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Petric R.C.,Babes - Bolyai University | Petric R.C.,University of Medicine and Pharmacy, Cluj-Napoca | Braicu C.,University of Medicine and Pharmacy, Cluj-Napoca | Braicu C.,Oncology Institute Prof Dr Ion Chiricuta | And 9 more authors.
PLoS ONE | Year: 2015

The use of animal models has facilitated numerous scientific developments, especially when employing "omics" technologies to study the effects of various environmental factors on humans. Our study presents a new bioinformatics pipeline suitable when the generated microarray data from animal models does not contain the necessary human gene name annotation. We conducted single color gene expression microarray on duodenum and spleen tissue obtained from pigs which have been exposed to zearalenone and Escherichia coli contamination, either alone or combined. By performing a combination of file format modifications and data alignments using various online tools as well as a command line environment, we performed the pig to human gene name extrapolation with an average yield of 58.34%, compared to 3.64% when applying more simple methods. In conclusion, while online data analysis portals on their own are of great importance in data management and assessment, our new pipeline provided a more effective approach for a situation which can be frequently encountered by researchers in the "omics" era. © 2015 Petric et al. Source


Taranu I.,National Institute of Research and Development for Biology and Animal Nutrition | Marin D.E.,National Institute of Research and Development for Biology and Animal Nutrition | Burlacu R.,University of Bucharest | Pinton P.,French National Institute for Agricultural Research | And 2 more authors.
Archives of Animal Nutrition | Year: 2010

Mycotoxins are fungal secondary metabolites that elicit a wide spectrum of toxicological effects, including the alteration of normal immune function. In the present study we investigated the independent effect of four mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1), deoxynivalenol (DON) and nivalenol (NIV), on lymphocyte proliferation using human and porcine lymphocytes. Human and porcine peripheral blood mononuclear cells and porcine splenocytes were cultured with increasing concentrations of mycotoxins for 72 hours and labelled in the last 24 hours with [methyl-3H]-thymidine. The results showed that increased concentrations of AFB1, DON and NIV affected the [methyl-3H]-thymidine cellular proliferation following mitogen stimulation in both species and cell types. Lower concentrations of mycotoxins enhanced cellular proliferation, which was more pronounced in human than in porcine cells, while higher concentrations caused a dose-dependent decrease. DON and NIV were the most potent mycotoxin in both species and both cell types. Based on the results of this in vitro study, high correlations were found between proliferation of human and porcine lymphocytes after mycotoxin exposure, especially for DON and NIV. © 2010 Taylor & Francis. Source


Pelmus R.S.,National Research Development Institute of Biology and Animal Nutrition | Pelmus R.S.,National Institute of Research and Development for Biology and Animal Nutrition | Pistol G.C.,National Research Development Institute of Biology and Animal Nutrition | Pistol G.C.,National Institute of Research and Development for Biology and Animal Nutrition | And 5 more authors.
Romanian Biotechnological Letters | Year: 2012

There is an international interest for preservation and improvement of local animal breeds, due to their superior biological traits: rusticity, resistance and adaptability to very different local environment. The local Romanian Teleorman Black Head Tsigai sheep breed fits very well the current economic demands, such as milk production and prolificacy. The purpose of our study was to determine milk quality indices as well as milk protein polymorphism in local sheep breed, using 24 milk samples. The types of different milk proteins were identified by SDS-PAGE. The test day milk yield and chemical composition assays performed during the milking period of studied sheep showed that the yield of milk fat and protein ranged within the quality indices specific to breed (6.56% fat and 5.9% protein), described in literature. Milk samples were further analyzed for milk protein polymorphism. The electrophoretic pattern of milk samples showed the presence of four major caseins variants (αs1-, αs2-β- and k-casein) and two whey proteins (β-lactoglobulin, α-lactalbumin). Our study on percentage analysis of protein fractions of interest revealed that caseins represented 74.16% of the total protein of sheep milk, followed by whey proteins. SDS-PAGE analysis showed that majority of milk samples is characterised by a medium expression level of both caseins and whey proteins (66.67-79.17%) followed by higher level of expression (12.6-29.17%). Further studies (Real Time qPCR) are requisite for certitude of the results on the polymorphic genes of proteins from sheep milk, in order to identify the genetic variants from the locus of each protein. © 2012 University of Bucharest. Source


Taranu I.,National Institute of Research and Development for Biology and Animal Nutrition | Marin D.E.,National Institute of Research and Development for Biology and Animal Nutrition | Manda G.,National Institute of Pathology Victor Babes | Motiu M.,National Institute of Research and Development for Biology and Animal Nutrition | And 4 more authors.
British Journal of Nutrition | Year: 2011

Trichotecenes are mycotoxins produced by Fusarium sp., which may contaminate animal feeds and human food. A feeding trial was conducted to evaluate the effect of a fusarotoxin-contaminated diet, and to explore the counteracting potential of a calcium fructoborate (CFrB) additive on performance, typical health biochemistry parameters and immune response in weaned pigs. A naturally contaminated maize, containing low doses of deoxynivalenol, zearalenone, fumonisins and T-2/HT-2 toxins (1790, 20, 0•6 and 90 parts per billion), was included in a maize-soyabean meal diet, and given ad libitum to eight weaned piglets (two groups: four pigs/group) for a period of 24 d. CFrB was administered to one of the contaminated groups and to another four piglets as a daily supplement, following the manufacturer's recommendation. A decrease in performance was observed in contaminated animals at this concentration of feed toxins, which was ameliorated by the dietary CFrB supplementation. Fusarium toxins also altered the pig immune response by increasing (P < 0•05) the ex vivo peripheral blood mononuclear cell proliferation (111•7 % in comparison with control), the respiratory burst of porcine granulocytes (15•4 % for responsive cells v. 5•1 % for unstimulated cells and 70•95 v. 22•65 % for stimulated cells, respectively), the percentage of peripheral T, CD3+, CD3 +CD4+ and CD3+CD8+ subsets and the synthesis of IL-1β, TNF-α and IL-8 (123•8, 217•1 and 255•1 %, respectively). The diet containing the CFrB additive reduced these exacerbated cellular immune responses induced by Fusarium toxins. However, consumption of CFrB did not counteract the effect of mycotoxins on biochemistry parameters, and increased plasma IgM and IgG of contaminated pigs. © 2011 The Authors. Source


Taranu I.,National Institute of Research and Development for Biology and Animal Nutrition | Gras M.,National Institute of Research and Development for Biology and Animal Nutrition | Pistol G.C.,National Institute of Research and Development for Biology and Animal Nutrition | Motiu M.,National Institute of Research and Development for Biology and Animal Nutrition | And 4 more authors.
PLoS ONE | Year: 2014

Camelina oil-cakes results after the extraction of oil from Camelina sativa plant. In this study, camelina oil-cakes were fed to fattening pigs for 33 days and its effect on performance, plasma biochemical analytes, pro-/anti-inflammatory mediators and antioxidant detoxifying defence in spleen was investigated in comparison with sunflower meal. 24 crossbred TOPIG pigs were randomly assigned to one of two experimental dietary treatments containing either 12% sunflower meal (treatment 1-T1), or 12.0% camelina oil-cakes, rich in polyunsaturated fatty acids ω-3 (ω-3 PUFA) (treatment 2-T2). The results showed no effect of T2 diet (camelina cakes) on feed intake, average weight gain or feed efficiency. Consumption of camelina diet resulted in a significant decrease in plasma glucose concentration (18.47%) with a trend towards also a decrease of plasma cholesterol. In spleen, T2 diet modulated cellular immune response by decreasing the protein and gene expression of pro-inflammatory markers, interleukin 1-beta (IL-1β), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and interleukin (IL-8) and cyclooxigenase 2 (COX-2) in comparison with T1 diet. By contrast, T2 diet increased (P<0.05) in spleen the mRNA expression of antioxidant enzymes, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase 1 (GPx1) by 3.43, 2.47 and 1.83 fold change respectively, inducible nitric oxide synthase (iNOS) (4.60 fold), endothelial nitric oxide synthase (eNOS) (3.23 fold) and the total antioxidant level (9.02%) in plasma. Camelina diet increased also peroxisome-proliferator activated receptor gamma (PPAR-γ) mRNA and decreased that of mitogen-activated protein kinase 14 (p38α MAPK) and nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB). At this level of inclusion (12%) camelina oil-cakes appears to be a potentially alternative feed source for pig which preserves a high content of ω-3 PUFA indicating antioxidant properties by the stimulation of detoxifying enzymes expression and the suppression of spleen pro-inflammatory markers. © 2014 Taranu et al. Source

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