National Key Laboratory of Crop Biology

Tai’an, China

National Key Laboratory of Crop Biology

Tai’an, China
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An J.-P.,National Key Laboratory of Crop Biology | An J.-P.,MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation | An J.-P.,Shandong Agricultural University | Wang X.-N.,Shandong Agricultural University | And 15 more authors.
Plant and Soil | Year: 2017

Background and aims: Jasmonate (JA) and ethylene are involved in the regulation of the aluminum (Al)-induced growth inhibition. Although it has been reported that JA enhances Al-induced root-growth inhibition, its role in the regulation of growth interplaying with ethylene is still not well understood. In this study, we investigated the mechanism underlying the effect of apple MdMYC2 transcription factor on Al stress. Methods: Overexpression lines were used for functional analysis. Real-time quantitative RT-PCR was used to examine the expression level of ethylene responsive genes. ChIP-PCR, EMSA, and Y1H assays were used to test whether MdMYC2-GST fusion protein could directly bind to MdERF3 promoter. Transient transactivation assays in tobacco leave were conducted to confirm whether MdMYC2 positively regulated the expression of MdERF3. Results: MdMYC2 negatively regulated Al tolerance with up-regulating the expression of ethylene responsive genes. Moreover, MdMYC2 was observed to bind to the promoter of MdERF3, a positive regulator of ethylene biosynthesis, and directly activated its transcription. And applying the antagonist of ethylene biosynthesis, AVG, alleviated MdMYC2-modulated growth inhibition in Al stress. Conclusions: We consider that MdMYC2 protein directly interacts and promotes the transcript of MdERF3 to affect ethylene biosynthesis, thereby regulating the Al-mediated stress response. Our findings provide a deeper understanding of the crosstalk between JA and ethylene as well as JA-mediated growth inhibition in apple. © 2017 Springer International Publishing Switzerland


Ji X.-H.,National Key Laboratory of Crop Biology | Ji X.-H.,Apple Inc | Wang Y.-T.,National Key Laboratory of Crop Biology | Wang Y.-T.,Apple Inc | And 16 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2014

We have induced callus tissues from one R6/R6 homozygous genotype red-fleshed apple individual which was the hybrid offspring of Malus sieversii f.niedzwetzkyana and ‘Fuji’, and investigated the effect of auxin alone and auxin combined with cytokinin or nitrogen deficiency on anthocyanin synthesis. In callus culture, auxin alone significantly inhibited anthocyanin biosynthesis with the increase of auxin concentration. The inhibitory effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on anthocyanin accumulation was about tenfold stronger than naphthalene acetic acid. Anthocyanin regulatory genes (MdMYB10 and MdbHLH3) and structural genes were dramatically suppressed by 0.6 mg/L 2,4-D. The inhibitory effect of auxin on anthocyanin biosynthesis was influenced by cytokinins 6-benzylaminopurine (BAP) and thidiazuron (TDZ) as well as nitrogen deficiency. Auxin and cytokinin displayed the interaction in controlling anthocyanin biosynthesis. Co-treatment of auxin and cytokinin (BAP or TDZ) significantly enhanced the cytokinin-induced increase in anthocyanin levels but too high auxin concentration strongly inhibited anthocyanin synthesis even in the presence of cytokinin. Nitrogen deficiency could reverse the inhibition of anthocyanin accumulation by auxin. © 2014, Springer Science+Business Media Dordrecht.

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