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Hassan M.U.,Pmas Arid Agriculture University | Akram Z.,Pmas Arid Agriculture University | Ajmal S.,Pmas Arid Agriculture University | Mukhtar T.,Pmas Arid Agriculture University | And 3 more authors.
Journal of Animal and Plant Sciences | Year: 2013

Peanut (Arachis hypogaea L.) belongs to family leguminosae and is one of the world's largest oilseed crops. Efficient tissue culture system is a pre-requisite for improvement of the crop through genetic engineering. Longitudinally halved cotyledons with removed plumule and radical were employed as explants to regenerate shoots in a previously determined best hormone combination. Shoots were inoculated singly or in the form of bunch on two different media in petri plates as well in jars. Shoot bunches in petri plates showed exceptionally high rooting and net survival efficiency on both media compared to all other combinations. Mechanical pressure exerted by low space in petri plate is the most probable reason for development of strong and extensive root system. Source


Nazir F.,National Institute of Genomics and Advanced Biotechnology | Mahmood-ul-Hassan,Pmas Arid Agriculture University | Akram Z.,Pmas Arid Agriculture University | Javed M.M.,Pmas Arid Agriculture University | And 3 more authors.
African Journal of Biotechnology | Year: 2011

Peanut (Arachis hypogaea L.) belongs to the family leguminosae and is one of the world's largest oilseed crops. This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI-2000, BARD-479 and BARD-92. Longitudinally, halved cotyledons with removed embryos were employed as explants. Among various tested combinations of BAP and NAA, the best combination was 4 and 0.1 mg/L respectively. BARI-2000 proved to be the best responsive variety for in vitro regeneration in terms of number of shoots/explant (133.3%) and number of rooted plants/explant (124.99%). The optimized protocol, which is the first one in the country, would be used to incorporate important traits in future breeding programmes of peanut in Pakistan. © 2011 Academic Journals. Source


Kamal T.,National Institute of Genomics and Advanced Biotechnology | Muneer A.,National Institute of Genomics and Advanced Biotechnology | Ali M.,National Institute of Genomics and Advanced Biotechnology | Fatima Z.,National Institute of Genomics and Advanced Biotechnology | Ullah A.,National Institute of Genomics and Advanced Biotechnology
Proceedings of 2015 12th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2015 | Year: 2015

Hydropericardium syndrome (HPS) is a contagious disease of young chicken caused by a DNA virus belonging to family Adenoviridae. It is an economically important poultry disease worldwide. In this study, 3 cell lines i.e., Vero, BHK-21 and QT-35, were compared in order to determine their susceptibility to Adenovirus. In the first phase of the study, primary chicken cultures i.e., fibroblast, liver and kidneys,were used for growth of the virus. Adenovirus showed cytopathic effects on liver primary culture only. It was confirmed by agar gel precipitation test (AGPT) against known positive HPS serum. Other cell lines didn't show any cytopathic effects (CPE) even after ten passages of virus on primary liver culture. In the second phase of study, the adapted virus was used to infect mono layer of Vero, BHK-21 and QT-35 cell lines. For adaptation of virus on cell lines, virus was repeatedly passaged five times on each cell lineseparately. Only QT-35 showed CPEs indicating a successful adaptation of HPS virus for growth on QT-35. Afterwards, viral load was determined by Tissue Culture Infective Dose TCID50. © 2015 IEEE. Source

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