Placa J.R.,National Institute of Science and Technology in Stem Cell |
Placa J.R.,University of Sao Paulo |
Bueno R.D.B.E.L.,University of Sao Paulo |
Bueno R.D.B.E.L.,National Institute of Science and Technology in Stem Cell |
And 8 more authors.
Genomics Data | Year: 2015
Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignancies of the head and neck tumors Zhang et al., 2013 ). Previous studies have associated its occurrence with social activities, such as tobacco and alcohol consumption (Hashibe et al., 2007a ; Hashibe et al., 2007b ; Shangina et al., 2006 ). Here, we performed a genome-wide gene expression profiling in thirty-one patients positively diagnosed for LSCC, in order to investigate new targets involved in tumorigenesis. © 2015.
Alves C.P.,Centro Regional Of Hemoterapia Of Ribeirao Preto |
Alves C.P.,National Institute of Science and Technology in Stem Cell |
Alves C.P.,University of Sao Paulo |
Fonseca A.S.,Centro Regional Of Hemoterapia Of Ribeirao Preto |
And 21 more authors.
Stem Cells | Year: 2013
Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-b1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-b1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD1331/CD441) presents much higher levels of Hotair when compared with the non-stem cell subpopulation. These results indicate that Hotair acts as a key regulator that controls the multiple signaling mechanisms involved in EMT. Altogether, our data suggest that the role of Hotair in tumorigenesis occurs through EMT triggering and stemness acquisition. © AlphaMed Press.