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Julio C.,National Institute Of Health Dr Ricardo Jorge Ip | Furtado C.,National Institute Of Health Dr Ricardo Jorge Ip | Rocha R.,National Institute Of Health Dr Ricardo Jorge Ip | Escobar C.,Hospital Prof Doutor Fernando Fonseca | And 2 more authors.
Parasitology | Year: 2015

Dientamoeba fragilis is an inhabitant of human gastrointestinal tract with a worldwide distribution. The first description considered this protozoan a rare and harmless commensal, since then it has struggled to gain recognition as a pathogen. Commercial multiplex real-time PCR was used to detect D. fragilis in fecal samples from hospitalized children (â1/218 years) with acute gastrointestinal disease, admitted to two hospitals of Lisbon area, with different demographic characteristics. A total of 176 children were studied, 103 (58.5%) male, 144 (81.8%) children between 0 and 5 years and 32 (18.2%) above 6 years old. The overall protozoa frequency considering the four tested microorganisms were 8.5% (15/176), and the most frequently found protozoan was D. fragilis, 6.3% (11/176). Dientamoeba fragilis frequency was higher among older children (21.9%), than younger children (2.8%), and greater in boys (6.8%) than in girls (5.5%). All positive children presented with diarrhoea associated with vomiting, fever and abdominal pain. Infection was associated with the age of children (P < 0.001), school attendance (P = 0.002) and consumption of certain foods (P = 0.014), e.g. cakes with crème and ham. The frequency of diantamoebiasis found in a cohort of hospitalized Portuguese children, with acute gastrointestinal disease, could be considered a very high value when compared with the protozoan frequency normally associated with this pathology. © 2015 Cambridge University Press.


Wijnhoven T.M.A.,World Health Organization | Van Raaij J.M.A.,National Institute for Public Health and the Environment | Van Raaij J.M.A.,Wageningen University | Spinelli A.,National Institute of Health | And 11 more authors.
Pediatric Obesity | Year: 2013

Background: Nutritional surveillance in school-age children, using measured weight and height, is not common in the European Region of the World Health Organization (WHO). The WHO Regional Office for Europe has therefore initiated the WHO European Childhood Obesity Surveillance Initiative. Objective: To present the anthropometric results of data collected in 2007/2008 and to investigate whether there exist differences across countries and between the sexes. © 2012 International Association for the Study of Obesity.

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