National Institute of Care and Research on Ageing

Ancona, Italy

National Institute of Care and Research on Ageing

Ancona, Italy

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Meme L.,Marche Polytechnic University | Santarelli A.,Marche Polytechnic University | Santarelli A.,National Institute of Care and Research on Ageing | Marzo G.,University of L'Aquila | And 8 more authors.
International Journal of Immunopathology and Pharmacology | Year: 2014

Since raloxifene, a drug used in osteoporosis therapy, inhibits osteoclast, but not osteoblast functions, it has been suggested to improve recovery during implant surgery. The present paper describes an effective method to link raloxifene, through a covalent bond, to a nano-Hydroxyapatite-based biomaterial by interfacing with (3-aminopropylKIYiethoxysilane as assessed by Infra Red-Fourier Transformed (IR-FT) spectroscopy and Scanning Electron Microscope (SEM). To evaluate the safety of this modified new material, the vitality of osteoblast-like celis cultured with the new biomaterial was then investigated. Raloxifene-conjugated HA- biomaterial has been shown to be a safe material easy to obtain which could be an interesting starting point for the use of a new functional biomaterial suitable in bone regeneration procedures.


Lo Muzio L.,University of Foggia | Santarelli A.,Marche Polytechnic University | Santarelli A.,National Institute of Care and Research on Ageing | Orsini G.,Marche Polytechnic University | And 12 more authors.
European Journal of Inflammation | Year: 2013

Bone resorption in edentulous regions often results in inadequate ridge for implant osseointegration. In order to overcome this problem, the use of osteoconductive biomaterials has been proposed as a carrier for different types of pharmacological molecules. Since raloxifene, a drug used in osteoporosis therapy, inhibits the osteoclast, but not osteoblast functions, it has been suggested to improve recovery during implant surgery. The present work evaluated in vitro the effect of raloxifene on two different cell populations: the human osteoblast-like cells (MG63) and osteoblasts derived from rat calvaria (MC3T3E1). The morpho-functional investigations carried out showed a different behavior of the two cell lines. Raloxifene showed a stimulatory effect towards MG63 cell proliferation with a significant increase in cell viability after 7 days of culture. On the contrary, MC3T3-E1 cells showed a significant reduction in cell viability, when compared with the same cells at 72 h, or with the control cell population. The predominantly proliferative effect of raloxifene on MG63 cells is partly confirmed by the reduction of alkaline phosphatase activity, an early marker of osteoblast differentiation. The different effect of raloxifene on osteoblastic population in relationship to the type and age of the cell is an issue that needs further investigation. Copyright © by BIOLIFE, s.a.s.


Santarelli A.,Marche Polytechnic University | Santarelli A.,National Institute of Care and Research on Ageing | Mascitti M.,Marche Polytechnic University | Orsini G.,Marche Polytechnic University | And 9 more authors.
Journal of Biological Regulators and Homeostatic Agents | Year: 2014

Calcium phosphate ceramics have been applied in bone replacement for several decades due to their excellent biocompatibility, bioactivity, osteoconductivity and mechanical strength. Several studies have demonstrated that porous hydroxyapatite (HA) is an excellent scaffold for osteogenic proliferation and differentiation of the osteoprogenitor cells. However, different methods of synthesis and production of HA ceramic-based materials may have considerable effect on the physical and biological properties. In the present work, two hydroxyapatite-based materials, a natural hydroxyapatite ceramic of bovine origin and a synthetic nano-cristalline hydroxyapatite were tested in vitro with MG63 cell line. The results displayed that both the materials demonstrated a good biocompatibility. The immunocytochemical stain revealed a different positivity of the osteogenic markers between the cultures with the biomaterials, and the control culture. Western blot data confirmed the immunocytochemical stain. Both the materials tested in the present study demonstrated a good biocompatibility with the osteoblastic cells allowing, at the same time, the osteogenic differentiation, and they may be useful in clinical use. Copyright © by BIOLIFE, s.a.s.


Gagliardi C.,National Institute of Care and Research on Ageing | Spazzafumo L.,National Institute of Care and Research on Ageing | Papa R.,National Institute of Care and Research on Ageing | Marcellini F.,National Institute of Care and Research on Ageing
International Journal of Aging and Human Development | Year: 2012

The present study examines the leisure style and leisure satisfaction of a sample of older people at baseline and after a period of 5 years. Three groups were identified by factorial and cluster analyses and labelled under the headings of: Organised Style, Surrounding Style and Indoor Style. Each group represented a different typology of leisure, according to the demand for physical and organizational commitment. Results indicated that the Organized Style group was the most active and satisfied with their leisure time, and differed from the other two by being predominantly male, younger, better educated and in good health. Follow-up analyses revealed a decline in the size of both the Organised Style and Surrounding Style groups, and an increase in the size of the less active group, suggesting that advancing very old age with deteriorating health influenced older peoples' choices toward an increment of indoor activities. This pattern may be softened through the implementation of mechanisms of selection and optimisation. © 2012, Baywood Publishing Co., Inc.


PubMed | National Institute of Care and Research on Ageing
Type: Comparative Study | Journal: International journal of aging & human development | Year: 2013

The present study examines the leisure style and leisure satisfaction of a sample of older people at baseline and after a period of 5 years. Three groups were identified by factorial and cluster analyses and labelled under the headings of: organised style, surrounding style and indoor style. Each group represented a different typology of leisure, according to the demand for physical and organizational commitment. Results indicated that the Organized Style group was the most active and satisfied with their leisure time, and differed from the other two by being predominantly male, younger, better educated and in good health. Follow-up analyses revealed a decline in the size of both the organised style and surrounding style groups, and an increase in the size of the less active group, suggesting that advancing very old age with deteriorating health influenced older peoples choices toward an increment of indoor activities. This pattern may be softened through the implementation of mechanisms of selection and optimisation.

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