Cantacuzino National Institute of Research Development for Microbiology and Immunology

Bucharest, Romania

Cantacuzino National Institute of Research Development for Microbiology and Immunology

Bucharest, Romania

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Dinu S.,Cantacuzino National Institute of Research Development for Microbiology and Immunology | Guillot S.,Institute Pasteur Paris | Dragomirescu C.C.,Cantacuzino National Institute of Research Development for Microbiology and Immunology | Dragomirescu C.C.,Carol Davila University of Medicine and Pharmacy | And 6 more authors.
Diagnostic Microbiology and Infectious Disease | Year: 2014

The incidence of whooping cough in Romania is substantially underestimated, and, as noted by the health authorities, this is mostly due to the lack of both awareness and biological diagnosis. We conducted a 1-year study in Bucharest in order to assess the circulation of Bordetella pertussis, the main etiological agent of whooping cough. Fifty-one subjects suspected of whooping cough were enrolled. Culture, real-time PCR, and enzyme-linked immunosorbent assay were used for laboratory diagnosis. Whooping cough patients (63%) were distributed among all age groups, and most were unvaccinated, incompletely vaccinated, or had been vaccinated more than 5 years previously. Bordetella holmesii DNA was detected in 22% of the bordetellosis cases; these patients included adults; teenagers; and, surprisingly, young children. B. pertussis isolates were similar to the clinical isolates currently circulating elsewhere in Europe. One isolate does not express pertactin, an antigen included in some acellular pertussis vaccines. © 2014 Elsevier Inc.


Lixandru B.E.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2010

The currative properties of aromatic and medicinal plants have been recognized since ancient times and, more recently, the antimicrobial activity of plant essential oils has been used in several applications, including food preservation. The purpose of this study was to create directly comparable, quantitative data on the antimicrobial activity of some plant essential oils prepared in the National Institute of Research-Development for Chemistry and Petrochemistry, Bucharest to be used for the further development of food packaging technology, based on their antibacterial and antifungal activity. The essential oils extracted from thyme (Thymus vulgaris L.), basil (Ocimum basilicum L.), coriander (Coriandrum sativum L.), rosemary (Rosmarinus officinalis L.), sage (Salvia officinalis L.), fennel (Foeniculum vulgare L.), spearmint (Mentha spicata L.) and carraway (Carum carvi L.) were investigated for their antimicrobial activity against eleven different bacterial and three fungal strains belonging to species reported to be involved in food poisoning and/or food decay: S. aureus ATCC 25923, S. aureus ATCC 6538, S. aureus ATCC 25913, E. coli ATCC 25922, E. coli ATCC 35218, Salmonella enterica serovar Enteritidis Cantacuzino Institute Culture Collection (CICC) 10878, Listeria monocytogenes ATCC 19112, Bacillus cereus CIP 5127, Bacillus cereus ATCC 11778, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404, Penicillium spp. CICC 251 and two E. coli and Salmonella enterica serovar Enteritidis clinical isolates. The majority of the tested essential oils exibited considerable inhibitory capacity against all the organisms tested, as supported by growth inhibition zone diameters, MICs and MBC's. Thyme, coriander and basil oils proved the best antibacterial activity, while thyme and spearmint oils better inhibited the fungal species.


Costache A.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2013

Cyclic AMP (cAMP), one of the most important secondary messengers, is produced by adenylate cyclase (AC) from adenosine triphosphate (ATP). AC is a widespread enzyme, being present both in prokaryotes and eukaryotes. Although they have the same enzymatic activity (ATP cyclization), the structure of these proteins varies, depending on their function and the producing organism. Some pathogenic bacteria utilize these enzymes as toxins which interact with calmodulin (or another eukaryote activator), causing intense cAMP synthesis and disruption of infected cell functions. In contrast, other pathogenic bacteria benefit of augmentation of AC activity for their own function. Based on sequence analysis ofAC catalytic domain from two pathogenic bacteria (Bacillus anthracis and Bordetellapertussis) with known three-dimensional structures, a possible secondary structure for 1-255 amino acid fragment from Pseudomonas aeruginosa AC (with 80TKGFSVKGKSS90 as the ATP binding site) is proposed.


Giuca M.C.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2011

Ten Haemophilus influenzae strains were isolated from patients aged between 1.6 - 24 years, with various diagnoses (acute meningitis, acute upper respiratory infection, otitis media and acute sinusitis). Identification was based on phenotypic and molecular characteristics; antibiotic susceptibility testing was performed by diffusion method according to CLSI standards 2011 for seven antibiotics. The results of molecular testing showed that all the studied strains produced an amplicon of 1000 bp with ompP2 primers indicating that all strains were H. influenzae. For six strains, the PCR amplicon obtained with bexA specific primers, proving that the strains were capsulated. The results of phenotypic testing showed that four strains were ampicillin nonsusceptible and (beta-lactamase-positive. The virulence potential of H. influenzae clinical strains was investigated by phenotypic methods, including the assessment of the soluble virulence factors on specific media containing the biochemical substratum for the investigated enzymatic factor, as well as the adherence and invasion capacity to HeLa cells monolayer using Cravioto modified method. The studied strains exhibited mainly a diffuse adherence pattern and different adherence indexes. Interestingly, two strains isolated from the same pacient (blood and CSF) showed a different degree of invasiveness, the strain isolated from blood being 20 times more invasive than the one isolated from CSF.


Usein C.-R.,Cantacuzino National Institute of Research Development for Microbiology and Immunology | Usein C.-R.,Carol Davila University of Medicine and Pharmacy | Militaru M.,Cantacuzino National Institute of Research Development for Microbiology and Immunology | Cristea V.,Synevo Laboratories | Straut M.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Memorias do Instituto Oswaldo Cruz | Year: 2014

For the first time, we used multilocus sequence typing (MLST) to understand how Romanian group B streptococcus (GBS) strains fit into the global GBS population structure. Colonising isolates recovered from adult human females were tested for antibiotic resistance, were molecularly serotyped based on the capsular polysaccharide synthesis (cps) gene cluster and further characterised using a set of molecular markers (surface protein genes, pilus-encoded islands and mobile genetic elements inserted in the scpB-lmb intergenic region). Pulsed-field gel electrophoresis was used to complement the MLST clonal distribution pattern of selected strains. Among the 55 strains assigned to six cps types (Ia, Ib, II-V), 18 sequence types (STs) were identified by MLST. Five STs represented new entries to the MLST database. The prevalent STs were ST-1, ST-17, ST-19 and ST-28. Twenty molecular marker profiles were identified. The most common profiles (rib+GBSi1+PI-1, rib+GBSi1+PI-1, PI-2b and alp2/3+PI-1, PI-2a) were associated with the cps III/ST-17 and cps V/ST-1 strains. A cluster of fluoroquinolone-resistant strains was detected among the cps V/ST-19 members; these strains shared alp1 and IS1548 and carried PI-1, PI-2a or both. Our results support the usefulness of implementing an integrated genotyping system at the reference laboratory level to obtain the reliable data required to make comparisons between countries.


Iancu A.D.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2012

Type 1 diabetes mellitus (T1DM), one of the most prevalent chronic diseases is characterized by the progressive destruction of pancreatic beta cells, leading to insulin deficiency and hyperglycemia. Studies performed on diabetic subjects with prolonged hyperglycemia showed the oxidative stress occurrence followed by molecular, cellular and tissue damage. Currently, reducing the oxidative stress represents a therapeutic target, in order to reduce its complications in diabetic patients. An adequate experimental model of type 1 diabetes represents a prerequisite in oxidative stress study, therefore, we assessed oxidative status in polymorphonuclear cells (PMNs) and peritoneal macrophages using a double transgenic (dTg) mouse model of type 1 diabetes. Our results revealed the increased production of reactive oxygen species (superoxide anion and H2O2) and nitrogen (nitric oxide) species in diabetic mice leading to the idea of oxidative stress model for the study of its complications in type 1 diabetes.


Iancu A.D.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2012

Type 1 diabetes is an autoimmune disease affecting a higher and higher number of persons; for this reason, the study of diabetes, and its complications, has shown a major interest. In order to highlight the modifications appeared in this disease, it is essential to use a suitable model. In "Cantacuzino" NIRDMI there is a double transgenic murine model which develops a fulminating form of type 1 diabetes. Previous studies indicate the usefulness of this diabetic murine model in order to study neuropathy. Capsaicin treatment is one method to reduce neuropathic pain. This study was based on the assumption that intraperitoneal administration of a low dose of capsaicin, on a short period of time, can decrease pain sensations generation and transmission. If from the neurological point of view, capsaicin effects are known, its effects on the immune system are not clear yet. Therefore, in this study we have investigated capsaicin effects on oxygen and nitrogen free radicals generation by phagocytic immune cells, in lymphocyte populations, and also capsaicin effects on plasmatic protein oxidation. Our results point to minor modifications in oxygen reactive species production, simultaneous with a significantly decrease in nitric oxide generation, without affecting lymphocyte populations. Therefore, capsaicin short term administration can be used to reduce pain sensations, without the impairment of immune parameters.


Pana M.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2011

Animal models of infection and protection on the topic of the Streptococcus pneumoniae (S. pneumoniae) have encountered many difficulties generated by low immunogenicity, a characteristic of polysaccharide capsular bacteria and difference of virulence between serotypes and strains. We have explored the immune response after immunization with heat inactivated S. pneumoniae serotype 1, 3 and 6B in C57BL/6 mice by IgM and IgG detection, and by splenocyte in vitro 5-ethynyl-2'-deoxyuridine (EdU) incorporation after antigen specific stimulation, as a proposed method of cellular immune response evaluation. Antibody titer persistence after immunization was not lengthy while antigen specific proliferation response detected by EdU assay was remnant. Intraperitoneal (i.p.) challenge with serotype 6B S. pneumoniae proved that antibody titers and the detected specific cellular immune response do not cover seroprotective necessity and do not confer improved immunologic memory in comparison to non-immunized mice, which show natural resistance.


Codita I.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2010

Contaminated surfaces are possible vehicles in infection transmission. It is known that both Copper (Cu) and Silver (Ag) efficiently inactivate microbes by direct contact. Aiming at using these metals for benefitting from their antimicrobial effect, but to avoid subsequent toxic effects, we evaluated the antimicrobial activity of nanometric thin Silver and Copper films covering less expensive materials. Using a modified version of the Japan Industrial Standard JIS Z 2801:2000, we demonstrated the antimicrobial activity of the surfaces covered with metal ions nanofilms on microorganisms possibly involved in nosocomial infections and on Bacillus anthracis, bacteria with possible implication in bioterrorist attacks. Copper covered surfaces proved to have better antimicrobial activity than Silver surfaces. Silver covered surfaces showed better activity on Gram negative bacteria than on Gram positive cocci. Going deeper with studies on antimicrobial effects using new methods with better direct and/or functional discriminatory capacity is needed in order to provide additional information on the mechanisms of Silver and Copper nanofilms antimicrobial activity.


Ulea I.,Cantacuzino National Institute of Research Development for Microbiology and Immunology
Roumanian archives of microbiology and immunology | Year: 2013

Thin layer chromatography (TLC) could easily and rapidly evidentiate the qualitative differences between glycolipids (GLs). Different immunomagnetically purified mycobacterial GLs have been compared using TLC, in order to choose the most appropriate antigens to be utilized in ELISA. The GLs were purified from environmental mycobacteria (EM) (M. avium-intracellulare, M. kansasii, M. xenopi, M. scrofulaceum and M. gordonae) and from M. tuberculosis H37Rv. BioMag Amine and BioMag Carboxyl terminated superparamagnetic microparticles were utilized in the magnetic separation of glycolipids from mycobacterial species. TLC of GLs before and after magnetic purification, corroborated with ELISA results, shows that COOH-terminated particles allow a better purification for M. kansasii, M. xenopi and M. scrofulaceum, while NH2-terminated particles act better on MAI and M. gordonae GLs. The use of GL purified antigens in ELISA could fulfill the criteria of high levels of both sensitivity and specificity of serologic assays in EM diagnosis.

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