National Institute of Research and Development for Potato and Sugar Beet

Braşov, Romania

National Institute of Research and Development for Potato and Sugar Beet

Braşov, Romania

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Cojocaru N.,National Institute of Research and Development for Potato and Sugar Beet | Badarau C.L.,National Institute of Research and Development for Potato and Sugar Beet | Badarau C.L.,Transilvania University of Brasov | Stroe F.,National Institute of Research and Development for Potato and Sugar Beet
Analele Universitatii din Oradea, Fascicula Biologie | Year: 2016

The objective of this research was to study if the frozen material could be used to obtain a PVY suspension with a proper concentration for antisera preparation. The virus extraction and purification was achieved from frozen leaves and fresh tobacco leaves (cv. 'Samsun' and 'White Burley') mechanically inoculated with a PVYN infected material (cv. 'Record' secondary infection with this pathogen). The experiment followed a schema used in other virology laboratories with some modifications particulary quantitative. Results of this preliminary studies indicated that frozen PVYN infected material is not advisible for the preparation of a virus suspension solution to be used for antisera production.


Damsa F.,Polytechnic University of Bucharest | Damsa F.,National Institute of Research and Development for Potato and Sugar Beet | Woinaroschy A.,Polytechnic University of Bucharest | Olteanu G.,National Institute of Research and Development for Potato and Sugar Beet | And 2 more authors.
Revista de Chimie | Year: 2016

The recent interest on phenolic bioactive compounds has caused an increased attention for optimizing the techniques used in bioactive phytochemicals extraction from different natural sources. In order to determine the best extraction conditions using direct sonication were varied amplitude (20, 50 and 80%) ant sonication time (5, 15 and 25 min). To optimize the extraction process was performed mathematical modeling (full factorial experiments 22) using SigmaXL software. The total anthocyanins content were determined spectrophotometrically by the pH differential method and the total flavonoids content were determine colorimetric by AlCl3 method.


Damsa F.,Polytechnic University of Bucharest | Damsa F.,National Institute of Research and Development for Potato and Sugar Beet | Woinaroschy A.,Polytechnic University of Bucharest | Olteanu G.,National Institute of Research and Development for Potato and Sugar Beet
Revista de Chimie | Year: 2015

In order to determine the total flavonoid and total anthocyanin contents were used crude extracts from purple potato (Blue-violet ofGalanesti - Romania variety)). The amount of total flavonoid (TFC) was analysed using aluminium chloride colorimetric assay and the amount of total anthocyanin (TAC) was analysed using pH differential assay. For the flavonoids and anthocynins extraction were used different solvents (1% acidified methanol, 1% acidified ethanol and 1% acidified deionized water) and different contact time between solvent and sample (30,45,60 min). Methanolic extract showed highest amount also for TAC (140.703 mg/ l00gFW) and for TFC (105.305 mg/l00gFW). Ethanolic extract showed lowest amount also for TAC (66.600 mg/l00g FW) and for TFC (25.37 mg/l00g FW).


Karacsonyi D.E.,National Institute of Research and Development for Potato and Sugar Beet | Chiru N.,National Institute of Research and Development for Potato and Sugar Beet | Nistor A.,National Institute of Research and Development for Potato and Sugar Beet
Romanian Biotechnological Letters | Year: 2011

Since the discovery of PCR (polymerase chain reaction) and development of several PCR based methods, the genetic analysis became a useful tool for plant selection and cultivar accurate identification. The aim of this study was to identify the in vitro somaclonal variation in plants regenerated from callus, using simple sequence repeats (SSR markers). © 2011 University of Bucharest.


Nistor A.,National Institute of Research and Development for Potato and Sugar Beet | Chiru N.,National Institute of Research and Development for Potato and Sugar Beet | Cioloca M.,National Institute of Research and Development for Potato and Sugar Beet | Badarau C.,National Institute of Research and Development for Potato and Sugar Beet
Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii | Year: 2015

This paper had as principal objective identification of potato varieties with tolerance to water stress by using “in vitro” tissue culture and mannitol, in different concentration, as an agent to induce water stress simulation. Mannitol added to the culture nutritive medium had five levels (0.36, 0.73, 1.11, 1.46,1.92%). The starting point was represented by plantlets culture collection, belonging to Romanian varieties: Rustic, Tampa, Ruxandra, Robusta, Gared. Plantlets were multiplied between two internodes to obtain microcuttings (in sterile condition), which were inoculated on medium established by Murashighe & Skoog (1962), enriched with vitamins to which is added sucrose, agar, growth regulators (for control media). There were other variants of media, to which was added at each mannitol concentrations. After 4 weeks was made the determination of values for different morphological parameters (plantlets height, number of leaves/plantlet and fresh weight of plantlets) - which were statistically analyzed by Duncan test. Water stress affected plantlets length, number of leaves and plantlets fresh weight. The varieties showed different responses. Plantlets height decreased dramatically by exposure to high concentrations of mannitol. This is due to a possible mechanism of water stress intolerance. “In vitro” simulation of water stress for Romanian potato varieties, under the influence of a high osmotic potential, may provide an efficient system of differentiation for varieties tolerant to drought, under field conditions. © 2015 Vasile Goldis University Press.

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