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Szita G.,Szent Istvan University | Bernath S.,National Food Chain Safety Office | Szili Z.,Szent Istvan University | Szita J.,National Institute of Traumatology and Emergency Medicine | And 2 more authors.
Acta Alimentaria | Year: 2014

A selective synthetic solid minimal medium (BS agar) was developed to detect antimicrobial drug-residues in foodstuffs using Bacillus subtilis indicator culture. This medium contains an ammonium salt as nitrogen source and either glucose or sodium pyruvate as carbon sources.Its selectivity is based on the fact that Bacillus subtilis is still able to grow if the minimal medium consists of simple inorganic substances as nitrogen sources, and glucose or pyruvate as carbon supply. Using these new synthetic media for microbiological assays assessing certain antimicrobials, the diameter of the inhibition zones were 1.4-4 times wider than on the Mueller-Hinton agar.The advantages of the BS agars are their standard compositions, the absence of inhibitors, the reproducible quality and the low costs.

Vendegh Z.,National Institute of Traumatology and Emergency Medicine | Melly A.,National Institute of Traumatology and Emergency Medicine | Toth B.,National Institute of Traumatology and Emergency Medicine | Wolf K.,Krankenhaus Munich Schwabing | And 3 more authors.
Clinical Hemorheology and Microcirculation | Year: 2010

Different bones have different blood supplies, which may influence bone healing. Therefore, elucidation of the mechanisms involved in the regulation of bone marrow blood flow in different bones is of high clinical importance. To assess the micro circulation of bone marrow of the femur and tibia simultaneously, flow velocities were continuously measured by a two-channel laser-Doppler flowmeter. The probes were introduced into the femoral and tibial diaphysis, respectively, in the anesthetized rabbit. Changes in micro circulation of the bone marrow were elicited by intra-arterial bolus injections of vasoactive substances: epinephrine (E), calcitonine-gene related peptide (CGRP), substance P (SP), sodium nitroprusside (SNP), E and Ebrantil. Systemic arterial blood pressure was recorded with an electro-manometer. Micro vascular resistance (MVR) and 50% recovery time (50RT) to baseline flow level were calculated from the measured data. Flow velocity in the femur was significantly higher. Epinephrine considerably reduced micro vascular blood flow, which could be significantly warded off by Ebrantil. CGRP and SP did not change MVR. Application of SNP resulted in reduction of flow velocity, but it also decreased MVR. No statistically significant differences were found between reactions of the micro circulation in the two marrows. These results suggest that there are no significant differences between the blood flow response patterns of these two bone marrow sites, thus the regulation patterns of the micro circulation of the two bones are also similar. © 2010 - IOS Press and the authors. All rights reserved.

Kiss R.,A.L. Instruments | Szita G.,Szent Istvan University | Herpay M.,National Center for Epidemiology | Csiko Gy.,Szent Istvan University | And 8 more authors.
Acta Alimentaria | Year: 2011

The authors investigated the possibility of the presence of VTEC strains in improperly pasteurized milk samples. A total of 64 Escherichia coli strains were isolated from 135 pasteurized milk samples originating from the same producer. The examined isolates contained 29 haemolysin-, 9 colicin- and 5 aerobactin-producing strains, but the investigations concerning heat-resistant and heat-sensitive toxins gave negative results.Six O128-type E. coli strains exerted a cytotoxic effect on the VERO cell line; 5 of them contained H12 antigen, while one could not be typed. Four of the 6 verocytotoxin-producing strains belonged in phage group 20, one in phage group (2)3(7), and one in phage group 4; four strains were of B3, one of A1, and one of A1(A2) phage type.Because of a technical failure the milk was pasteurized at 69°C for 15 s, which is 2°C less than required. The results underline the importance of the appropriate pasteurization temperature, as otherwise the milk may contain verocytotoxin-producing E. coli, which is a potentially great hazard for public health.

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