Entity

Time filter

Source Type


Christianson S.,National Microbiology Laboratory Public Health Agency of Canada | Grierson W.,National Microbiology Laboratory Public Health Agency of Canada | Wolfe J.,National Microbiology Laboratory Public Health Agency of Canada | Sharma M.K.,National Microbiology Laboratory Public Health Agency of Canada | Sharma M.K.,University of Manitoba
Journal of Clinical Microbiology | Year: 2013

Macrolides are an important tool in the treatment of Mycobacterium avium complex infections. Here, we evaluate the use of 23S rRNA gene sequencing for the rapid detection of macrolide resistance. Routine sequencing of the 23S rRNA gene is highly specific for macrolide resistance but lacks in sensitivity. © 2013, American Society for Microbiology. Source


Golden A.R.,University of Manitoba | Rosenthal M.,University of Manitoba | Fultz B.,University of Manitoba | Nichol K.A.,Clinical Microbiology Health science Center | And 10 more authors.
Journal of Antimicrobial Chemotherapy | Year: 2015

Objectives: The goal of this study was to characterize Streptococcus pneumoniae demonstrating MDR (resistant to three or more antimicrobial classes) or XDR (resistant to five or more classes) phenotypes, collected from Canada during the CANWARD 2007-13 study. Methods: From 2007 to 2013 inclusive, S. pneumoniae isolates were collected as a part of the CANWARD surveillance study. MDR and XDR isolates were subjected to PFGE, MLST, molecular detection of pneumococcal pili and macrolide resistance determinants mef(A/E) and erm(B), sequencing of PBPs 1A, 2B and 2X and comparison with Pneumococcal Molecular Epidemiology Network (PMEN) clones. Results: Of 2129 S. pneumoniae isolates collected during the CANWARD 2007-13 study, 61 (2.9%) were found to be MDR. Of these MDR isolates, 43 (70.5%) were XDR. The most common serotypes for both MDR and XDR S. pneumoniae were 19A and 19F. Twenty-nine of 61 isolates (48%) demonstrated resistance to clarithromycin, clindamycin, doxycycline, penicillin and trimethoprim/sulfamethoxazole. All isolates possessed at least one macrolide resistance determinant and mutations in PBPs 1A, 2B and 2X. The most common clone was piliated, XDR ST320, an internationally circulating double-locus variant of Taiwan19F-14 (ST236). Conclusions: Though the rate of MDR S. pneumoniae has remained relatively stable since 2007, XDR strains have emerged in Canada. These strains are virulent, possess resistance determinants and are related to international clones. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. Source


Cruz J.,University of Alberta | Liu Y.,University of Alberta | Liang Y.,University of Alberta | Zhou Y.,University of Alberta | And 5 more authors.
Nucleic Acids Research | Year: 2012

Originally released in 2005, BacMap is an electronic, interactive atlas of fully sequenced bacterial genomes. It contains fully labeled, zoomable and searchable chromosome maps for essentially all sequenced prokaryotic (archaebacterial and eubacterial) species. Each map can be zoomed to the level of individual genes and each gene is hyperlinked to a richly annotated gene card. The latest release of BacMap (http://bacmap.wishartlab.com/) now contains data for more than 1700 bacterial species (∼10x more than the 2005 release), corresponding to more than 2800 chromosome and plasmid maps. All bacterial genome maps are now supplemented with separate prophage genome maps as well as separate tRNA and rRNA maps. Each bacterial chromosome entry in BacMap also contains graphs and tables on a variety of gene and protein statistics. Likewise, every bacterial species entry contains a bacterial 'biography' card, with taxonomic details, phenotypic details, textual descriptions and images (when available). Improved data browsing and searching tools have also been added to allow more facile filtering, sorting and display of the chromosome maps and their contents. © The Author(s) 2011. Source


Petkau A.,National Microbiology Laboratory Public Health Agency of Canada | Petkau A.,University of Manitoba | Stuart-Edwards M.,National Microbiology Laboratory Public Health Agency of Canada | Stuart-Edwards M.,University of Manitoba | And 3 more authors.
Bioinformatics | Year: 2010

Summary: GView is a Java application for viewing and examining prokaryotic genomes in a circular or linear context. It accepts standard sequence file formats and an optional style specification file to generate customizable, publication quality genome maps in bitmap and scalable vector graphics formats. GView features an interactive pan-and-zoom interface, a command-line interface for incorporation in genome analysis pipelines, and a public Application Programming Interface for incorporation in other Java applications. © The Author(s) 2010. Published by Oxford University Press. Source


Golden A.R.,University of Manitoba | Adam H.J.,University of Manitoba | Adam H.J.,Clinical Microbiology Health science Center | Gilmour M.W.,University of Manitoba | And 8 more authors.
Journal of Antimicrobial Chemotherapy | Year: 2014

Objectives: Serotype replacement in Streptococcus pneumoniae following the implementation of a new vaccine has been associated with the emergence of non-vaccine serotypes as prominent causes of invasive pneumococcal disease (IPD). The aim of this study was to characterize specific non-PCV-13 serotypes 15A, 22F, 33F and 35B from IPD, isolated in Canada post-PCV-13 introduction in 2010. Methods: Of 3802 IPD isolates collected from across Canada in 2011-13, 18.4% were found to be serotypes 15A, 22F, 33F and 35B. These 699 isolates were subjected to antimicrobial susceptibility testing, PFGE, MLST, molecular detection of pneumococcal pili and comparison with Pneumococcal Molecular Epidemiology Network (PMEN) clones. Results: This study demonstrated clonal spread of specific STs, including MDR ST63 and its Sweden. 15A-25-related variants, the increasingly common ST433 and a variant of piliated, penicillin-non-susceptible ST558, related to PMEN clone Utah. 35B-24 (ST377). New STs of serotype 33F were identified. Several potential capsular switching events were identified within these serotypes. Conclusions: Non-PCV-13 serotype 22F is increasing in Canada through the rapid clonal expansion of ST433. Numerous new STs associated with serotype 33F indicate the potential divergence of the serotype. Serotypes 15A and 35B in Canada are related to international clones of S. pneumoniae. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. Source

Discover hidden collaborations