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Yajima Y.,Japan National Institute of Advanced Industrial Science and Technology | Inaba S.,National Institute of Technology and Evaluation NITE | Degawa Y.,University of Tsukuba | Hoshino T.,Japan National Institute of Advanced Industrial Science and Technology | Kondo N.,Hokkaido University

This is the first report on ultrastructural observations of nivicolous myxomycete fruiting bodies with cyst-like bodies. One specimen had immature fruiting bodies of a Lamproderma sp. just after spore cleavage. Using light microscopy and scanning and transmission electron microscopy, countless cyst-like bodies were observed inside of the developing spores, capillitium, columella and stalk. A second specimen consisted of mature fruiting bodies of L. echinosporum. Outward appearance of the fruiting bodies was normal with numerous cyst-like bodies inside the entire fruiting body. The cyst-like bodies in both myxomycete specimens have a common morphology: 5-6 |un in diam, being transparent under light microscopy and densely waited by SEM and TEM observation. The partial sequence of the 18S rDNA gene of the first specimen suggested that cyst-like bodies were in the Cryptomycota (including Rozellida), placed at the root of a fungal clade. Source

Gleason F.H.,University of Sydney | Marano A.V.,National University of La Plata | Digby A.L.,University of Sydney | Al-Shugairan N.,University of Sydney | And 5 more authors.

A phylogenetically diverse set of seventeen isolates of Chytridiomycota were selected for a study of the utilization of common carbohydrates as sole carbon sources in synthetic media. Rhizophlyctis rosea AUS 13 is capable of the digestion of crystalline cellulose in the form of lens paper, filter paper and powdered filter paper and grows well with noncrystaline carboxymethyl cellulose or cellobiose, but cannot use starch or maltose as sole carbon sources in liquid and on solid media. None of the other sixteen isolates tested can digest crystalline cellulose, but all grow well on starch and maltose and several can also use cellobiose and/or sucrose as a sole carbon source. Four of the other sixteen isolates could also digest carboxymethyl cellulose slowly. Glucose is an excellent sole source of carbon in synthetic media for all seventeen isolates in the present study. In general, these data suggest variability in the ability of zoosporic true fungi to use carbohydrates other than glucose as sole sources of carbon. Four patterns of carbohydrate utilization emerged from this study of seventeen isolates. R. rosea degrades cellulose over a relatively wide pH range which suggests that the cellulase enzymes are stable over a wide pH range. © Springer Science+Business Media B.V. 2010. Source

Imai H.,Japan National Institute of Advanced Industrial Science and Technology | Imai H.,National Institute of Technology and Evaluation NITE
Joint IMEKO TC11-TC19-TC20 Int. Symp. Metrological Infrastructure, Environmental and Energy Measurement and Int. Symp. of Energy Agencies of Mediterranean Countries, IMEKO-MI 2011

Recent situation on global metrology is reviewed from the view point of requirements of science and technology. Basic infrastructure on quantities and units is built up under the international recognition and rules in science of measurement. Metrological traceability to a metrological reference such as a measurement unit, a reference procedure, and a reference material is considered to be fundamentals in calibration and measurement. In order to confirm the reliability of a measurement result, statement of metrological traceability chain together with attributed measurement uncertainty is required for calibration and testing laboratories. Concepts and definitions of terms are documented in such as SI brochure, VIM, GUM and some other standard documents of ISO, IEC, OIML and ILAC. In Mutual Recognition Arrangement (MRA) such as CIPM and ILAC, statements of competence for both quality management system and technology based on ISO/IEC 17025 [1] are required. Detailed description on metrological traceability and measurement uncertainty for a measurement result is also required in many fields of science, industry and our social life from the safety point of view. Source

Ukida A.,National Hospital Organization Okayama Medical Center | Ukida A.,Okayama University of Science | Yamasaki O.,National Hospital Organization Okayama Medical Center | Yamasaki O.,Okayama University of Science | And 2 more authors.
Nishinihon Journal of Dermatology

A 39-year-old man was admitted to the department of cardiovascular surgery for an operation of lower limbs varication. Four days after the admission, itchy erythematous edematous rash appeared on his face, neck and elbow, and became enlarged. Positive reactions to patch testing were induced by the pajamas. We analyzed the pajamas and obtained samples of textile dyes used for polyester. The patient reacted positively to Disperse Blue 106 and 124. We diagnosed his allergy as contact dermatitis induced by disperse dyes similar to Disperse Blue 106 and Disperse Blue 124. When a toxic rash occurs during hospitalization, we should be aware of a possible occurrence of contact dermatitis due to the patient's pajamas. Source

Yukphan P.,National Center for Genetic Engineering and Biotechnology | Malimas T.,National Center for Genetic Engineering and Biotechnology | Muramatsu Y.,National Institute of Technology and Evaluation NITE | Potacharoen W.,National Center for Genetic Engineering and Biotechnology | And 6 more authors.
Bioscience, Biotechnology and Biochemistry

Isolates AH11T and AH13T were isolated from flowers of lantana and candle bush respectively collected in Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates formed an independent cluster, which was then connected to the type strain of Saccharibacter floricola. The calculated pair-wise 16S rRNA gene sequence similarities of isolate AH11T were 95.7-92.3% to the type strains of the type species of the 12 genera of acetic acid bacteria. The DNA base composition was from 51.2 to 56.8mol% G+C, with a range of 5.6 mol %. When isolate AH11T was labeled, DNA-DNA similarities were 100, 12, 4, 5, and 4% respectively to isolates AH11T and AH13T and the type strains of Saccharibacter floricola, Gluconobacter oxydans, and Acetobacter aceti. The two isolates were non-motile and did not oxidize either acetate or lactate. No growth was found in the presence of 0.35% acetic acid w/v. The two isolates were not osmophilic but osmotolerant, produced 2,5-diketo-D-gluconate from D-glucose, and did not oxidize lactate, thus differing from strains of Saccharibacter floricola, which showed weak lactate oxidation. The two isolates contained unsaturated C18:1ω7c fatty acid as the major fatty acid, and were unique in the presence of a considerable amount of straight-chain C18:12OH fatty acid. Q-10 was present as the major isoprenoid quinone. Neokomagataea gen. nov. was proposed with the two species, Neokomagataea thailandica sp. nov. for isolate AH11T (=BCC 25710 T = NBRC 106555T), which has 56.8 mol% G+C, and Neokomagataea tanensis sp. nov. for isolate AH13T (=BCC 25711 T = NBRC 106556T), which has 51.2 mol% G+C. Source

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