National Institute of Research and Physico chemical Analyses

Ariana, Tunisia

National Institute of Research and Physico chemical Analyses

Ariana, Tunisia
SEARCH FILTERS
Time filter
Source Type

Rhimi W.,University of Carthage | Immediato D.,University of Bari | Boulila A.,National Institute of Research and Physico chemical Analyses | Cafarchia C.,University of Bari
Molecules | Year: 2017

The small amount of data regarding the antifungal activity of Dittrichia viscosa (L.) Greuter against dermatophytes, Malassezia spp. and Aspergillus spp., associated with the few comparative studies on the antimicrobial activity of methanolic, ethanolic, and butanolic extracts underpins the study herein presented. The total condensed tannin (TCT), phenol (TPC), flavonoid (TFC), and caffeoylquinic acid (CQC) content of methanol, butanol, and ethanol (80% and 100%) extracts of D. viscosa were assessed and their bactericidal and fungicidal activities were evaluated. The antibacterial, anti-Candida and anti-Malassezia activities were evaluated by using the disk diffusion method, whereas the anti-Microsporum canis and anti-Aspergillus fumigatus activities were assessed by studying the toxicity effect of the extracts on vegetative growth, sporulation and germination. The methanolic extract contained the highest TPC and CQC content. It contains several phytochemicals mainly caffeoylquinic acid derivatives as determined by liquid chromatography with photodiode array and electrospray ionisation mass spectrometric detection (LC/PDA/ESI-MS) analysis. All extracts showed an excellent inhibitory effect against bacteria and Candida spp., whereas methanolic extract exhibited the highest antifungal activities against Malassezia spp., M. canis and A. fumigatus strains. The results clearly showed that all extracts, in particular the methanolic extract, might be excellent antimicrobial drugs for treating infections that are life threatening (i.e., Malassezia) or infections that require mandatory treatments (i.e., M. canis or A. fumigatus). © 2017 by the authors. Licensee MDPI.


Ben Salem I.,National Center for Nuclear Science and Technology | Mezni M.,National Institute of Research and Physicochemical Analysis | Boulila A.,National Institute of Research and Physico chemical Analyses | Hamdi M.,Tunisian National Institute of Applied Sciences and Technology | Saidi M.,National Center for Nuclear Science and Technology
Current Microbiology | Year: 2016

The decomposition of penicillin G and erythromycin antibiotics at concentration of 0.2 mg ml−1 by gamma irradiation at 50 kGy followed by biological treatment with Cupriavidus metallidurans CH34 was evaluated. Degradation of penicillin G and erythromycin was analyzed using nuclear magnetic resonance analysis (NMR), fourier transform infrared spectroscopy (FTIR), and chemical oxygen demand (COD). The exposure to the absorbed dose of 50 kGy caused degradation of penicillin G and erythromycin in the aqueous solution. The complete disappearance of NMR and FTIR peaks following irradiation confirmed the breakage of the β-lactam ring in penicillin G, and the decarboxylation and cleavage of the thiazolidine ring and for erythromycin, the complete destruction of the three aromatic rings. Irradiation alone removed 52.8 and 65.5 % of penicillin G and erythromycin, respectively. Further reduction to 12.6 and 14 % of the original penicillin G and erythromycin COD, respectively, was achieved using treatment of the irradiation products with C. metallidurans. © 2016, Springer Science+Business Media New York.


Rokbeni N.,National Institute of Research and Physico Chemical Analyses | M'Rabet Y.,National Institute of Research and Physico Chemical Analyses | Dziri S.,National Institute of Research and Physico Chemical Analyses | Chaabane H.,National Institute of Research and Physico Chemical Analyses | And 3 more authors.
Chemistry and Biodiversity | Year: 2013

The essential oils of Daucus carota L. (Apiaceae) seeds sampled from ten wild populations spread over northern Tunisia were characterized by GC-FID and GC/MS analyses. In total, 36 compounds were identified in the D. carota seed essential oils, with a predominance of sesquiterpene hydrocarbons in most samples (22.63-89.93% of the total oil composition). The main volatile compounds identified were β-bisabolene (mean content of 39.33%), sabinene (8.53%), geranyl acetate (7.12%), and elemicin (6.26%). The volatile composition varied significantly across the populations, even for oils of populations harvested in similar areas. The chemometric principal component analysis and the hierarchical clustering identified four groups, each corresponding to a composition-specific chemotype. The in vitro antimicrobial activity of the isolated essential oils was preliminarily evaluated, using the disk-diffusion method, against one Gram-positive (Staphylococcus aureus) and two Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), as well as against a pathogenic yeast (Candida albicans). All tested essential oils exhibited interesting antibacterial and antifungal activities against the assayed microorganisms. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.


PubMed | Tunisian National Institute of Applied Sciences and Technology, National Center for Nuclear Science and Technology, National Institute of Research and Physicochemical Analysis and National Institute of Research and Physico chemical Analyses
Type: Journal Article | Journal: Current microbiology | Year: 2016

The decomposition of penicillin G and erythromycin antibiotics at concentration of 0.2mgml(-1) by gamma irradiation at 50kGy followed by biological treatment with Cupriavidus metallidurans CH34 was evaluated. Degradation of penicillin G and erythromycin was analyzed using nuclear magnetic resonance analysis (NMR), fourier transform infrared spectroscopy (FTIR), and chemical oxygen demand (COD). The exposure to the absorbed dose of 50kGy caused degradation of penicillin G and erythromycin in the aqueous solution. The complete disappearance of NMR and FTIR peaks following irradiation confirmed the breakage of the -lactam ring in penicillin G, and the decarboxylation and cleavage of the thiazolidine ring and for erythromycin, the complete destruction of the three aromatic rings. Irradiation alone removed 52.8 and 65.5% of penicillin G and erythromycin, respectively. Further reduction to 12.6 and 14% of the original penicillin G and erythromycin COD, respectively, was achieved using treatment of the irradiation products with C.metallidurans.


Boulila A.,National Institute of Research and Physico chemical Analyses | Sanaa A.,Tunisian National Institute of Applied Sciences and Technology | Salem I.B.,Research Unit | Rokbeni N.,National Institute of Research and Physico chemical Analyses | And 3 more authors.
Industrial Crops and Products | Year: 2015

The phenolic profile of six wild growing Marrubium vulgare L. (Lamiaceae) collected from different bioclimatic zones was investigated. Ethanol, ethyl acetate and residual water fractions were investigated for their antioxidant activity using DPPH assay. Ethyle acetate (EtOAc) fraction showed the highest antioxidant capacity with IC50 values ranging from 68.98 to 118.15μg/mL.Eight phenolic acids and five flavonoids were detected in the ethyle acetate fraction and quantified by high performance liquid chromatography (HPLC) coupled to a diode array detector (DAD), among them the concentration of quercetin-3-. d-O-galactoside (5.84-14.46. mg/g DW) was the highest. Metabolic profile, consisting of glycosylated flavonoids and phenolic acids, was very regular with only quantitative differences and absences limited to minor components. A remarkably significant quantitative variation for the major components was noted. On the basis of chemical composition, principal component analysis and cluster analysis, M. vulgare was classified into two groups. Population grouping was not concordant with bioclimatic appurtenance.These results indicate that M. vulgare can be used in dietary applications with a potential to reduce oxidative stress. The pronounced chemical diversity between populations is discussed to possibly be linked to the variation of soil properties and microclimate conditions. © 2015 Elsevier B.V.


Younsi F.,University of Carthage | Trimech R.,University of Carthage | Boulila A.,National Institute of Research and Physico Chemical Analyses | Ezzine O.,National Water Research Institute | And 3 more authors.
International Journal of Food Properties | Year: 2016

Total phenols, flavonoids, flavonols, and flavanols of the methanolic extract of the aerial part of Artemisia herba-alba were determined. The extract was analyzed by liquid chromatography with photodiode array coupled with electrospray ionisation mass spectrometry and allowed to identify of 10 phenolic compounds. Apigenin-6-C-glycosyl flavonoids and caffeoylquinic acids were identified. Chlorogenic acid and 1,4 dicaffeoylquinic acid being the major constituents. The essential oil obtained by hydrodistillation was analyzed by gas chromatography-mass spectrometry. Twenty-three compounds, representing 97.8% of the total oil, were identified. The most abundant components were β-thujone (41.9%), α-thujone (18.4%), and camphor (13.2%). Methanolic extract and essential oil exhibited a considerable antioxidant activity as evaluated by 2,2-diphenyl-pycrilhydrazil hydrate scavenging activity, reducing power, β-carotene bleaching test, and chelating ability. The methanolic extract was found to be more efficient, while the essential oil exhibited the highest acetylcholinesterase inhibitory activity. Analysis of the antibacterial activity showed that A. herba-alba methanolic extract and essential oil are efficient against gram positive and gram negative bacteria. Copyright © Taylor & Francis Group, LLC.


Abdennacer B.,National Institute of Research and Physico chemical Analyses | Karim M.,National Institute of Research and Physico chemical Analyses | Yassine M.,National Institute of Research and Physico chemical Analyses | Nesrine R.,National Institute of Research and Physico chemical Analyses | And 2 more authors.
Food Chemistry | Year: 2015

A comparative analysis of methanol extracts from fruit and leaves of Lycium intricatum Boiss., a Solanaceous shrubbery with the potential to become a high-value crop, was performed by means of liquid chromatography with photodiode array and electrospray ionisation mass spectrometric detection (LC/PDA/ESI-MS). The total phenolic (TPC), anthocyanin (TAC) and flavonoid (TFC) contents as well as the antioxidant capacity measured by four complementary methods were performed for each sample. The results showed the tested extracts to be rich sources of phenolics; in leaves polyphenols and flavonoids dominate, while in fruit anthocyanins dominate. Nineteen phenolic compounds were detected and fifteen were identified or tentatively characterised based on Photodiode-array ultraviolet visible (PDA) UV-Vis spectra, ESI-MS spectrometric data and spiking experiments with authentic standards. Rutin and chlorogenic acid are the major constituents of the leaves and fruit, respectively. Results obtained in this study have revealed that leaves exhibit better performance in all antioxidant assays. From these results it has been shown that extracts of L. intricatum have great potential as a source of phenolics for natural health products. © 2014 Elsevier Ltd. All rights reserved.


PubMed | National Institute of Research and Physico Chemical Analyses
Type: Journal Article | Journal: Chemistry & biodiversity | Year: 2013

The essential oils of Daucus carota L. (Apiaceae) seeds sampled from ten wild populations spread over northern Tunisia were characterized by GC-FID and GC/MS analyses. In total, 36 compounds were identified in the D. carota seed essential oils, with a predominance of sesquiterpene hydrocarbons in most samples (22.63-89.93% of the total oil composition). The main volatile compounds identified were -bisabolene (mean content of 39.33%), sabinene (8.53%), geranyl acetate (7.12%), and elemicin (6.26%). The volatile composition varied significantly across the populations, even for oils of populations harvested in similar areas. The chemometric principal component analysis and the hierarchical clustering identified four groups, each corresponding to a composition-specific chemotype. The in vitro antimicrobial activity of the isolated essential oils was preliminarily evaluated, using the disk-diffusion method, against one Gram-positive (Staphylococcus aureus) and two Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), as well as against a pathogenic yeast (Candida albicans). All tested essential oils exhibited interesting antibacterial and antifungal activities against the assayed microorganisms.

Loading National Institute of Research and Physico chemical Analyses collaborators
Loading National Institute of Research and Physico chemical Analyses collaborators