National Institute of Health Doctor Ricardo Jorge

Lisbon, Portugal

National Institute of Health Doctor Ricardo Jorge

Lisbon, Portugal
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Nascimento A.C.,National Institute of Health Doctor Ricardo Jorge | Mota C.,National Institute of Health Doctor Ricardo Jorge | Coelho I.,National Institute of Health Doctor Ricardo Jorge | Gueifao S.,National Institute of Health Doctor Ricardo Jorge | And 3 more authors.
Food Chemistry | Year: 2014

Quinoa, amaranth and purple corn are Andean cereals largely consumed in North of Argentina. Nutrient analysis with the purpose of inclusion in the Argentinean FCDB and e-search EuroFIR has become urgent matter. In this work proximate and mineral profile of Andean cereals cultivated in the North of Argentina were determined and compared with rice. Proximate analysis showed that Andean cereals have similar profile but significantly higher (p < 0.05) than rice. Andean cereals are rich sources of iron, copper, manganese and zinc and better than rice. Phosphorus and magnesium quinoa content could contribute up to 55% of consumers DRI. Andean cereals and rice are poor sources of potassium. To guarantee the interchange of data among users and producers of FCDB component values were obtained in compliance with EuroFIR guidelines for compilation process. Present work provides necessary information to FCDB users who wish to have access to food reference analytical parameters. © 2013 Elsevier Ltd. All rights reserved.


Mota C.,National Institute of Health Doctor Ricardo Jorge | Santos M.,National Institute of Health Doctor Ricardo Jorge | Matos A.S.,New University of Lisbon | Torres D.,University of Porto | Castanheira I.,National Institute of Health Doctor Ricardo Jorge
Food Chemistry | Year: 2016

Quinoa (Chenopodium quinoa), amaranth (Amaranthus caudatus) and buckwheat (Fagopyrum esculentum) represent the main protein source in several diets, although these pseudocereals are not currently present in the FCDB nutrient profile information. The aim of this work is to characterise the AA profile of these pseudocereals and compare them with rice. Total protein content revealed to vary from 16.3 g/100 g (quinoa Salta) to 13.1 g/100 g (buckwheat) and lower values were found in rice samples (6.7 g/100 g). For pseudocereals the most abundant essential AA was leucine. Quinoa-Salta evidences the highest leucine content (1013 mg/100 g) and the minor methionine content (199 mg/100 g). Buckwheat was the cereal with the highest phenylalanine content (862 mg/100 g). Rice (Oryza sativa) presents the lowest content for all AA. Results showed pseudocereals as the best source of AA. EuroFIR guidelines where strictly followed and proved to be a crucial tool to guarantee data interchangeability and comparability. © 2014 Elsevier Ltd. All rights reserved.


PubMed | University of Porto, National Institute of Health Doctor Ricardo Jorge and New University of Lisbon
Type: | Journal: Food chemistry | Year: 2015

Quinoa (Chenopodium quinoa), amaranth (Amaranthus caudatus) and buckwheat (Fagopyrum esculentum) represent the main protein source in several diets, although these pseudocereals are not currently present in the FCDB nutrient profile information. The aim of this work is to characterise the AA profile of these pseudocereals and compare them with rice. Total protein content revealed to vary from 16.3g/100g (quinoa Salta) to 13.1g/100g (buckwheat) and lower values were found in rice samples (6.7g/100g). For pseudocereals the most abundant essential AA was leucine. Quinoa-Salta evidences the highest leucine content (1013mg/100g) and the minor methionine content (199mg/100g). Buckwheat was the cereal with the highest phenylalanine content (862mg/100g). Rice (Oryza sativa) presents the lowest content for all AA. Results showed pseudocereals as the best source of AA. EuroFIR guidelines where strictly followed and proved to be a crucial tool to guarantee data interchangeability and comparability.


Pinheiro A.,University of Lisbon | Silva M.J.,University of Lisbon | Pavlu-Pereira H.,University of Lisbon | Florindo C.,University of Lisbon | And 7 more authors.
Gene | Year: 2016

Human pyruvate dehydrogenase complex (PDC) catalyzes a key step in the generation of cellular energy and is composed by three catalytic elements (E1, E2, E3), one structural subunit (E3-binding protein), and specific regulatory elements, phosphatases and kinases (PDKs, PDPs). The E1α subunit exists as two isoforms encoded by different genes: . PDHA1 located on Xp22.1 and expressed in somatic tissues, and the intronless . PDHA2 located on chromosome 4 and only detected in human spermatocytes and spermatids.We report on a young adult female patient who has PDC deficiency associated with a compound heterozygosity in . PDHX encoding the E3-binding protein. Additionally, in the patient and in all members of her immediate family, a full-length testis-specific . PDHA2 mRNA and a 5'UTR-truncated . PDHA1 mRNA were detected in circulating lymphocytes and cultured fibroblasts, being both mRNAs translated into full-length PDHA2 and PDHA1 proteins, resulting in the co-existence of both PDHA isoforms in somatic cells. Moreover, we observed that DNA hypomethylation of a CpG island in the coding region of . PDHA2 gene is associated with the somatic activation of this gene transcription in these individuals.This study represents the first natural model of the de-repression of the testis-specific . PDHA2 gene in human somatic cells, and raises some questions related to the somatic activation of this gene as a potential therapeutic approach for most forms of PDC deficiency. © 2016 Elsevier B.V.


Quinoa, amaranth and purple corn are Andean cereals largely consumed in North of Argentina. Nutrient analysis with the purpose of inclusion in the Argentinean FCDB and e-search EuroFIR has become urgent matter. In this work proximate and mineral profile of Andean cereals cultivated in the North of Argentina were determined and compared with rice. Proximate analysis showed that Andean cereals have similar profile but significantly higher (p<0.05) than rice. Andean cereals are rich sources of iron, copper, manganese and zinc and better than rice. Phosphorus and magnesium quinoa content could contribute up to 55% of consumers DRI. Andean cereals and rice are poor sources of potassium. To guarantee the interchange of data among users and producers of FCDB component values were obtained in compliance with EuroFIR guidelines for compilation process. Present work provides necessary information to FCDB users who wish to have access to food reference analytical parameters.

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