National Institute of Technology and Evaluation NITE

Kisarazu, Japan

National Institute of Technology and Evaluation NITE

Kisarazu, Japan
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Ito K.,Nippon Steel & Sumitomo Metal Corporation | Uchiyama T.,National Institute of Technology and Evaluation NITE | Iino T.,National Institute of Technology and Evaluation NITE | Mori K.,National Institute of Technology and Evaluation NITE | And 4 more authors.
Zairyo to Kankyo/ Corrosion Engineering | Year: 2010

Iron corrosion under enrichment culture of anaerobic microorganisms utilizing metallic iron as an electron donor was investigated. Enrichment culture from residual water on bottom of a crude oil storage tank in Kyushu region caused severe iron corrosion. But the other two enrichment cultures from drain water of an oil field in Tohoku region and sediment at mouth of the Edogawa river did not corrode iron severely. Black ferrous sulfide film was detected in the corrosion products under the enrichment cultures from the drain water of the oil field in Tohoku region and the sediment at mouth of the Edogawa river. Therefore, sulfate reducing bacteria (SRB) were considered to be causative microorganisms to iron corrosion. But the ferrous sulfide film was not detected in the corrosion products under the enrichment culture from the crude oil storage tank in Kyushu region. Ferrous carbonate (FeCO3) was a main component of the corrosion products by the enrichment culture from the crude oil storage tank in Kyushu region. Therefore, iron corrosion under the enrichment culture from the crude oil storage tank in Kyushu region was considered to be not simply caused by the SRB. Open circuit potential of a carbon steel (SS400) coupon with corrosion products under the enrichment culture from the crude oil storage tank in Kyushu region was about 120 to 140 mV higher than that by the other two enrichment cultures or abiotic control in anaerobic artificial seawater deaerated by Ar gas. Therefore, Galvanic coupling between corrosion products layer and base metal was considered to be possible mechanism of iron corrosion under the enrichment culture of the crude oil storage tank in Kyushu region.


Ukida A.,National Hospital Organization Okayama Medical Center | Ukida A.,Okayama University of Science | Yamasaki O.,National Hospital Organization Okayama Medical Center | Yamasaki O.,Okayama University of Science | And 3 more authors.
Nishinihon Journal of Dermatology | Year: 2014

A 39-year-old man was admitted to the department of cardiovascular surgery for an operation of lower limbs varication. Four days after the admission, itchy erythematous edematous rash appeared on his face, neck and elbow, and became enlarged. Positive reactions to patch testing were induced by the pajamas. We analyzed the pajamas and obtained samples of textile dyes used for polyester. The patient reacted positively to Disperse Blue 106 and 124. We diagnosed his allergy as contact dermatitis induced by disperse dyes similar to Disperse Blue 106 and Disperse Blue 124. When a toxic rash occurs during hospitalization, we should be aware of a possible occurrence of contact dermatitis due to the patient's pajamas.


Nagata Y.,Tohoku University | Natsui S.,Tohoku University | Endo R.,Tohoku University | Ohtsubo Y.,Tohoku University | And 6 more authors.
Enzyme and Microbial Technology | Year: 2011

The complete genome sequencing of a γ-hexachlorocyclohexane-degrading strain, Sphingobium japonicum UT26, revealed that the genome consists of two circular chromosomes [with sizes of 3.5 Mb (Chr1) and 682. kb (Chr2)], a 191-kb large plasmid (pCHQ1), and two small plasmids with sizes of 32 and 5. kb. The lin genes are dispersed on Chr1, Chr2, and pCHQ1. Comparison of the UT26 genome with those of other sphingomonad strains demonstrated that the " specific" lin genes for conversion of γ-HCH to β-ketoadipate (linA, linB, linC, linRED, and linF) are located on the DNA regions unique to the UT26 genome, suggesting the acquisition of these lin genes by horizontal transfer events. On the other hand, linGHIJ and linKLMN are located on the regions conserved in the genomes of sphingomonads, suggesting that the linGHIJ-encoded β-ketoadipate pathway and the LinKLMN-type ABC transporter system are involved in core functions of sphingomonads. Based on these results, we propose a hypothesis that UT26 was created by recruiting the specific lin genes into a strain having core functions of sphingomonads. Most of the specific lin genes in UT26 are associated with IS. 6100. Our analysis of spontaneous linA-, linC-, and linRED-deletion mutants of UT26 revealed the involvement of IS. 6100 in their deduced genome rearrangements. These facts strongly suggest that IS. 6100 plays important roles both in the dissemination of the specific lin genes and in the genome rearrangements. © 2011 Elsevier Inc.


PubMed | Kyoto Prefectural University of Medicine, National Institute of Technology and Evaluation NITE and Kyoto University
Type: Journal Article | Journal: Journal of rural medicine : JRM | Year: 2015

The objective of this study was to verify the recognition of dangers and obstacles within a house in the elderly when walking based on analyses of gaze point fixation.The rate of recognizing indoor dangers was compared among 30 elderly, 14 middle-aged and 11 young individuals using the Eye Mark Recorder.1) All of the elderly, middle-aged and young individuals showed a high recognition rate of 100% or near 100% when ascending outdoor steps but a low rate of recognizing obstacles placed on the steps. They showed a recognition rate of about 60% when descending steps from residential premises to the street. The rate of recognizing middle steps in the elderly was significantly lower than that in younger and middle-aged individuals. Regarding recognition indoors, when ascending stairs, all of the elderly, middle-aged and young individuals showed a high recognition rate of nearly 100%. When descending stairs, they showed a recognition rate of 70-90%. However, although the recognition rate in the elderly was lower than in younger and middle-aged individuals, no significant difference was observed. 2) When moving indoors, all of the elderly, middle-aged and young individuals showed a recognition rate of 70%-80%. The recognition rate was high regarding obstacles such as floors, televisions and chests of drawers but low for obstacles in the bathroom and steps on the path. The rate of recognizing steps of doorsills forming the division between a Japanese-style room and corridor as well as obstacles in a Japanese-style room was low, and the rate in the elderly was low, being 40% or less.The rate of recognizing steps of doorsills as well as obstacles in a Japanese-style room was lower in the elderly in comparison with middle-aged or young individuals.


Yukphan P.,National Center for Genetic Engineering and Biotechnology | Malimas T.,National Center for Genetic Engineering and Biotechnology | Muramatsu Y.,National Institute of Technology and Evaluation NITE | Potacharoen W.,National Center for Genetic Engineering and Biotechnology | And 6 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2011

Isolates AH11T and AH13T were isolated from flowers of lantana and candle bush respectively collected in Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates formed an independent cluster, which was then connected to the type strain of Saccharibacter floricola. The calculated pair-wise 16S rRNA gene sequence similarities of isolate AH11T were 95.7-92.3% to the type strains of the type species of the 12 genera of acetic acid bacteria. The DNA base composition was from 51.2 to 56.8mol% G+C, with a range of 5.6 mol %. When isolate AH11T was labeled, DNA-DNA similarities were 100, 12, 4, 5, and 4% respectively to isolates AH11T and AH13T and the type strains of Saccharibacter floricola, Gluconobacter oxydans, and Acetobacter aceti. The two isolates were non-motile and did not oxidize either acetate or lactate. No growth was found in the presence of 0.35% acetic acid w/v. The two isolates were not osmophilic but osmotolerant, produced 2,5-diketo-D-gluconate from D-glucose, and did not oxidize lactate, thus differing from strains of Saccharibacter floricola, which showed weak lactate oxidation. The two isolates contained unsaturated C18:1ω7c fatty acid as the major fatty acid, and were unique in the presence of a considerable amount of straight-chain C18:12OH fatty acid. Q-10 was present as the major isoprenoid quinone. Neokomagataea gen. nov. was proposed with the two species, Neokomagataea thailandica sp. nov. for isolate AH11T (=BCC 25710 T = NBRC 106555T), which has 56.8 mol% G+C, and Neokomagataea tanensis sp. nov. for isolate AH13T (=BCC 25711 T = NBRC 106556T), which has 51.2 mol% G+C.


Gleason F.H.,University of Sydney | Marano A.V.,National University of La Plata | Digby A.L.,University of Sydney | Al-Shugairan N.,University of Sydney | And 5 more authors.
Hydrobiologia | Year: 2011

A phylogenetically diverse set of seventeen isolates of Chytridiomycota were selected for a study of the utilization of common carbohydrates as sole carbon sources in synthetic media. Rhizophlyctis rosea AUS 13 is capable of the digestion of crystalline cellulose in the form of lens paper, filter paper and powdered filter paper and grows well with noncrystaline carboxymethyl cellulose or cellobiose, but cannot use starch or maltose as sole carbon sources in liquid and on solid media. None of the other sixteen isolates tested can digest crystalline cellulose, but all grow well on starch and maltose and several can also use cellobiose and/or sucrose as a sole carbon source. Four of the other sixteen isolates could also digest carboxymethyl cellulose slowly. Glucose is an excellent sole source of carbon in synthetic media for all seventeen isolates in the present study. In general, these data suggest variability in the ability of zoosporic true fungi to use carbohydrates other than glucose as sole sources of carbon. Four patterns of carbohydrate utilization emerged from this study of seventeen isolates. R. rosea degrades cellulose over a relatively wide pH range which suggests that the cellulase enzymes are stable over a wide pH range. © Springer Science+Business Media B.V. 2010.


Yajima Y.,Japan National Institute of Advanced Industrial Science and Technology | Inaba S.,National Institute of Technology and Evaluation NITE | Degawa Y.,University of Tsukuba | Hoshino T.,Japan National Institute of Advanced Industrial Science and Technology | Kondo N.,Hokkaido University
Karstenia | Year: 2013

This is the first report on ultrastructural observations of nivicolous myxomycete fruiting bodies with cyst-like bodies. One specimen had immature fruiting bodies of a Lamproderma sp. just after spore cleavage. Using light microscopy and scanning and transmission electron microscopy, countless cyst-like bodies were observed inside of the developing spores, capillitium, columella and stalk. A second specimen consisted of mature fruiting bodies of L. echinosporum. Outward appearance of the fruiting bodies was normal with numerous cyst-like bodies inside the entire fruiting body. The cyst-like bodies in both myxomycete specimens have a common morphology: 5-6 |un in diam, being transparent under light microscopy and densely waited by SEM and TEM observation. The partial sequence of the 18S rDNA gene of the first specimen suggested that cyst-like bodies were in the Cryptomycota (including Rozellida), placed at the root of a fungal clade.


Imai H.,Japan National Institute of Advanced Industrial Science and Technology | Imai H.,National Institute of Technology and Evaluation NITE
Joint IMEKO TC11-TC19-TC20 Int. Symp. Metrological Infrastructure, Environmental and Energy Measurement and Int. Symp. of Energy Agencies of Mediterranean Countries, IMEKO-MI 2011 | Year: 2011

Recent situation on global metrology is reviewed from the view point of requirements of science and technology. Basic infrastructure on quantities and units is built up under the international recognition and rules in science of measurement. Metrological traceability to a metrological reference such as a measurement unit, a reference procedure, and a reference material is considered to be fundamentals in calibration and measurement. In order to confirm the reliability of a measurement result, statement of metrological traceability chain together with attributed measurement uncertainty is required for calibration and testing laboratories. Concepts and definitions of terms are documented in such as SI brochure, VIM, GUM and some other standard documents of ISO, IEC, OIML and ILAC. In Mutual Recognition Arrangement (MRA) such as CIPM and ILAC, statements of competence for both quality management system and technology based on ISO/IEC 17025 [1] are required. Detailed description on metrological traceability and measurement uncertainty for a measurement result is also required in many fields of science, industry and our social life from the safety point of view.


PubMed | National Institute of Technology and Evaluation NITE
Type: Journal Article | Journal: Microbes and environments | Year: 2011

A series of additives were evaluated for their effects on improving the yield and quality of DNA extracted from recalcitrant soils. Levels of possible DNA contaminants in these supplements were also assessed. Three of the additives (skim milk, casein, and RNA) were shown to be effective in improving the stable extraction of DNA from recalcitrant samples of Andosol. However, whereas skim milk appeared to be the most effective additive for this purpose, our data indicated that this commercially sourced product contained considerable amounts of contaminant DNA (30 to 40 g/g skim milk). A ribosomal intergenic spacer analysis (RISA) revealed the consistent contamination of different batches of this product with DNA of several species of both eukaryotes (cattle and protists) and prokaryotes. In particular, thermophilic bacteria such as Geobacillus and Anoxybacillus were identified in the sequenced PCR amplicons from skim milk. The results of the RISA clearly also indicated that the impact of contaminated DNA on the analysis of a microbial community could be significant when skim milk is used for extracting DNA from a recalcitrant soil. In contrast, only a trace amount of contaminating DNA was evident in casein and none was detected in the RNA examined in the present study.


PubMed | National Institute of Technology and Evaluation NITE
Type: Journal Article | Journal: The Journal of general and applied microbiology | Year: 2011

Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct novel species of the ascomycetous anamorphic yeast genus Candida. These strains are located in the Clavispora-Metschnikowia clade in a phylogenetic tree based on the D1/D2 domain sequences of the large subunit rRNA genes. Together with Candida picinguabensis and Candida saopaulonensis, the four novel species constitute a well-separated subclade from other species of the Clavispora-Metschnikowia clade. Three species from Thailand are described as Candida bambusicola sp. nov. (type strain, ST-50(T) = BCC 7750(T) = NBRC 106734(T) = CBS 11723(T)), Candida nongkhaiensis sp. nov. (type strain, ST-95(T) = BCC 8331(T) = NBRC 105874(T) =CBS 11724(T)) and Candida succicola sp. nov. (type strain, ST-631(T) = BCC 15314(T) = NBRC 106736(T) = CBS 11726(T)), respectively, and the species from Taiwan is described as Candida touchengensis sp. nov. (type strain, SY4S03(T) = NBRC 102647(T) = BCRC 23097(T) = CBS 10585(T)).

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