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Zhang Z.,University of Connecticut | Zhang Z.,National Institutes for Food and Drug Control | Chen N.,University of Connecticut | Li S.,University of Connecticut | And 2 more authors.
Journal of the American Chemical Society | Year: 2012

The ability to regulate cell-material interactions is important in various applications such as regenerative medicine and cell separation. This study successfully demonstrates that the binding states of cells on a hydrogel surface can be programmed by using hybridized aptamers and triggering complementary sequences (CSs). In the absence of the triggering CSs, the aptamers exhibit a stable, hybridized state in the hydrogel for cell-type-specific catch. In the presence of the triggering CSs, the aptamers are transformed into a new hybridized state that leads to the rapid dissociation of the aptamers from the hydrogel. As a result, the cells are released from the hydrogel. The entire procedure of cell catch and release during the transformation of the aptamers is biocompatible and does not involve any factor destructive to either the cells or the hydrogel. Thus, the programmable hydrogel is regenerable and can be applied to a new round of cell catch and release when needed. © 2012 American Chemical Society. Source


Wang Y.-P.,National Institutes for Food and Drug Control
Chinese Journal of Biologicals | Year: 2012

Objective: To evaluate the effect of aluminum hydroxide adjuvant on cellular immune response induced with inactivated enterovirus 71 (EV71) in mice. Methods: BALB/c mice were immunized with aluminum hydroxide adjuvant-containing and adjuvant-free inactivated EV71 vaccines respectively, using physiological saline as control. Blood samples were collected 7 d after booster immunization, from which mononuclear cells (MNCs) were isolated and CD8+ T cells were removed. The secretion level of IFNγ by mouse splenic MNCs after stimulation with bulk EV71 vaccine or VPI-36 was determined by ELISPOT, while those of IL-2, IL-4, IL-5, IL-6 and IL-10 by LUMINEX technique. The serum neutralizing antibody titers of mice were determined by micro-neutralization test in vitro. Results: The number of spot-forming cells (SFCs) of IFNγ secreted by MNCs of BALB/c mice immunized twice with aluminum hydroxide adjuvant -containing EV71 vaccine were significantly higher than those with adjuvant-free vaccine (P < 0.05). Both the positive conversion rates of neutralizing antibody against EV71 in sera of mice immunized with aluminum hydroxide adjuvant-containing and adjuvant-free vaccines were 100%. However, the neutralizing antibody titer induced by aluminum hydroxide adjuvant-containing was significantly higher than that by adjuvant-free vaccine (P < 0.01). Conclusion: Inactivated EV71 vaccine induced secretion of specific IFNγ, IL-2, IL-6 and IL-10, and aluminum hydroxide adjuvant enhanced Th1 and Th2 immune responses induced by inactivated EV71 vaccine. Source


He P.,National Institutes for Food and Drug Control
Chinese Journal of Biologicals | Year: 2011

Objective: To investigate the antigen-specific cytokine responses induced by various kinds of hepatitis B (HB) vaccines in mice as well as the TH1 cytokine-secreting ability of mononuclear cells, and evaluate the immunogenicities of the vaccines. Methods: BALB/c mice (H-2 d) were immunized with recombinant Hansenula polymorpha (HP)-, Saccharomyces cerevisiae (SC)- and CHO cells-derived HB vaccines respectively. The splenic mononuclear cells (MNCs) of mice were isolated 4, 7, 10, 14, 25 and 35 d after immunization respectively, stimulated with HBsAg in vitro, and determined for IFN-γ, IL-2 and TNF-α levels by Luminex method. Results: The IFN-γ and TNF-α levels of mice induced by the three kinds of HB vaccine reached peak values 10 d after immunization then decreased, while the IL-2 level increased gradually. The IFNγ levels 10 and 14 d after immunization with SC-derived vaccine (P < 0.01), the IL-2 levels 10, 25 and 35 d after immunization with HP-derived vaccine (P < 0.05), the TNF-α levels 10, 14 and 35 d after immunization with SC-derived vaccine(P < 0.05) as well as the TNF-α levels 7 and 25 d after immunization with HP-derived vaccine (P < 0.05) were significantly higher than those with CHO cells-derived vaccine. Conclusion: The levels and changing tendencies of cellular immune responses in mice induced by various kinds of HB vaccine were different. The TH1 cytokine level induced by yeast-derived vaccine was higher than that by CHO cells-derived vaccine. Source


Zhao Y.-Y.,Northwest University, China | Zhao Y.-Y.,University of California at Irvine | Cheng X.-L.,National Institutes for Food and Drug Control | Lin R.-C.,Beijing University of Chinese Medicine
International Review of Cell and Molecular Biology | Year: 2014

Lipids are the fundamental components of biological membranes as well as the metabolites of organisms. Lipids play diverse and important roles in biologicals. The lipid imbalance is closely associated with numerous human lifestyle-related diseases, such as atherosclerosis, obesity, diabetes, and Alzheimer's disease. Lipidomics or lipid profiling is a system-based study of all lipids aiming at comprehensive analysis of lipids in the biological system. Lipidomics has been accepted as a lipid-related research tool in lipid biochemistry, clinical biomarker discovery, disease diagnosis, and in understanding disease pathology. Lipidomics will not only provide insights into the specific functions of lipid species in health and disease, but will also identify potential biomarkers for establishing preventive or therapeutic programs for human diseases. This review presents an overview of lipidomics followed by in-depth discussion of its application to the study of human diseases, including extraction methods of lipids, analytical technologies, data analysis, and clinical research in cancer, neuropsychiatric disease, cardiovascular disease, kidney disease, and respiratory disease. We describe the current status of the identification of metabolic biomarkers in different diseases. We also discuss the lipidomics for the future perspectives and their potential problems. The application of lipidomics in clinical studies may provide new insights into lipid profiling and pathophysiological mechanisms. © 2014 Elsevier Inc. Source


Zhang S.-Q.,National Institutes for Food and Drug Control
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2011

A sensitive and selective liquid chromatographic-tandem mass spectrometric method was developed for quantification of triamcinolone acetonide (TA) in rabbit ocular tissues. After a simple liquid-liquid extraction using tert-butyl methyl ether, TA and internal standard methylprednisolone were separated on a C 18 column with a mobile phase of acetonitrile:water:formic acid (60:40:0.1, v/v/v) and quantified by the use of selected reaction monitoring mode with a total run time of 4min. The method was validated in tissue homogenates with a daily working range of 1-1000ng/mL with correlation coefficient of more than 0.99 and a sensitivity of 1ng/mL as lower limit of quantification, respectively. The mean intra-day and inter-day precisions were less than 10% and accuracy values were higher than 90%. This method was fully validated for the accuracy, precision and stability studies. The method proved to be accurate and specific, and was applied to an in vivo biodistribution study of TA after intravitreal injection to rabbits. Values of mean residence time in vitreous humor, crystalline lens and aqueous humor were 27.7, 35.8 and 20.0 days, respectively. © 2011 Elsevier B.V. Source

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