National Marine Products Quality Supervision & Inspection Center

Zhanjiang, China

National Marine Products Quality Supervision & Inspection Center

Zhanjiang, China

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Lu P.,Guangdong Ocean University | Wu C.,Guangdong Ocean University | Shi Q.,National Marine Products Quality Supervision & Inspection Center | Wang Y.,Guangdong Ocean University | And 8 more authors.
Food Analytical Methods | Year: 2015

Accurate analyses of T-2 and HT-2 toxin in aquatic organisms including shrimp are important as these two toxins are increasingly detected in aquatic cereal-based feed. Therefore, the potential for these toxins to enter the human food chain from contaminated fish and shrimp products is very real. A rapid, sensitive, and validated method for simultaneous determination of T-2 and HT-2 toxins was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method following extraction of the two toxins from shrimp tissues with ethyl acetate. This method is simple in that additional solid-phase extraction is not required to isolate and purify the toxins. LC was performed on an analytical Hypersil GOLD column. The mobile phase consisted of methanol and 5 mM ammonium acetate containing 0.1 % formic acid. The MS/MS ion transitions for both the T-2 toxin (484.20 → 214.87) and HT-2 toxin (442.20 → 214.96) were monitored. And the most intense transition ion product (m/z) of T-2 and HT-2 used for quantification on the SRM mode of a mass spectrometer was 304.95 and 262.91, respectively. The results linearly correlated with coefficients >0.9990. The limits of quantification ranged from 0.02 to 0.51 ng·g−1 and from 0.17 to 4.48 ng·g−1 for T-2 and HT-2, respectively, depending on the shrimp tissue type. The overall extraction recovery for both toxins ranged between 84 and 111 % with RSD values less than 15.0 %, indicative of good replication. Furthermore, the recovery and precision levels were within the predefined limits (≤15 %) at all concentrations. The application of this method to study the accumulation of T-2 toxin in shrimp showed that it can be successfully used to monitor even very low tissue toxin concentrations. Research is in progress to extend this method for the measurement of T-2 and HT-2 in aquatic foods that enter the human food chain. © 2015 Springer Science+Business Media New York


Deng Q.,Guangdong Ocean University | Wang W.,Guangdong Ocean University | Sun L.,Guangdong Ocean University | Wang Y.,Guangdong Ocean University | And 5 more authors.
Analytical and Bioanalytical Chemistry | Year: 2016

Surfactin and iturin are antimicrobial lipopeptides produced from Bacillus spp. and have significant prospective applications in many fields. Therefore, accurate analysis of these lipopeptides in the fermented product of some Bacillus strains is important. A sensitive method for simultaneous quantitative determination of surfactin and iturin fermented by Bacillus natto NT-6 was developed and validated using liquid chromatography–tandem mass spectrometry. Crude extracts of antimicrobial lipopeptide samples were dissolved in a mixture of acetonitrile/water (7:3, v/v) in 0.1 % (v/v) formic acid and eluted with acetonitrile/water (7:3, v/v) containing 5 mmol L−1 ammonium acetate and 0.1 % (v/v) formic acid. The target compounds were detected by mass spectrometry (ESI+) using selective ion monitoring. A good linear regression in the range of 0.20–10.0 mg L−1 for both surfactin and iturin (R2 ≥ 0.9995) was observed with spiked recoveries of 93.3–108.2 %, RSD values less than 15 %, precision 4.14–13.30 %, and a detection limit of 0.374 mg L−1. This method has a simple preprocessing operation, good repeatability, and provides an accurate quantitative analysis of surfactin and iturin. [Figure not available: see fulltext.] © 2016 Springer-Verlag Berlin Heidelberg


PubMed | National Marine Products Quality Supervision & Inspection Center, Guangdong Ocean University and Lincoln University at Christchurch
Type: Journal Article | Journal: Analytical and bioanalytical chemistry | Year: 2016

Surfactin and iturin are antimicrobial lipopeptides produced from Bacillus spp. and have significant prospective applications in many fields. Therefore, accurate analysis of these lipopeptides in the fermented product of some Bacillus strains is important. A sensitive method for simultaneous quantitative determination of surfactin and iturin fermented by Bacillus natto NT-6 was developed and validated using liquid chromatography-tandem mass spectrometry. Crude extracts of antimicrobial lipopeptide samples were dissolved in a mixture of acetonitrile/water (7:3, v/v) in 0.1% (v/v) formic acid and eluted with acetonitrile/water (7:3, v/v) containing 5mmolL


Liang G.,Guangdong Ocean University | Pang H.,Guangdong Ocean University | Wang Y.,Guangdong Ocean University | Sun L.,Guangdong Ocean University | And 4 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2015

Two-dimensional electrophoresis (2-DE) method on proteome of Litopenaeus vannamei was established and optimized. The shrimp proteins were extracted by grinding in liquid nitrogen and following by ultrasonication, separated by 2-DE with the 7 cm IPG strip. Image analysis software was applied to analyze the 2-DE images after staining. Effect of the lysis buffer, protein extraction method, pH of IPG gel strip, loading protein amount and stain method on 2-DE images were detected. The results showed that the 2-DE pattern of shrimp protein with clear background and better resolution was successfully obtained using lysis buffer II, grinding in liquid nitrogen and following by ultrasonication, 7 cm pH 4-7 IPG gel strips, loading the 180 μg protein and dying the gels stained by blue silver. The study would provide a technical reference for the proteomic analysis and molecular marker identification of Litopenaeus vannamei muscle. © 2015, Chinese Institute of Food Science and Technology. All right reserved.

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