National Institute of Research and Development for Food Bioresources

Bucharest, Romania

National Institute of Research and Development for Food Bioresources

Bucharest, Romania
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Palade L.M.,University of Bucharest | Palade L.M.,National Institute for Research and Development in Animal Biology and Nutrition | Duta D.,National Institute of Research and Development for Food Bioresources | Popescu C.,University of Bucharest | And 2 more authors.
Romanian Biotechnological Letters | Year: 2016

The primary flavours of musts and wines are generally constituted by the terpenes and terpenoids, norisoprenoids, benzol derivatives, aliphatic and glycosidic substances, carotene substances. The objective of this study was to differentiate among three must samples (Merlot, Cabernet Sauvignon and Feteasca Neagra) based on their volatile profile. The grape samples were obtained from Murfatlar vineyard (Romania). The wines produced from these grapes are labelled "Protected Denomination of Origin" (PDO) and the finding of certain markers for their authenticity should be of great interest for the wine producers. The headspace analysis (GC-MS) was employed along with the a- Prometheus multi-sensor system used for the food quality control (electronic nose), which provides pattern recognition of the flavouring compounds present in the three must samples. The study reveals differences in the volatile chemical compounds profiles of the analysed varieties. This study may be used as a starting point for developing GC-MS and electronic nose valuable methods for the authentication of the grape variety. © 2016 University of Bucharest.

Jurcoane A.P.,UASVM Bucharest | Duta D.,National Institute of Research and Development for Food Bioresources | Niculita P.,UASVM Bucharest
Romanian Biotechnological Letters | Year: 2012

This paper is a product of a doctoral thesis on developing oat-based functional products in correlation with consumer perception of health risks, funded by POSDRU project 107/1.5/S/76888. The analysis of the recent literature revealed a massive interest in oat-based functional products and that technological improvements were made to develop more such products. Oat proved to be a valuable ingredient that could improve considerably the nutritious value of cereal products like bread, pasta, noodles or cakes and, more specifically, gluten-free products. The main technological improvements investigated were the utilization of process optimization in terms of water and gluten addition, use of high hydrostatic pressure, enzyme treatment, sourdough addition, endogenous enzyme inactivation and, specifically for gluten-free products, replacement of α-gliadins with oat avenins. Recent studies also showed extrusion to be an advantageous method to produce oat-based products. New health benefits of oats with health claim potential were identified, like antioxidant capacity, reduction of atherogenesis, and hypoglycaemic and inhibitory effects on intestinal dissaccharidases. Moreover, the literature highlighted the importance of consumer acceptance and appeal in order to include oat-based functional food in their daily diets so to reach the necessary intake levels to get the currently claimed health effects. © 2012 University of Bucharest.

NegoitA M.,National Institute of Research and Development for Food Bioresources | Culetu A.,National Institute of Research and Development for Food Bioresources
Journal of the Brazilian Chemical Society | Year: 2016

A gas chromatography tandem mass spectrometry has been developed and validated for the separation, detection, identification and quantification of acrylamide in bread, biscuits and similar products. The method showed good precision with values lower than 6%. A good sensitivity was achieved for bread with 2.41 and 7.23 μg kg-1 limit of detection (LOD) and limit of quantification (LOQ), respectively, while for biscuits, LOD and LOQ were 4.63 and 13.89 μg kg-1, respectively. Accuracy obtained through the bias of 2 certified reference materials ("crisp bread - ERM®- BD272" and "rusk - ERM®-BD274") gave a value below 1.68-2.52%. The method was applied by analyzing 49 types of bread, biscuits and other similar products. The results showed different levels of acrylamide in bread (values ranged between 7.6 and 165.6 μg kg-1), biscuits (between LOD and 2405.0 μg kg-1), sandwich biscuits with cream (112.6-570.4 μg kg-1), biscuits for infants and young children (between LOD and 801.7 μg kg-1), gingerbread (349.5-955.5 μg kg-1) and crackers (347.8-366.1 μg kg-1). ©2016 Sociedade Brasileira de Química.

Croitoru C.,Bioterra University | Croitoru C.,National Institute of Research and Development for Food Bioresources
Journal of Food, Agriculture and Environment | Year: 2013

The new procedure enables the rapid and efficient demetallization of all red wines without affecting their chromatic characteristics in such manner that it also allows maintaining a small remanent content (under 4-5 mg/L Fe, under 1 mg/L Cu). This is a consequence of the total HCN concentration monitorization that always corresponds to a difference of titration with a solution of AgNO3 (N/1000) which is inferior to a volume of 0.5 ml related to the titration in the control sample, not treated with K4[Fe(CN)6]. The novelty consists in the localisation of the critical zone in which Fe2+ and [Fe(CN)6]4- ions coexist around a graphical curve that represents the total Fe content variation as a function of the K4[Fe(CN)6] doses that were administrated. Concerning the demetallization of type A food spirits (Fe < 50 mg/L, Cu > 6 mg/L), it is assured a fast flocculation of metallic ferrocyanide colloides formed before adding the fining agents, because metallic ions are not masked as complex that could keep them sequestered. The demetallization of type B food spirits (Fe< 50 mg/L, Cu < 6 mg/L) by a simple enrichment with iron salts lead to the forming of ferric ferrocyanide that acted as a real ion exchanger for copper cations (Cu+and Cu2+) and, probably, for other heavy metal cations. The increase of the alcoholic concentration, maintaining constant the total Fe and Cu content, highlighted a rise of K4[Fe(CN)6] dose necessary for demetallization. When doubled the alcoholic concentration and variated pH, at the constant Fe and Cu contents, at superior oxidation states administrated in spirits, K4[Fe(CN)6] dose varied in an insignificant mode, but assured an advanced demetallization.

Cimponeriu D.,University of Bucharest | Ion D.A.,Carol Davila University of Medicine and Pharmacy | Spandole S.,University of Bucharest | Apostol P.,University of Bucharest | And 13 more authors.
Romanian Biotechnological Letters | Year: 2013

The complex genetic susceptibility for breast cancer includes highly penetrating and lowpenetrating genetic markers. eNOS (7q36), ACE (17q23) and AGTR1 (3q24) are candidate loci for breast cancer. The aim of this study was to test the potential association of eNOS 4ab, ACE ID, AGTR1 A1166T polymorphisms and Torque teno virus (TTV) infections with sporadic breast cancer in Romania. For this case-control study were selected women with sporadic breast cancer (n=100) and healthy women (n=100). The eNOS 4ab, ACE ID, AGTR1 A1166T polymorphisms and presence of TTV DNA were assessed by PCR-based protocols. The polymorphisms' distribution was in accordance with Hardy-Weinberg equilibrium. No significant association with breast cancer was found for investigated polymorphisms considered individually or in combinations of two or three markers (p>0.05). TTV infections were more frequent in cancer patients than in control (84% vs. 60%, p=0.00016). A preferential distribution of TTV in patients with sporadic breast cancer, especially in those carrying eNOS bb genotype (OR=4.88, 95%CI: 2.01- 11.9, p=0.00025) or ACE I variant (OR=4.07, 95%CI: 1.85- 8.97, p=0.0003), was observed. This study revealed a preferential distribution of TTV infections in women with sporadic breast cancer, carriers of eNOS bb genotype or ACE I variant.©2013 University of Bucharest.

Cimponeriu D.,University of Bucharest | Serafinceanu C.,Nicolae Paulescu National Institute of Diabetes | Apostol P.,University of Bucharest | Toma M.,University of Bucharest | And 8 more authors.
Central European Journal of Biology | Year: 2013

Polymorphisms in IL6, ACE and ATR genes are associated with obesity. Torque Teno virus (TTV) seems to be able to interfere with production of some proinflammatory cytokines associated with obesity and related phenotypes. The aim of this study was to test the potential association between obesity, TTV infection and the IL6 G-174C (rs1800795), ACE I/D (rs4646994), AT1R A1166C (rs5186) polymorphisms. The polymorphisms and the presence of TTV were detected in blood samples from 150 obese and 150 normal-weight, healthy subjects using PCR based methods. IL6-174 CC genotype was more frequent in all obese patients (P=0. 02) and in patients without TTV infections (P=0. 03) than in controls. Obese women had more frequent TTV infections compared with normal-weight women (P=0. 046). Obese subjects, regardless of gender (women P=0. 03, men P=0. 04), and healthy normal-weight men (P<0. 01) carriers of AT1R C allele had higher triglycerides levels compared with non-carriers. The frequency of TTV in the control group (70. 67%) was similar to data reported in other populations. The present study indicated that IL6-174 CC genotype and TTV infections in women could be associated with the common form of obesity. © 2013 Versita Warsaw and Springer-Verlag Wien.

Spandole S.,University of Bucharest | Cimponeriu D.,University of Bucharest | Berca L.M.,National Institute of Research and Development for Food Bioresources | Mihaescu G.,University of Bucharest
Archives of Virology | Year: 2015

Human torque teno viruses (TTVs) are new, emerging infectious agents, recently assigned to the family Anelloviridae. The first representative of the genus, torque teno virus (TTV), was discovered in 1997, followed by torque teno mini virus (TTMV) in 2000, and torque teno midi virus (TTMDV) in 2007. These viruses are characterized by an extremely high prevalence, with relatively uniform distribution worldwide and a high level of genomic heterogeneity, as well as an apparent pan-tropism at the host level. Although these viruses have a very high prevalence in the general population across the globe, neither their interaction with their hosts nor their direct involvement in the etiology of specific diseases are fully understood. Since their discovery, human anelloviruses, and especially TTV, have been suggested to be associated with various diseases, such as hepatitis, respiratory diseases, cancer, hematological and autoimmune disorders, with few arguments for their direct involvement. Recent studies have started to reveal interactions between TTVs and the host’s immune system, leading to new hypotheses for potential pathological mechanisms of these viruses. In this review article, we discuss the most important aspects and current status of human TTVs in order to guide future studies. © 2015, Springer-Verlag Wien.

Culetu A.,University of Applied Sciences and Arts Western Switzerland | Culetu A.,National Institute of Research and Development for Food Bioresources | Fernandez-Gomez B.,Institute of Food Science Research | Ullate M.,Institute of Food Science Research | And 2 more authors.
Food Chemistry | Year: 2016

The antiglycoxidative properties of theanine (TEF) and polyphenols enriched fractions (PEF) prepared from tea dust were tested in a model system composed of bovine serum albumin (BSA) and methylglyoxal (MGO). PEF caused a decrease in available free amino groups of BSA in presence and absence of MGO, suggesting the simultaneous occurrence of glycoxidation reaction and phenols-protein interaction. The presence of PEF and TEF inhibited formation of fluorescent advanced glycation end-products (AGEs). Moreover, theanine (TB) and polyphenol-enriched bread (PB) were formulated. A significant increase in free amino groups was observed in TBs with a dose-response effect, while addition of PEF in bread produced a significant decrease (p < 0.05). PEF efficiently reduced fluorescent AGE formation in breads compared with TEF. The results are in line with the simplified model systems. PEF used as food ingredient allows obtaining a tasty food possessing health promoting properties and lower content of potential harmful compounds (AGEs). © 2015 Elsevier Ltd.

Cristina C.,Titu Maiorescu University | Elena R.,Titu Maiorescu University | Vasilica C.,Titu Maiorescu University | Lavinia-Mariana B.,National Institute of Research and Development For Food Bioresources | And 6 more authors.
Romanian Biotechnological Letters | Year: 2014

T1DM patients without clinical proteinuria (n= 80) and healthy controls (n= 80) were included in this study. The Insulin -23Hph (T/A), Insulin +1127Pst1 (C/T), IGF2 Apa (820 G/A), SELL P213S polymorphisms and NAIP exon 5 deletion were genotyped in all samples. All genotypes were similarly distributed in subjects stratified according to gender. The exon 5 of NAIP gene was present in all samples and this polymorphism was excluded from further analysis. The distribution of Insulin -23Hph, Insulin +1127Pst, IGF2 Apa, SELL P213S genotypes in patients and controls are in agreement with Hardy-Weinberg equilibrium. The Insulin -23Hph AA genotype is significantly associated with T1DM (O.R. AA vs. non-AA = 4.26; 95%CI: 2.06-8.8; p<0.0001). No other significant differences of genotypes, alleles or combined genotypes were detected between investigated markers and diabetes. We found a strong association of polymorphisms from IDDM2 with T1DM in our population. © 2014 University of Bucharest.

Dobre C.,University of Bucharest | Elena T.,University of Bucharest | Ionita L.,National Institute of Research and Development for Food Bioresources | Berca L.M.,National Institute of Research and Development for Food Bioresources
Romanian Biotechnological Letters | Year: 2014

Benincasa Hispida (BHS) is a plant specific to tropical areas, fairly resistant to drought and with various medicinal proprieties, which can be adapted to Romanian pedoclimatic conditions according to the first experiment conducted in the '60s by a group of Romanian researchers. Our purpose was to recreate and extend the experiment in an open field and to analyze the main relations between soil, plants and fruit yield. By conducting this research were emphasized the pedological and climatic necessities for cultivation, the biological comportment of the plant and the main characteristics of the fruit. The main results reveal a good adaptation of this plant, a high productivity and great possibilities of valorization. © 2014 University of Bucharest.

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