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Chen S.H.,National Institute of Parasitic Disease
PLoS neglected tropical diseases | Year: 2010

BACKGROUND: Pentastomiasis is a rare parasitic infection of humans. Pentastomids are dioecious obligate parasites requiring multiple hosts to complete their lifecycle. Despite their worm-like appearance, they are commonly placed into a separate sub-class of the subphylum Crustacea, phylum Arthropoda. However, their systematic position is not uncontested and historically, they have been considered as a separate phylum. METHODOLOGY/PRINCIPAL FINDINGS: An appraisal of Armillifer agkistrodontis was performed in terms of morphology and genetic identification after its lifecycle had been established in a multi-host model, i.e., mice and rats as intermediate hosts, and snakes (Agkistrodon acutus and Python molurus) as definitive hosts. Different stages of the parasite, including eggs, larvae and adults, were isolated and examined morphologically using light and electron microscopes. Phylogenetic and cluster analysis were also undertaken, focusing on the 18S rRNA and the Cox1 gene. The time for lifecycle completion was about 14 months, including 4 months for the development of eggs to infectious larvae in the intermediate host and 10 months for infectious larvae to mature in the final host. The main morphological difference between A. armillatus and Linguatula serrata is the number of abdominal annuli. Based on the 18S rRNA sequence, the shortest hereditary distance was found between A. agkistrodontis and Raillietiella spp. The highest degree of homology in the Cox 1 nucleic acid sequences and predicted amino acid sequences was found between A. agkistrodontis and A. armillatus. CONCLUSION: This is the first time that a multi-host model of the entire lifecycle of A. agkistrodontis has been established. Morphologic and genetic analyses supported the notion that pentastomids should be placed into the phylum Arthropoda. Source


Shan C.,York University | Zhou X.,National Institute of Parasitic Disease | Zhu H.,York University
Bulletin of Mathematical Biology | Year: 2014

We formulate and analyze a system of ordinary differential equations for the transmission of schistosomiasis japonica on the islets in the Yangtze River, China. The impact of growing islets on the spread of schistosomiasis is investigated by the bifurcation analysis. Using the projection technique developed by Hassard, Kazarinoff and Wan, the normal form of the cusp bifurcation of codimension 2 is derived to overcome the technical difficulties in studying the existence, stability, and bifurcation of the multiple endemic equilibria in high-dimensional phase space. We show that the model can also undergo transcritical bifurcations, saddle-node bifurcations, a pitchfork bifurcation, and Hopf bifurcations. The bifurcation diagrams and epidemiological interpretations are given. We conclude that when the islet reaches a critical size, the transmission cycle of the schistosomiasis japonica between wild rats Rattus norvegicus and snails Oncomelania hupensis could be established, which serves as a possible source of schistosomiasis transmission along the Yangtze River. © 2014 Society for Mathematical Biology. Source


Li Y.,Sun Yat Sen University | Xu H.,Sun Yat Sen University | Chen J.,Sun Yat Sen University | Gan W.,Sun Yat Sen University | And 3 more authors.
Parasitology Research | Year: 2012

Antigen 5 (Ag5) has been identified as a dominant component of cyst fluid of Echinococcus granulosus and is considered as a member of serine proteases family, which in other helminth, plays an important role in the egg hatch and larva invasion. However, whether Ag5 is expressed and secreted in all life stages is unknown. In this study, according to the sequence in GenBank, we cloned and sequenced the open reading frame (ORF) of Ag5 gene from the protoscolices of E. granulosus isolated from the sheep in Qinhai Province of China, and found several substitutions and a base insert and deletion in a short region near the stop code, leading to a frameshift mutation which is conserved with the homologue of other cestode. The ORF is 1,455 bp in length, encoding 484 amino acids with a secretory signal peptide. Bioinformatics analysis predicted several phosphorylation and myristoylation sites and a N-glycosylation site and a species-specific linear B epitope in the protein. The ORF was cloned into the plasmid pET28a(+) vector and expressed in Escherichia coli. The recombinant protein was purified by affinity chromatography. Anti-rEgAg5 antiserum was prepared in rats and used to analyze the localization of Ag5 in protoscolex and adult worm by immunofluorescence technique. Results demonstrated that the Ag5 is strongly expressed in the tegument of protoscolex and the embryonic membrane of egg and surface of oncosphere; meanwhile, it is also weakly expressed in tegument of the adult. This study showed that Ag5 is expressed in all stages of life cycle, secreted from the surface of the worm and may be anchored in membrane by its myristoylation sites; these characteristics make it a candidate antigen for diagnosis and vaccine for both intermediate and definitive hosts. © Springer-Verlag 2011. Source


Yang B.,Fudan University | Chen Y.,National Institute of Parasitic Disease | Wu L.,Fudan University | Xu L.,National Institute of Parasitic Disease | And 2 more authors.
American Journal of Tropical Medicine and Hygiene | Year: 2012

The seroprevalence of Entamoeba histolytica infection in the residents of seven provinces in China was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, C-Igl, of the parasites. A total of 1,312 serum samples were investigated. The positivity rates for these two antigens were 11.05% and 6.25%, respectively. There was no significant difference in the seropositivity to E. histolytica between men and women. We used a logistic regression model and maximal-likelihood methods to estimate the prevalence of E. histolytica infection from sequential serologic data. Seropositivity in Sichuan, Guizhou, and Sinkiang Provinces was higher than that in Beijing, Shanghai, and Qinghai Provinces. The present study provides an overview of seropositivity to E. histolytica infection in seven provinces in China and use the logistic regression model estimation method to achieve a more accurate measure of amebiasis prevalence. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene. Source


Chen Y.D.,National Institute of Parasitic Disease | Chen Y.D.,Collaborative Center for Malaria | Zhou C.H.,National Institute of Parasitic Disease | Zhou C.H.,Collaborative Center for Malaria | And 2 more authors.
Biomedical and Environmental Sciences | Year: 2012

Objective: To examine Clonorchis sinensis infection in China and evaluate the effectiveness of efforts to prevent and control it, two nationwide surveys were undertaken in 31 provinces, autonomous regions, and municipalities (PAMs) during 1988-92 (the 1990 survey) and during 2001-04 (the 2003 survey). Methods: During the period 2001-04, two sampling methods were applied. The first method repeated the stratified cluster random sampling used in the 1990 survey; the second method applied two-characteristic stratified cluster random sampling in 27 PAMs-the 2003 endemic area (EA) survey. The Kato-Katz thick smear method was used for the nationwide survey. Results: The infection rates of Clonorchis sinensis in the 1990 and 2003 surveys were 0.311 and 0.579, respectively. The infection rate was 2.40 in the 2003 EA survey, and it was estimated that 12.49 million people in China were infected with Clonorchis sinensis. Conclusion: The 2003 survey showed that the standardized infection rate of Clonorchis sinensis increased by 74.85 compared with the 1990 survey. The infection rate in males was higher than in females; the infection rate among people eating raw fish or eating out frequently was higher than among those who did not. © 2012 The Editorial Board of Biomedical and Environmental Sciences. Source

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