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El-Akhal F.,Health Diagnostic Laboratory | El-Akhal F.,University Sidi Mohammed Ben Abdellah | Guemmouh R.,University Sidi Mohammed Ben Abdellah | Ez Zoubi Y.,National Institute of Medicinal and Aromatic Plants | And 2 more authors.
Journal of Parasitology Research | Year: 2015

Background. Outbreaks of the West Nile virus infection were reported in Morocco in 1996, 2003, and 2010. Culex pipiens was strongly suspected as the vector responsible for transmission. In the North center of Morocco, this species has developed resistance to synthetic insecticides. There is an urgent need to find alternatives to the insecticides as natural biocides. Objective. In this work, the insecticidal activity of the extract of the local plant Nerium oleander, which has never been tested before in the North center of Morocco, was studied on larval stages 3 and 4 of Culex pipiens. Methods. Biological tests were realized according to a methodology inspired from standard World Health Organization protocol. The mortality values were determined after 24 h of exposure and LC50 and LC90 values were calculated. Results. The extract had toxic effects on the larvae of culicid mosquitoes. The ethanolic extract of Nerium oleander applied against the larvae of Culex pipiens has given the lethal concentrations LC50 and LC90 in the order of 57.57 mg/mL and 166.35 mg/mL, respectively. Conclusion. This investigation indicates that N. oleander could serve as a potential larvicidal, effective natural biocide against mosquito larvae, particularly Culex pipiens. © 2015 Fouad El-Akhal et al.

Chaouki W.,National Institute for Oncology | Leger D.Y.,Laboratory of Biochemistry | Eljastimi J.,Laboratory of Biochemistry | Beneytout J.-L.,Laboratory of Biochemistry | Hmamouchi M.,National Institute of Medicinal and Aromatic Plants
Pharmaceutical Biology | Year: 2010

Aristolochia baetica L. (Aristolochiaceae) and Origanum compactum Benth. (Lamiaceae) are native plants of Morocco used in traditional medicine. In order to systematically evaluate their potential activity on human breast cancer, four different polarity extracts from each plant were assessed in vitro for their antiproliferative effect on MCF-7 cells. As a result, several extracts of those plants showed potent cell proliferation inhibition on MCF-7 cells. Chloroform extract of A. baetica (IC50: 216.06±15 μg/mL) and ethyl acetate of O. compactum (IC50: 279.51±16 μg/mL) were the most active. Thin layer chromatography examination of the bioactive extracts of A. baetica and O. compactum showed the presence of aristolochic acid and betulinic acid, respectively. These results call for further studies of these extracts. © 2010 Informa UK Ltd.

El Hamsas El Youbi A.,National Institute of Medicinal and Aromatic Plants
American Journal of Therapeutics | Year: 2016

This study is designed to evaluate the analgesic and anti-inflammatory activities of aqueous extract (AE) of Cistus ladanifer L. leaves in experimental animal models. The central analgesic activity of C. ladanifer AE is studied using hot plate method in rats, and the acute anti-Inflammatory activity of C. ladanifer is investigated by rats paw edema induced by subplantar injection of 0.5% carrageenan into the right hind paw. Rats are pretreated with AE of C. ladanifer at different doses (150, 175, and 200 mg/kg, i.p.). The tramadol and indomethacin are used as reference drugs for analgesic and anti-inflammatory studies, respectively. Our results show that the AE of C. ladanifer exhibited anti-inflammatory and analgesic effects dose dependent. In anti-inflammatory activity, the AE of C. ladanifer at all doses reduced significantly the edema paw inflammation after carrageenan injection. Furthermore at 200 mg/kg, the effect of AE is highly important than that of other doses. In addition, the same AE demonstrates significant analgesic effect in thermal-induced pain model. So, this activity is proved by significant reduction of pain score after administration of AE at all doses. The nociception protection effects in this case are, respectively, 70.3%, 74.55%, and 93.33% after administration of AE of C. ladanifer at doses 150, 175, and 200 mg/kg b.w. The results of our findings suggest that AE of C. ladanifer has potential analgesic and anti-inflammatory activities with evidence of possible involvement of peripheral and central effects in its actions. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.

Jennan S.,University Sidi Mohammed Ben Abdellah | Jennan S.,National Institute of Medicinal and Aromatic Plants | Farah A.,National Institute of Medicinal and Aromatic Plants | Mahjoubi F.,University Sidi Mohammed Ben Abdellah
Journal of Materials and Environmental Science | Year: 2015

The present study reports on the extraction of phenolic compounds from aerial parts of Thymus hyemalis of Morocco. Box-Behnken Design (BBD), a widely used form of response surface methodology (RSM), was used to investigate the effect of process variables on the ultrasound-assisted extraction (UAE). Three independent variables including ethanol concentration (%), extraction time (min) and solvent-to-material ratio (mL/g) were studied. The results showed that the optimal UAE condition was obtained with an ethanol concentration of 72%, an extraction time of 37 min and a solvent-to-material ratio of 19 mL/g for total phenols, and an ethanol concentration of 70 %, an extraction time of 38 min and solvent-to-material ratio of 20 mL/g for the yield of extraction. The experimental values under optimal conditions were in good consistent with the predicted values.

Chahmi N.,University Sidi Mohammed Ben Abdellah | Chahmi N.,National Institute of Medicinal and Aromatic Plants | Anissi J.,University Sidi Mohammed Ben Abdellah | Anissi J.,Al Akhawayn University | And 5 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2015

Objective: To investigate antioxidant activity, total phenolic and flavonoid content of ethanol (E) and ethyl acetate (A) extracts of Inula viscosa aerial parts selected from three regions of Morocco (Imouzzer, Sefrou and Taounate). Methods: Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl, total antioxidant capacity and iron-reducing capacity. Total phenolic content was measured by Folin- Ciocalteu reagent. Results: All the extracts showed significant antioxidant activities and contained important levels of phenols. The ethanol extract (0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay, which was probably due to its high content of polyphenols (274.39±6.94) mg gallic acid equivalent/g dry extract. Total flavonoid content was found equal for all extracts. Conclusions: Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants. The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health. ©2015 by the Asian Pacific Journal of Tropical Biomedicine.

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