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Gijang gun, South Korea

Getachew P.,Pukyong National University | Getachew P.,Addis Ababa Institute of Technology | Nam B.-H.,National Institute of Fisheries Science | Young Cho J.,Soonchunhyang University | Hong Y.-K.,Pukyong National University
Botanica Marina | Year: 2016

The stoloniferous hydrozoan Obelia geniculata frequently colonizes late-harvested blades of the aquacultured Saccharina japonica. To understand the corresponding changes at protein level, we compared the proteomic profiles in hydrozoan-colonized and healthy tissues. Our results showed that 107 and 75 protein spots were detected in colonized and healthy tissues, respectively. Among them, 7 and 16 identified proteins were significantly up- and down-regulated, respectively. Up-regulated proteins of cell-division cycle 46/minichromosome maintenance protein 5 and glutamyl-tRNA reductase were found mostly in hydrozoan-colonized tissues but were rare in healthy tissues. Microcompartments protein, carboxysome shell peptide, biotin synthetase, serine/arginine-rich splicing factor and a two-component response regulator were up-regulated in hydrozoan-colonized tissues. However, downregulated proteins of phosphoglycerate kinase, expansin 6, translation initiation factor 3, calcium/calmodulin-dependent protein kinase II inhibitor 2 and 50S ribosomal protein L1P were found mostly in healthy tissues but rare in hydrozoan-colonized tissues. Transmembrane protein, protoporphyrinogen oxidase, dual oxidase 2, PIH1 domain-containing protein 2, GTPase-activating protein alpha, threonyl-tRNA synthetase, flavanone 3-hydroxylase, uncoupling protein 3, bromoperoxidase 7, peptide release factor 1, and interaptin were down-regulated in hydrozoan-colonized tissues. Most of the up- and down-regulated proteins are known to be related to stress control, signal transduction and photosynthesis. © 2016 by De Gruyter 2016. Source

Thulasitha W.S.,Jeju National University | Umasuthan N.,Jeju National University | Whang I.,Jeju National University | Nam B.-H.,National Institute of Fisheries Science | Lee J.,Jeju National University
Fish and Shellfish Immunology | Year: 2016

In this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream Oplegnathus fasciatus (OfGal-1). Galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. They have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. OfGal-1 cDNA comprised of 993 bp with an open reading frame of 408 bp that encodes 135 amino acids. A single carbohydrate recognition domain was present in the OfGal-1 amino acid sequence. The sequence comparison by multiple and pairwise alignments and the phylogenetic tree emphasized the strong evolutionary conservation of Gal-1. The typical β-sandwich structure was identified from the predicted tertiary structure. The constitutive expression of mRNA transcripts was detected in a wide range of tissues examined, with the highest expression in the heart. Immune challenges with live bacteria (Edwardsiella tarda and Streptococcus iniae), rock bream irido virus, and mitogens (lipopolysaccharide and poly I:C) modulated the expression of OfGal-1 mRNAs in the gills, head kidney, and liver. The recombinant OfGal-1 (rOfGal-1) strongly agglutinatinated the human erythrocytes, and this hemagglutination was inhibited by lactose and d-galactose. A wide range of bacteria (S. iniae, S. parauberis, Escherichia coli, Edwardsiella tarda, Vibrio anguillarum, Vibrio harveyi, and Vibrio tapetis) and a ciliate (Miamiensis avidus) were also effectively recognized by rOfGal-1. Significant antiviral activity against rock bream irido virus was also demonstrated by rOfGal-1. Collectively, results from the present study indicate that OfGal-1 can recognize a wide range of microbes and is a vital pattern recognition receptor in the innate immune system of rock bream. © 2016 Elsevier Ltd. Source

Jeong J.-H.,Weather Radar Center | Lee D.-I.,Pukyong National University | Wang C.-C.,National Taiwan Normal University | Han I.-S.,National Institute of Fisheries Science
Natural Hazards and Earth System Sciences | Year: 2016

An extreme-rainfall-producing mesoscale convective system (MCS) associated with the Changma front in southeastern South Korea was investigated using observational data. This event recorded historic rainfall and led to devastating flash floods and landslides in the Busan metropolitan area on 7 July 2009. The aim of the present study is to analyse the influences for the synoptic and mesoscale environment, and the reasons that the quasi-stationary MCS causes extreme rainfall. Synoptic and mesoscale analyses indicate that the MCS and heavy rainfall occurred in association with a stationary front which resembled a warm front in structure. A strong southwesterly low-level jet (LLJ) transported warm and humid air and supplied the moisture toward the front, and the air rose upwards above the frontal surface. As the moist air was conditionally unstable, repeated upstream initiation of deep convection by back-building occurred at the coastline, while old cells moved downstream parallel to the convective line with training effect. Because the motion of convective cells nearly opposed the backward propagation, the system as a whole moved slowly. The back-building behaviour was linked to the convectively generated cold pool and its outflow boundary, which played a role in the propagation and maintenance of the rainfall system. As a result, the quasi-stationary MCS caused a prolonged duration of heavy rainfall, leading to extreme rainfall over the Busan metropolitan area. © 2016 Author(s). Source

Kim K.I.,East Sea Fisheries Research Institute | Kwon W.J.,Pukyong National University | Kim Y.C.,Pukyong National University | Kim M.-S.,National Institute of Fisheries Science | And 2 more authors.
Aquaculture | Year: 2016

Surveillance of aquatic animal viruses in hosts and seawater is very important to control and prevent the spread of aquatic animal diseases. And also the investigations for shellfish as play role in vector or carrier species, or a candidate host are required. Primer sets for multiplex nested polymerase chain reaction were developed to simultaneously detect aquatic animal viruses (megalocytivirus and white spot syndrome virus as DNA viruses, and viral hemorrhagic septicemia virus, viral nervous necrosis virus [VNNV], and marine birnavirus as RNA viruses) from seawater and shellfish. The multiplex nested PCR amplified each virus specifically with a detection limit of about 10 copies of viral particles and facilitated detection of several aquatic animal viruses from seawater and shellfish. The detection rates of viruses in shellfish samples were not significantly different between areas, except for VNNV which showed much lower positive results in eastern sea area. But, several viruses co-existed in shellfish regardless of the sampling site and time. These results revealed that shellfish might accumulate several viruses over the long-term and could play a role as a bio-indicator or reservoir of viruses derived from aquatic animals. Statement of relevance: This article is related to field of aquatic animal disease. In this study, we developed multiplex nested PCR assay for detection of aquatic animal viruses from seawater and shellfish. And we found several viruses co-existed in shellfish for long-term, suggesting that shellfish may act as a bio-indicator or reservoir of viruses derived from aquatic animals. © 2015 Elsevier B.V. Source

Postprandial ammonia excretion and oxygen consumption in olive flounder Paralichthys olivaceus fed two different feed types, moist pellet (MP) and expanded pellet (EP) diets, to satiation were determined at 12°C, 15°C, 20°C, and 25°C for 48 h. The ammonia excretion and oxygen consumption rates increased with increasing water temperature. However, the postprandial times for the maximum rates of ammonia excretion and oxygen consumption were shortened from 12 h to 6 h after feeding with increasing water temperature. The ammonia excretion and oxygen consumption rates of fish fed EP were significantly higher (P < 0.05) than those fed MP at 12 h post-feeding both for 12°C and 15°C. The highest (P < 0.05) weight-specific ammonia excretion rates at 12°C were observed in fish fed EP and MP at 12.1 mg NH3-N kg-1h-1 and 8.7 mg NH3-N kg-1h-1, respectively, for 12 h and 9 h after feeding. The highest (P < 0.05) weight-specific oxygen consumption rates at 12°C were observed in fish fed EP and MP at 116.4 mg kg-1h-1 and 101.0 mg kg-1h-1, respectively, for 12 h after feeding. The highest ammonia excretion rates at 25°C in fish fed EP and MP increased to 16.9 mg NH3-N kg-1h-1 and 18.3 mg NH3-N kg-1h-1, respectively, for 6 h after feeding. The highest (P < 0.05) weight-specific oxygen consumption rates at 25°C were observed in fish fed EP and MP at 184.3 mg O2 kg-1h-1 and 197.3 mg O2 kg-1h-1, respectively. These data are extremely valuable for the design of biofilters and development of effluent treatment technologies for land-based flounder farms. © 2015 The Korean Society of Fisheries and Aquatic Science. Source

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