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Liliana B.C.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Angela M.,Transilvania University of Brasov | Nicoleta C.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Florentina D.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Andreea N.,National Institute of Research and Development for Potato and Sugar Beet Brasov
Romanian Biotechnological Letters | Year: 2011

The potato virus Y causes loss of yield and quality of tubers. Antioxidants such as rosmarinic acid (present in oils extracted from Rosmarinus officinalis plants), hydrogen peroxide and ascorbic acid are implicated in signaling against stress. The effects of these chemicals on photosynthetic pigments content and minituber yield were evaluated in plants testing positive after virus mechanical infection. Without chemical treatment, positive plants showed significant reductions in leaf pigments content and tuber weights compared to uninfected controls. Hydrogen peroxide, ascorbic acid and oil treatments of positive plants significantly reduced the number of minitubers, enhancing their weights, while leaf pigment content also increased. This research demonstrated the potential benefits of treatments with oils extracted from Rosmarinus officinalis plants and hydrogen peroxide or ascorbic acid in enhancing the yield and quality of tubers. © 2011 University of Bucharest. Source


Chiru N.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Nistor A.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Karacsonyi D.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Chiru R.N.,University of Bucharest
Annals of the Romanian Society for Cell Biology | Year: 2010

A technique for production of in vitro microtubers >10 mm was developed for some potato varieties (Solanum tuberosum L.). Uninodal cuttings from potato microplants propagated in vitro were utilized as initial ex-plants. These were placed in culture vessel containing medium with nutritional solutions specific for each phase of tuberisation process (growing, inducing and tuberisation). The plant material used, came from three Romanian potato cultivars (Roclas, Christian and Tampa), using into three variants of working: 15 and 25 uninodal cuttings/ vessel and five plantlets / vessel placed horizontally. For each stage of development (growth, inducing), cultures were kept under controlled conditions of light and temperature (16 hours of light/8 hours of dark, t° 20 ± 1°C/day and 18 ± 1°C/night). The cultures, over which to induce microtuberization, was added tuberization liquid medium, were transferred to the climate room at darkness, at a temperature of about 14 to 16°C. After roughly 6-7 weeks were obtained microtubers, improved on both in terms of number and size (> 10 mm) and also of weight (>950g). Source


Badarau C.L.,Transilvania University of Brasov | Marculescu A.,Transilvania University of Brasov | Chiru N.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Chiru S.C.,National Institute of Research and Development for Potato and Sugar Beet Brasov
Romanian Agricultural Research | Year: 2012

Antioxidants such as rosmarinic acid, chlorogenic acid and poliphenols, present in essential oils extracted from Lamiaceae family plants, as well as many other compounds like hydrogen peroxide and ascorbic acid are implicated in the stress signalling process. The effects of treatments with essential oils from Rosmarinus officinalis (for potato) and from Thymus serpyllum or Lavandula officinalis (for tobacco) on pigments content, minituber yield (in potato) and on antiviral activity (in tobacco) were evaluated after virus mechanical inoculation. The treatments of positive potato plants significantly reduced the number of minitubers, increasing their weight, while leaf pigment content also increased. Concerning the antiviral effect of the Thymus serpyllum and Lavandula officinalis oils, all the treated tobacco plants presented after PVY infection values of absorbance at 405nm significantly lower than the untreated and inoculated controls. This research demonstrates potential benefits of Rosmarinus officinalis oils in enhancing the potato yield and quality of tubers and of Thymus serpyllum or Lavandula officinalis oils on the immunization of Nicotiana tabacum. Source


Nistor A.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Karacsonyi D.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Chiru N.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Campeanu G.,University of Bucharest | Atanasiu N.,University of Bucharest
Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii | Year: 2010

Potato seed production represents a major interest of scientific research for this culture. Hydroponic system offers possibilities of production minitubers, with a superior sanitary quality. During the year 2009, the varieties Ostara, Christian, Roclas were experimented at National Institute of Research and Development for Potato and Sugar Beet Brasov on this system, comparative with classical technology on culture in greenhouse, using as biological material minitubers and plantlets. Analyzing the behavior of this varieties on different sublayers culture (expanded clay granules, perlite, soil), resulted a difference between experimented varieties and sublayers used. Christian and Ostara varieties have been registered the best results, concerning the minitubers number/plant, with values between 6.5 and 7.66 minitubers/plant. Using of hydroponic system had a positive influence on the minitubers number/plant, comparative with classical method of culture in greenhouse, on soil. This had been reflected by production of a big number of minitubers/plant, on the sublayer with expanded clay (8.25 minitubers/plant), followed by the sublayer with perlit (6.41 minitubers/plant). From view point of interaction between varieties and culture sublayer, concerning minitubers number/plant, the highest values were find at Ostara varieties on sublayer with expanded clay granules (10.5 minitubers/plant), followed by Christian on sublayer with perlit (9.5 minitubers/plant. Regarding the minitubers distribution on calibration class, the highest frequency of minitubers production is on calibration class 15-25 mm (for 9 cases), followed by calibration class <15 mm (8 cases). At calibration class 35-45 mm, production rate is very small (discovering only at Ostara variety in 3 cases). The influence of sublayer culture on size order of minitubers is reflecting by appearance of all calibration classes for the 3 sublayers, but with preponderance, the highest values are finding al 15-25 mm calibration class with a sum up value of 7.4 minitubers and 7.2 minitubers for calibration class <15 mm. Regarding the total weight of minitubers obtained/ plant, analyzed in function of sublayer, it may be note the positive influence of sublayers with expanded clay granules and perlite, used on hydroponic system, towards the soil, used in classical technology, the values being close between the two kinds of industrial sublayer (values with 34.27 and 33.27 g). © 2010 Vasile Goldis University Press. Source


Nistor A.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Campeanu G.,University of Agronomical Sciences and Veterinary Medicine | Atanasiu N.,University of Agronomical Sciences and Veterinary Medicine | Chiru N.,National Institute of Research and Development for Potato and Sugar Beet Brasov | Karacsonyi D.,National Institute of Research and Development for Potato and Sugar Beet Brasov
Romanian Biotechnological Letters | Year: 2010

"In vitro" microtuberization represents the transitory phase from "in vitro" multiplication of a healthy material to on field growing. Microtuber production is an efficient method for obtaining a healthy material, through which the process of potato production is reduced with 3-4 years. In the same time the microtubers are important because they can be produced in any period of the year, they are easy to be transported and stored.. Microtubers of semi-early Romanian varieties (Christian and Roclas) and of early Dutch variety (Ostara) were obtained from potato micro-cutting cultures on Murashige-Skoog medium enriched with Cumarin and Kinetin. The sucrose was the most important stimulus for inducing the microtubers. Microtuber inducing and growing was achieved in cultures maintained in darkness, at 18-20°C for 8-10 weeks. © 2010 University of Bucharest. Source

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