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Jakarta, Indonesia

to develop an assay system of a radioisotope (32P)-based PCR dot-blot hybridization technique and evaluation of the assay directly for TB sputum samples to detect mutation at codon 306 of embB gene of Mycobacterium tuberculosis related with ethambutol (EMB) resistance. one hundred and sixteen of sputum samples were used in this study. Bacterial genome in sputum samples was extracted and tested for mutation at codon 306 of embB gene by the developed PCR dot blot assay using a radioisotope (32P)-labeled oligonucleotide. The positive results were confirmed by DNA sequencing. all 116 sputum samples were PCR positive for M. tuberculosis. Of 116 samples, three (2.59%) were EMB resistant-M. tuberculosis (MTB) and showed a substitution mutation (ATG/Met'-->GTG/Val) at codon 306 of embB gene. None of mutation was detected at codon 299 of embB gene. we successfully developed a radioisotope (32P)-based PCR dot blot hybridization technique for detection of mutation at codon 306 of embB gene related with EMB resistant M. tuberculosis. The assay can detect a large number of samples that is suitable for monitoring, surveillance, and epidemiology studies. Source

Darsono,National Nuclear Energy Agency
Macromolecular Symposia | Year: 2015

Summary An experiment on surface coating of sengon wood (Paraserianthes falcataria L. Nielsen) using ultra violet (UV) irradiation has been conducted. Epoxy acrylate and urethane acrylate resins were used as coating materials after addition of tripropylene glycol diacrylate and a photoinisiator, Darocure 1173. Irradiation was conducted using UV light at the conveyor speed of 2, 3, 4, and 5 m/min. Parameters observed were glossyness, adhesion, hardness, abrasion resistance and chemical, solvent and stain resistance. The superior results of the epoxy acrylate films were obtained to show excellent adhesion on the surface of wood meeting with the standard because of greater remaining values than 50% (ASTM 2571-71), together with the high degrees of abrasion, hardness, glossyness and torelance against chemicals, solvent, and stain except against 10% NaOH solution. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Tjandrawinata R.R.,Dexa Laboratories of Biomolecular science | Trisina J.,Dexa Laboratories of Biomolecular science | Rahayu P.,Dexa Laboratories of Biomolecular science | Prasetya L.A.,Dexa Laboratories of Biomolecular science | And 2 more authors.
Drug Design, Development and Therapy | Year: 2014

DLBS1033 is a bioactive protein fraction isolated from Lumbricus rubellus that tends to be unstable when exposed to the gastrointestinal environment. Accordingly, appropriate pharmaceutical development is needed to maximize absorption of the protein fraction in the gastrointestinal tract. In vitro, ex vivo, and in vivo stability assays were performed to study the stability of the bioactive protein fraction in gastric conditions. The bioactive protein fraction DLBS1033 was found to be unstable at low pH and in gastric fluid. The “enteric coating” formulation showed no leakage in gastric fluid–like medium and possessed a good release profile in simulated intestinal medium. DLBS1033 was absorbed through the small intestine in an intact protein form, confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) analysis. This result confirmed that an enteric coating formula using methacrylic acid copolymer could protect DLBS1033 from the acidic condition of the stomach by preventing the release of DLBS1033 in the stomach, while promoting its release when reaching the intestine. From the blood concentration–versus-time curve, 99mTc-DLBS1033 showed a circulation half-life of 70 minutes. This relatively long biological half-life supports its function as a thrombolytic protein. Thus, an enteric delivery system is considered the best approach for DLBS1033 as an oral thrombolytic agent. © 2014 Tjandrawinata et al. Source

Suwarno H.,National Nuclear Energy Agency
Advanced Materials Research | Year: 2011

The synthesis and characterizations of Fe-Ti and Mg-Ti-Fe alloys with the atomic ratio of Fe:Ti = 2:1 and Mg:Ti:Fe = 2:5:6 prepared by mechanical alloying technique in toluene solution and measurement the hydrogen absorption properties of the yields have been performed. The Fe-Ti and Mg-Ti-Fe elemental powders are milled with the milling time of 30 h, in a Mixer Mill, type PW 700i high energy ball mill. The milled specimens are analyzed with an X-ray diffractometer, Philip, type PW 1710, using Cu as the anode tube and λ = 1.5406 Å. Qualitative and quantitative analyses are determined using Rietveld method developed by Fuji Izumi. The microstructure of the specimens after milling and hydriding are identified with a scanning electron microscope, Philip type 550. The refinement analysis of the x-ray diffractions results for Fe-Ti alloy shows that before milling the specimen consists of Ti and Fe phases, and after 30 h of milling new phases identified as FeTi and Fe2Ti are formed. In case of Mg-Ti-Fe alloy after 30 h of milling new phases identified as Fe2Ti and FeTi compounds are formed with the absence of Mg-Ti and Mg-Fe. Quantitative analysis of the milled powders shows that the mass fractions of FeTi and Fe2Ti phases are correspondingly 22.5 wt% and is 21.1 wt%, and the rest is Fe. The disappearance of Mg peaks after milling is suggested from the transformation of the crystallite into amorphous state. On hydriding at room temperature, the milled Fe-Ti and Mg-Fe-Ti powders are transformed into β-Ti4FeH8.5, Fe and TiH2. Considering the high hydrogen capacity and the low hydriding temperature of the Mg-Ti-Fe alloy compared to those of Fe-Ti alloy, the Mg-Ti-Fe alloy could be promoted as a new hydrogen storage material. © (2011) Trans Tech Publications, Switzerland. Source

Sudjadi U.,National Nuclear Energy Agency
Advanced Materials Research | Year: 2013

Surface hardening on local disc brakes with DC-plasma nitrocarburizing apparatus has been carried out. Local disc brake was nitrocarburized at temperatures of 700 °C, 800°C, and 900°C for 3 hours, respectively. The results show that the hardness of sample of local disc brake before nitrocarburization was 167.86 Kgf/mm2, after the sample was nitrocarburized at temperature 900 °C for 3 hours, the hardness increased up to 649.38 Kgf/mm2. Matrixes on the base material were austenite, ferrite, and perlite. © (2013) Trans Tech Publications, Switzerland. Source

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