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Goto Y.,National Livestock Breeding Center Ohu Station Shichinohe | Hirayama M.,National Livestock Breeding Center Nishigou | Takeda K.,National Livestock Breeding Center Tokachi Station Otofuke | Tukamoto N.,National Livestock Breeding Center Tokachi Station Otofuke | And 3 more authors.
Animal Science Journal | Year: 2013

In this study, we compared the developmental ability of somatic cell nuclear transfer (SCNT) embryos reconstructed with three bovine somatic cells that had been synchronized in G0-phase (G0-SCNT group) or early G1-phase (eG1-SCNT group). Furthermore, we investigated the production efficiency of cloned offspring for NT embryos derived from these donor cells. The G0-phase and eG1-phase cells were synchronized, respectively, using serum starvation and antimitotic reagent treatment combined with shaking of the plate containing the cells (shake-off method). The fusion rate in the G0-SCNT groups (64.2±1.8%) was significantly higher than that of eG1-SCNT groups (39.2±1.9%) (P<0.05), but the developmental rates to the blastocyst stage of SCNT embryos per fused oocytes were similar for all groups. The overall production efficiency of the clone offspring in eG1-SCNT groups (12.7%) per recipient cow was higher than that in G0-SCNT groups (3%) (P<0.05). The mean birth weight of cloned calves and the average calving score in the G0-SCNT groups (48.1±3.4kg and 3.3±0.3, respectively) was significantly higher (P<0.05) than those of eG1-SCNT groups (37.2±2.1kg and 2.3±0.2, respectively). Results of this study indicate that synchronization of donor cells in eG1-phase using the shake-off method improved the overall production efficiency of the clone offspring per transferred embryo. © 2013 Japanese Society of Animal Science.

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