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Kumar H.,Central University of Bihar | Priya P.,Central University of Bihar | Singh N.,Central University of Bihar | Kumar M.,Central University of Bihar | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2016

Euryale ferox Salisbury is an important aquatic food plant cultivated largely in eastern India. E. ferox is a monotypic genus, and breeding programmes have mostly relied on the variability present in the primary gene pool. Knowledge of the genetic structure of the population is limited, and there are very few reports available on the genetic diversity of E. ferox. In this study, comprehensive research on the genetic diversity of 16 germplasms of E. ferox was carried out using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Out of 320 RAPD and 95 ISSR primers screened initially, 61 primers (40 RAPD and 21 ISSR) gave reproducible bands and were selected for further work. Amplification of the 40 RAPD primers gave 533 polymorphic bands with an average of 13.32 polymorphic bands per primer. The percentage of polymorphism ranged from 37.5 to 100, with an average of 88.3 %. The 21 ISSR primers produced 259 bands, of which 214 were polymorphic, with an average of 10.19 polymorphic bands per primer. The percentage of polymorphism using ISSR primers ranged from 50 to 100, with a mean of 82.6 %. Jaccard’s coefficient ranged from 0.45 to 0.69 (RAPD), 0.50 to 0.77 (ISSR) and 0.48 to 0.71 (RAPD and ISSR). Molecular characterization of different germplasms of E. ferox not only is essential for its conservation but also can be used in further breeding programmes. © 2016 Springer Science+Business Media New York Source


Choudhary V.K.,Indian Council of Agricultural Research | Choudhary V.K.,ICAR National Institute of Biotic Stress Management | Dixit A.,ICAR National Institute of Biotic Stress Management | Chauhan B.S.,University of Queensland
Crop and Pasture Science | Year: 2016

Intercropping provides opportunity to harness available resources. Thus, maize intercropped with soybean or peanut (groundnut) was tested with 1:1, 1:2, and 1:5 row proportions along with sole plantings of each crop to measure resource capture and resource-use efficiency. Results revealed that sole peanut had 60% higher maize-equivalent yield and 55% better production efficiency, followed by the 1:5 row proportion of maize-peanut, over sole maize. Intercropping increased land-use efficiency by 17-53% and land-equivalent coefficient by 0.21-0.56. The relative crowding coefficient was 39% higher with 1:2 maize-soybean, whereas the monetary advantage index was the highest (US$107) with the 1:5 maize-peanut. Nitrogen (N) and potassium (K) uptakes by maize were 42.9% and 38.2%, respectively, higher with 1:5 maize-soybean, whereas phosphorus (P) uptake was 64% higher with 1:5 maize-peanut. However, the least amounts of N, P and K were mined overall with 1:5 of maize-soybean. Soil moisture content was improved by 24% and solar radiation interception by 37.8% with soybean alone over maize alone, and these parameters increased with higher row proportions of soybean. The energy parameters also improved with the 1:5 row proportions of maize-soybean or peanut intercropping. This study provides a basis for efficient resource use by maize-soybean (or peanut) intercropping system. © 2016 CSIRO. Source


Sivalingam P.N.,ICAR Central Institute for Arid Horticulture | Sivalingam P.N.,ICAR National Institute of Biotic Stress Management | Samadia D.K.,ICAR Central Institute for Arid Horticulture | Singh D.,ICAR Central Institute for Arid Horticulture | Chauhan S.,ICAR Central Institute for Arid Horticulture
Journal of Horticultural Science and Biotechnology | Year: 2016

Prosopis cineraria (L.) Druce, commonly called khejri, is an important multipurpose, leguminous tree of the Thar Desert. ‘Thar Shoba’ is a variety of khejri without thorns which was developed as a vegetable. In order to develop markers for varietal identification, 80 RAPD primers were tested, of which nine were polymorphic between ‘Thar Shoba’ and trees in a natural population of khejri. Phenetic analysis revealed that ‘Thar Shoba’ formed a separate cluster from the natural population with similarity coefficients of 0.57-0.78. The 10-mer primers OPBE05, OPBA13, OPA12, and OPA14 were each found to differentiate ‘Thar Shoba’ from the natural population by one amplicon. These differential amplicons were sequenced and SCAR markers were developed based on the sequences that differentiated ‘Thar Shoba’ from the natural population. Natural populations of khejri from different regions of the Thar Desert, and clonally multiplied ‘Thar Shoba’ plants in the ICAR-CIAH bud-wood genebank, were used to validate these primers. These markers will be useful in further developing the bud-wood genebank, and for certification, identification, and protection of the khejri variety ‘Thar Shoba’. © 2016 The Journal of Horticultural Science & Biotechnology Trust. Source

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