our day-to-day lives. © 2011 Elsevier Ltd. Source


Basak S.,University of Oslo | Basak S.,National Institute Nutrition | Das M.K.,University of Oslo | Srinivas V.,National Institute Nutrition | Duttaroy A.K.,University of Oslo
Molecular and Cellular Biochemistry | Year: 2014

The study aims to investigate the impacts of hyperglycemia in the presence of fatty acids on early placentation processes that involves tube formation, cellular growth and proliferation, and metabolic activities of the first trimester trophoblast cells. Effects of maternal circulatory glucose levels that mimic physiological (5.5 mM), pre-diabetic (11 mM) and diabetic (≥25 mM) phenotypes on tube formation (as a measure of angiogenesis in vitro), cellular viability and proliferation, fatty acid uptake and expression of genes associated with invasion, angiogenesis and fatty acid metabolism were examined using HTR8/SVneo cells. Glucose (25 mM) induced tube formation, viability, and proliferation of the first trimester trophoblast cells, HTR8/SVneo. Tube formation was, however, disintegrated in the presence of high glucose (40 mM) which was partially protected by eicosapentaenoic acid, 20:5n-3 (EPA) and docosahexaenoic acid, 22:6n-3 in vitro. Glucose (25 mM)-mediated induction in tube formation was favored by increased cellular uptake of [14C]EPA (p < 0.05). Treatment of HTR8/SVneo cells with glucose (25 mM) significantly increased mRNA and protein level of matrix metalloproteinase-9 (MMP9) (p < 0.05). In addition, glucose (25 mM) stimulated the expression of fatty acid binding protein-4, FABP4, and plasma membrane fatty acid binding protein, FABPpm, in these cells (p < 0.05). Glucose-stimulated tube formation in a ‘concentration-dependent’ manner, and this may involve activation of several factors that include MMP9 and fatty acid uptake and metabolism. © 2014 Springer Science+Business Media New York Source

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